Yang七七seo

Page1KELVINH.
LEE–GoreProfessorChemical&BiomolecularEngineeringDept.
AmmonPinizzottoBiopharm.
InnovationCenterTel:302-831-0344UniversityofDelaware590Avenue1743Fax:302-831-4841Email:KHL@UDEL.
EDUNewarkDE19713USAWeb:WWW.
LEELAB.
ORGEDUCATION:Ph.
D.
ChemicalEngineering(minorinBiology)CaliforniaInstituteofTechnologyM.
S.
ChemicalEngineering4/954/93CaliforniaInstituteofTechnologyB.
S.
E.
ChemicalEngineeringandCertificateinEngineeringBiology6/91PrincetonUniversityPROFESSIONALEXPERIENCE:NationalInstituteforInnovationinManufacturingBiopharmaceuticalsInstituteDirector1/17-presentUniversityofDelawareGoreProfessorofChemical&BiomolecularEngineering8/07-presentSeniorResearchScientist,ChristianaCareHealthSystem9/11-presentDirector,NSFIGERT:SystemsBiologyofCellsinEngineeredEnvironments7/12-7/19Director,DelawareBiotechnologyInstitute9/08-1/17CornellUniversityProfessorSchoolofChemical&BiomolecularEngineering4/07–8/07TheSamuelC.
andNancyM.
FlemingProfessorofMolecularandCellBiology2005-2007DirectoroftheInstituteforBiotechnologyandLifeScienceTechnologies2005-2007DirectoroftheNewYorkStateCenterforLifeScienceEnterprise2005-2007AssociateProfessor,SchoolofChemicalandBiomolecularEngineering4/03–4/07AssistantProfessor,SchoolofChemicalandBiomolecularEngineering11/97–4/03Education/TrainingPostdoctoralScholar,BiologyDivision,CaliforniaInstituteofTechnology9/95–10/97GraduateResearchAsst.
andResearchFellow,Inst.
Biotech.
4/93-9/95SwissFederalInstituteofTechnology(Zürich,Switzerland)GraduateResearchAsst.
,ChemicalEngineering/MolecularBiotech.
9/91-4/93CaliforniaInstituteofTechnologyHONORSNationalScienceFoundationGraduateFellowship(1991-1994)DuPontYoungProfessor(1999-2002)NationalScienceFoundationCAREERAward(1999-2003)J.
P.
andMaryBarger'50CollegeofEngineeringExcellenceinTeachingAward2000MITTechnologyReviewTop100InnovatorsintheWorldinBusiness&Technology2002JayBaileyYoungInvestigatorBestPaperAwardinMetabolicEngineering2002CamilleDreyfusTeacher-ScholarAward2003KelvinH.
LeePage2CornellProvost'sRonayandRichardMenschelAwardforDistinguishedScholarship2004.
DistinguishedProfessor,NYSOfficeofScience,TechnologyandAcademicResearch2004.
SamuelC.
andNancyM.
FlemingProfessorofMolecularandCellBiology2005.
GoreProfessorofChemicalEngineering2007.
InauguralWinneroftheBiochemicalEngineeringJournalYoungInvestigatorAward,2009.
ElectedFellowofAmericanInstituteofMedicalandBiologicalEngineers,2010.
ElectedFellowofAmericanAssociationfortheAdvancementofScience,2011.
InauguralWinneroftheAmericanElectrophoresisSocietyAward,2011.
BioprocessInternationalUpstreamCollaborationoftheDecadeFinalist,2012.
ProfessionalProgressAwardforOutstandingProgressinChemicalEngineering,AIChE,2013.
DelawareScientistoftheYear,DelawareBioscienceAssociation,2016.
BiotechnologyandBioengineeringElmerGadenAwardforOutstandingPublication,2017.
ACSBIOTMarvinJohnsonAwardinMicrobialandBiochemicalTechnology.
2018.
Peer-ReviewedJournalPublications.
1)MGHarrington,KHLee,TZewert,MYun,JEBailey,LEHood(1993).
Mechanicalprecisionintwo-dimensionalelectrophoresiscanimproveproteinspotpositionalreproducibility,AppliedandTheoreticalElectrophoresis3:347-353.
2)MGHarrington,KHLee,JEBailey,LEHood(1994).
Sponge-likeElectrophoresisMedia-MechanicallyStrongMaterialsCompatibleWithOrganic-Solvents,Polymer-Solutionsand2-DimensionalElectrophoresis,Electrophoresis15:187-194.
3)WARenner,KHLee,VHatzimanikatis,JEBailey,HEppenberger(1995).
RecombinantCyclinEExpressionActivatesProliferationandObviatesSurfaceAttachmentofChineseHamsterOvary(CHO)CellsinProtein-FreeMedium,BiotechnologyandBioengineering47:476-482.
4)VHatzimanikatis,KHLee,WARenner,JEBailey(1995).
AMathematicalModefortheG1/STransitionoftheMammalianCell,BiotechnologyLetters17:669-674.
5)KHLee,ASburlati,WRenner,JEBailey(1996).
DeregulatedExpressionofClonedTranscriptionFactorE2F-1inChineseHamsterOvaryCellsShiftsProteinPatternsandActivatesGrowthinProtein-FreeMedium,BiotechnologyandBioengineering50:273-279.
6)KHLee,MGHarrington,JEBailey(1996).
Two-DimensionalElectrophoresisofProteinsasaToolintheMetabolicEngineeringofCellCycleRegulation,BiotechnologyandBioengineering50:336-340.
7)GHsich,KKenney,CJGibbs,KHLee,MGHarrington(1996).
The14-3-3BrainProteininCerebrospinalFluidasaMarkerforTransmissibleSpongiformEncephalopathies,NewEnglandJournalofMedicine335:924-930.
PMID:87824998)KHLeeandMGHarrington(1996).
PremortemDiagnosisofCreutzfeldt-JakobDiseasebyCerebrospinalFluidAnalysis,TheLancet348:887.
PMID:88268199)RGWill,MZeidler,PBrown,MHarrington,KHLee,KKenney(1996).
CerebrospinalFluidTestforNewVariantCreutzfeldt-JakobDisease,TheLancet348:955.
10)JEBailey,ASburlati,VHatzimanikatis,KHLee,WRenner,PTsai(1996).
InverseMetabolicEngineering:AStrategyforDirectedGeneticEngineeringofUsefulPhenotypes,BiotechnologyandBioengineering52:109-121.
Reprintedinaspecialissueas79:568-579(2002).
11)PKachroo,KHLee,CSchwerdel,JEBailey,BChattoo(1997).
Analysisofhost-inducedresponseinthericeblastfungusMagnaporthegriseausingtwo-dimensionalpolyacrylamidegelelectrophoresis,1.
30.
20KelvinH.
LeePage3Electrophoresis18:163-169.
12)KHLeeandMGHarrington(1997).
TheAssayDevelopmentofaMolecularMarkerforTransmissibleSpongiformEncephalopathies,Electrophoresis18:502-506.
13)KHLeeandMGHarrington(1997).
14-3-3andBSE,TheVeterinaryRecord140:206-207.
14)LHChoe,WChen,KHLee(1999).
ProteomeAnalysisofFisOverexpressioninE.
coli,Electrophoresis20:798-805.
PMID:1034425015)VHatzimanikatis,LHChoe,KHLee(1999).
Proteomics:TheoreticalandExperimentalConsiderations,BiotechnologyProgress15:312-318.
PMID:1035624816)VHatzimanikatis,KHLee(1999).
DynamicalAnalysisofGeneNetworksRequiresBothmRNAandProteinExpressionInformation,MetabolicEngineering1:275-281(E1-E7).
17)VHatzimanikatis,KHLee,JEBailey(1999).
AMathematicalDescriptionofRegulationoftheG1-STransitionoftheMammalianCellCycle,BiotechnologyandBioengineering65:631-637.
18)LHChoe,KHLee(2000).
AComparisonofThreeCommerciallyAvailableIsoelectricFocusingUnitsforProteomeAnalysis:theMultiphor,theIPGphorandtheProteanIEFCell,Electrophoresis21:9931000.
PMID:1076878619)PSLee,KHLee(2000).
GenomicAnalysis,CurrentOpinioninBiotechnology11:171-175.
20)MJDutt,KHLee(2000).
ProteomicAnalysis,CurrentOpinioninBiotechnology11:176-179.
PMID:1075375921)VHatzimanikatis,DCollins,SLawrence,SBrowning,KHLee(2000).
Bioinformatics,GenomicsandtheChemicalEngineer–aPerspective,ChemicalEngineeringEducation34:346-349.
22)MJDuttandKHLee(2001).
TheScaledVolumeasanImageAnalysisVariableforDetectingChangesinProteinExpressionLevelsbySilverStain,Electrophoresis22:1627-1632.
PMID:1142521823)KHLee(2001).
Proteomics:ATechnology-DrivenandaTechnology-LimitedDiscoveryScience,TrendsinBiotechnology19:217-222.
PMID:1135628324)LHChoe,AGreen,RKnight,EThompsonandKHLee(2002).
ApolipoproteinEandOtherCerebrospinalFluidProteinsDifferentiateAntemortemVariantCreutzfeldt-JakobFromAntemortemSporadicCreutzfeldt-JakobDisease,Electrophoresis23:2242-2246.
PMID:1221022825)LHChoe,MJDutt,NRelkinandKHLee(2002).
StudiesofPotentialCerebrospinalFluidMolecularMarkersforAlzheimer'sDisease,Electrophoresis23:2247-2251.
PMID:1221022926)EHayduk,LHChoeandKHLee(2002).
Proteomictoolsindiscovery-drivenscience,CurrentScience83:840-844.
27)CLi,WCLee,andKHLee(2003).
AffinitySeparationsUsingMicrofabricatedMicrofluidicDevices:InsituPhotopolymerizationandUseinProteinSeparations,BiotechnologyandBioprocessEngineering8:240-245.
28)LBShaw,RZiaandKHLee(2003).
Totallyasymmetricexclusionprocesswithextendedobjects:Amodelforproteinsynthesis,PhysicalReviewEStatisticalNonliearSoftMatterPhysics68:021910.
29)KAggarwalandKHLee(2003).
FunctionalGenomicsandProteomicsasaFoundationforSystems1.
30.
20KelvinH.
LeePage4Biology,BriefingsinFunctionalGenomicsandProteomics2:175-184.
30)LHChoeandKHLee(2003).
AQuantitativeandQualitativeMeasureofIntralaboratoryTwo-dimensionalGelReproducibilityandtheEffectsofSamplePreparation,SampleLoad,andImageAnalysis,Electrophoresis24:3500-3507.
PMID:1459569731)EJFinehoutandKHLee(2003).
AComparisonofAutomatedIn-GelDigestMethodsforFemtomoleLevelSamples,Electrophoresis24:3508-3516.
32)PSLeeandKHLee(2003).
Escherichiacoli—aModelSystemThatBenefitsFromandContributestotheEvolutionofProteomics,BiotechnologyandBioengineering84:801-814.
PMID:1470812133)AMehra,KHLeeandVHatzimanikatis(2003).
InsightsintotheRelationBetweenmRNAandProteinExpressionPatterns.
I.
TheoreticalConsiderations,BiotechnologyandBioengineering84:822-833.
34)PSLee*,LBShaw*,LHChoe*,AMehra,VHatzimanikatisandKHLee(2003).
InsightsintotheRelationBetweenmRNAandProteinExpressionPatterns.
II.
ExperimentalObservationsinEscherichiacoli,BiotechnologyandBioengineering84:834-841.
PMID:1470812435)WCLeeandKHLee(2004).
ApplicationsofAffinityChromatographyinProteomics,AnalyticalBiochemistry324:1-10.
36)LBShaw,AKolomeiskyandKHLee(2004).
LocalInhomogeneityinAsymmetricSimpleExclusionProcesseswithExtendedObjects,JournalofPhysicsA:MathematicalandGeneral37:2105-2113.
37)EJFinehoutandKHLee(2004).
AnIntroductiontoMassSpectrometryApplicationsinBiologicalResearch,BiochemistryandMolecularBiologyEducation32:93-100.
38)EHayduk,LHChoeandKHLee(2004).
ATwo-dimensionalElectrophoresisMapofChineseHamsterOvaryCellProteinsBasedonFluorescenceStaining,Electrophoresis25:2545-2556.
PMID:1530077539)EJFinehout,ZFranck,andKHLee(2004).
Towardstwo-dimensionalelectrophoresismappingofthecerebrospinalfluidproteomefromasingleindividual,Electrophoresis25:2564-2575.
40)LShaw,JSethna,andKHLee(2004).
Meanfieldapproachestothetotallyasymmetricexclusionprocesswithquencheddisorderandlargeparticles,PhysicalReviewEStatisticalNonlinearSoftMatterPhysics70:021901.
41)CLiandKHLee(2004).
AffinitydepletionofalbuminfromhumancerebrospinalfluidusingCibacronblue-3G-A-derivatizedphotopatternedcopolymerinamicrofluidicdevice,AnalyticalBiochemistry333:381-388.
42)YCPark,SGKim,KHLee,KPark,andJHSeo(2004).
Fed-batchproductionofD-ribosefromsugarmixturesbytransketolasedeficientBacillussubtilisSPK1,AppliedMicrobiologyandBiotechnology66:297-302.
43)KSBrown,CCHill,GACalero,CMyers,KHLee,JPSethnaandRACerione(2004).
Thestatisticalmechanicsofcomplexsignalingnetworks:nervegrowthfactorsignaling,PhysicalBiology1:184-195.
44)PSLeeandKHLee(2005).
EngineeringHlyAHypersecretioninEscherichiacoliBasedonProteomicandMicroarrayAnalyses,BiotechnologyandBioengineering89:195-205.
45)EJHaydukandKHLee(2005).
CytochalasinDImprovesHeterologousProteinProductivityinAdherentChinesehamsterovarycells,BiotechnologyandBioengineering90:354-364.
1.
30.
20KelvinH.
LeePage546)CLi,YYang,HGCraigheadandKHLee(2005).
IsoelectricFocusinginCyclicOlefinCopolymerMicrofluidicChannelsCoatedbyPolyacrylamideUsingaUVPhotograftingMethod,Electrophoresis26:1800-1806.
47)KAggarwal,LHChoeandKHLee(2005).
QuantitativeAnalysisofProteinExpressionUsingAmine-SpecificIsobaricTagsinEscherichiacoliCellsExpressingrhsAElements,Proteomics5:2297-2308.
PMID:1588718448)EFinehout,JCantorandKHLee(2005).
KineticCharacterizationofSequencingGradeModifiedTrypsin,Proteomics5:2319-2321.
49)EJFinehout,ZFranckandKHLee(2005).
ComplementProteinIsoformsinCSFasPossibleBiomarkersforNeurodegenerativeDisease,DiseaseMarkers21:93-101.
50)LHChoe*,KAggarwal*,ZFranckandKHLee(2005).
AComparisonoftheConsistencyofProteomeQuantitationUsingTwo-DimensionalElectrophoresisandShotgunIsobaricTagginginEscherichiacoliCells,Electrophoresis26:2437-2449.
PMID:1592436251)JDiao,DRen,JREngstrom,andKHLee(2005).
ASurfaceModificationStrategyonSiliconNitrideforDevelopingBiosensors,AnalyticalBiochemistry343:322-328.
52)YYang,CLi,KHLee,andHGCraighead(2005).
CouplingOn-ChipSolidPhaseExtractiontoElectrosprayMassSpectrometryThroughanIntegratedElectrosprayTip,Electrophoresis26:36223630.
53)YYang,CLi,JMakeoka,KHLeeandHGCraighead(2005).
APolymericMicrochipwithIntegratedTipsandinsituPolymerizedMonolithforElectrosprayMassSpectrometry,LabonaChip5:869-876.
54)RKuczenski,KAggarwal,andKHLee(2005).
ImprovedUnderstandingofGeneExpressionRegulationUsingSystemsBiology,ExpertReviewofProteomics2:915-924.
55)MD'Ascenzo,NRRelkin,andKHLee(2005).
Alzheimer'sDiseaseCerebrospinalFluidBiomarkerDiscovery:AProteomicsApproach,CurrentOpinioninMolecularTherapeutics7:557-564.
PMID:1637240556)LHChoe,BWerner,andKHLee(2006).
Two-dimensionalProteinElectrophoresis:FromMolecularPathwayDiscoverytoBiomarkerDiscoveryinNeurologicalDisorders,NeuroRX3:327-335.
PMCID:PMC359338357)KAggarwal,LHChoeandKHLee(2006).
ShotgunproteomicsusingtheiTRAQisobarictags,BriefingsinFunctionalGenomicsandProteomics5:112-120.
PMID:1677227258)EJFinehout,ZFranck,NRRelkinandKHLee(2006).
ProteomicAnalysisofCerebrospinalFluidChangesRelatedtoPostmortemInterval,ClinicalChemistry52:1906-1913.
PMID:1688789959)DHLee,SGKim,YCPark,SWNam,KHLeeandJHSeo(2007).
ProteomeanalysisofrecombinantEscherichiacoliproducinghumanglucagon-likepeptide-1,JournalofChromatographyB849:323330.
60)EJFinehout,ZFranck,LHChoe,NRRelkinandKHLee(2007).
CerebrospinalFluidProteomicBiomarkersforAlzheimer'sDisease,AnnalsNeurology61:120-129.
PMID:1716778961)GuptaP,LeeKH(2007).
GenomicsandProteomicsinProcessDevelopment:OpportunitiesandChallenges,TrendsinBiotechnology25:324-330.
PMID:1747535362)KHLee,NRRelkin(2007).
Reply:CerebrospinalfluidbiomarkersforAlzheimer'sdisease,Annalsof1.
30.
20KelvinH.
LeePage6Neurology61:497-498.
63)RKuczenski,KHong,JGarcia-Ojalvo,andKHLee(2007).
PERIOD-TIMELESSintervaltimermayrequireanadditionalfeedbackloop,PLoSComputationalBiology3:1468-1476.
PMC1937016.
64)ChoeLH,D'AscenzoM,RelkinNR,PappinD,RossP,WilliamsonB,GuertinS,PribilP,LeeKH(2007).
8-PlexQuantitationofchangesincerebrospinalfluidproteinexpressioninsubjectsundergoingintravenousimmunoglobulintreatmentforAlzheimer'sdisease,Proteomics7:3651-3660.
PMCID:PMC359477765)LiaoPY,GuptaP,PetrovA,DinmanJ,LeeKH(2008).
AnewkineticmodelrevealsthesynergisticeffectofE-,P-andA-siteson+1ribosomalframeshifting,NucleicAcidsResearch36:2619-2629.
PMC2377451.
67)D'AscenzoM,ChoeLH,LeeKH(2008).
iTRAQPak:anRBasedAnalysisandVisualizationPackagefor8-plexIsobaricProteinExpressionData,BriefingsinFunctionalGenomicsandProteomics7:127135.
PMID:1827254768)GuptaP,LeeKH(2008).
SilentMutationsResultinHlyAHypersecretionbyReducingIntracellularHlyAProteinAggregates,BiotechnologyandBioengineering101:967-974.
PMID:1855350569)GriffithsL,ChoeLH,LeeKH,ReardonKF,OrtonE(2008).
ProteinExtractionandTwo-DimensionalGelElectrophoresisofWater-andLipid-SolubleProteinsFromBovinePericardium,aLow-CellularityTissue,Electrophoresis29:4508-4515.
PMC2791994.
70)ParkSM,LeeKH,CraigheadHC(2008).
On-chipcouplingofelectrochemicalpumpsandanSU-8tipforelectrosprayionizationmassspectrometry,BiomedicalMicrodevices10:891-897.
PMID:1856357071)RudrappaT,ChoiYS,LeviaDF,LegatesDR,LeeKH,BaisHP(2009).
Phragmitesaustralisrootsecretedphytotoxinundergoesphotodegradationtoexecuteseverephytotoxicity,PlantSignalingandBehavior4:1-8.
PMC2688296.
72)LiaoPY,ChoiYS,LeeKH(2009).
FSscan:amechanism-basedprogramtoidentify+1ribosomalframeshifthotspots.
,NucleicAcidsResearch37:7302-11.
PMC2790909.
73)LiaoPY,LeeKH(2010).
FromSNPstofunctionalpolymorphism:theinsightintobiotechnologicalapplications,BiochemicalEngineeringJournal49:149-158.
74)BechlerME,DoodyAM,RacoosinE,LinL,LeeKH,BrownWJ(2010).
ThephospholipasecomplexPAFAHIbregulatesthefunctionalorganizationoftheGolgicomplex,JournalofCellBiology190:45–53.
PMC291670.
75)ChoiY,ChoeLH,LeeKH(2010).
RecentcerebrospinalfluidbiomarkerstudiesofAlzheimer'sdisease,ExpertReviewofProteomics7:919-926.
PMID:2114289276)LiaoPY,ChoiYS,DinmanJ,LeeKH(2011).
Themanypathstoframeshifting:kineticmodellingandanalysisoftheeffectsofdifferentelongationstepsonprogrammed–1ribosomalframeshifting,NucleicAcidsResearch39:300-312.
PMC3017607.
77)ShayanG,ChoiY,ShustaEV,ShulerML,LeeKH(2011).
MurineInvitroModeloftheBlood-BrainBarrierforEvaluatingDrugTransport,EuropeanJournalofPharmaceuticalSciences42:148-55.
PMID:2107838678)HammondS,SwanbergJC,KaplarevicM,andLeeKH(2011).
GenomicsequencingandanalysisofaChineseHamsterovarycelllineusingIlluminasequencingtechnology,BMCGenomics12:67.
1.
30.
20KelvinH.
LeePage7PMC3038171.
79)WuCY,WhyeD,GlazewskiL,ChoeL,KerrD,LeeKH,MasonRW,WangW(2011).
ProteomicassessmentofacellmodelofSpinalMuscularAtrophy,BMCNeuroscience12:25.
PMC3063191.
80)ShayanG,ShulerML,LeeKH(2011).
TheEffectofAstrocytesontheInductionofBlood-BrainBarrierPropertiesinAorticEndothelialCells,BiotechnologyProgress27:1137-1145.
PMID:2162671981)XuX,NagarajanH,LewisNE,PanShengkai,LiuX,ChenW,XieM,WangW,HammondS,AndersenM,NeffN,PassarelliB,KohW,LeeKH,BetenbaughMJ,QuakeSR,FamiliI,PalssonBO,WangJ(2011).
TheGenomicSequenceoftheChineseHamsterOvary(CHO)K1CellLine,NatureBiotechnology29:735-741.
PMC3164356.
82)AggarwalK,Lee,KH(2011).
Over-expressionofclonedRhsAsequencesperturbsthecellulartranslationalmachineryinE.
coli,JournalofBacteriology193:4869-4880.
PMC3165670.
83)WuestD,HouS,LeeKH(2011).
MetabolicEngineering.
ComprehensiveBiotechnology.
ElsevierBV.
SecondEdition,2011,Vol.
3,617-628.
84)WuCY,CurtisA,ChoiY,MadaeM,XuMJ,BergA,JonejaU,MasonRW,LeeKH,WangW(2011).
IdentificationofthephosphorylationsitesinthesurvivalmotorneuronproteinbyproteinkinaseA,BiochmicaetBiophysicaActa1814:1134-1139.
PMC3124582.
85)Rakauskait,Rasa;Liao,Pei-Yu;Rhodin,Michael;Lee,Kelvin;Dinman,Jonathan(2011).
Arapid,inexpensiveyeast-baseddual-fluorescenceassayofprogrammed-1ribosomalframeshiftingforhighthroughputscreening,NucleicAcidsResearch39:e97.
PMC3152369.
86)HammondS,LeeKH(2011).
RNAInterferenceofCofilininChineseHamsterOvaryCellsImprovesRecombinantProteinProductivity,BiotechnologyandBioengineering109:528-535.
PMID:2191584887)WuestD,HarcumS,LeeKH(2011).
GenomicsinMammalianCellCultureBioprocessing,BiotechnologyAdvances30:629-638.
PMID:2207989388)MunroS,ChoeLH,ZinderS,LeeKH,WalkerL(2012).
ProteomicandPhysiologicalExperimentstoTestThermotoganeapolitanaConstraint-BasedModelHypothesesofCarbonSourceUtilization,BiotechnologyProgress28:312-318.
PMID:22034176.
89)WuestD,LeeKH(2012).
OptimizationofEndothelialCellGrowthinaMurineinvitroBlood-BrainBarrierModel,BiotechnologyJournal7:409-417.
PMCID:PMC348829690)HammondS,KaplarevicM,BorthN,BetenbaughM,LeeKH(2012).
ChineseHamsterGenomeDatabase:AnOnlineResourcefortheCHOCommunityatwww.
CHOgenome.
org,BiotechnologyandBioengineering109:1353-1356.
PMID:2210574491)HammondS,SwanbergJ,PolsonS,LeeKH(2012).
ProfilingConservedMicroRNAExpressioninRecombinantCHOCellLinesUsingIlluminaSequencing,BiotechnologyandBioengineering109:1371-1375.
PMID:22189905PMCID:PMC3330154.
92)ShayanG,AdamiakB,RelkinNR,LeeKH(2012).
LongitudinalAnalysisofNovelAlzheimer'sDiseaseProteomicCerebrospinalFluidBiomarkersDuringIntravenousImmunoglobulinTherapy.
Electrophoresis331975-1979.
PMID:2280646293)ValenteK,ChoeLH,LenhoffA,LeeKH(2012).
OptimizationofProteinSamplePreparationforTwo-DimensionalElectrophoresis.
Electrophoresis.
33:1947-1957.
PMID:22806459.
1.
30.
20KelvinH.
LeePage894)ShayanG,FelixN,ChoY,ChatzichristidiM,ShulerML,OberC,Lee,KH(2012).
SynthesisandCharacterizationofHigh-ThroughputNanofabricatedPoly(4-hydroxystyrene)MembranesforInvitroModelsofBarrierTissue.
TissueEngineeringPartC18:667-676.
PMID:22435738.
95)GuptaP,SwanbergJ,LeeKH(2012).
AsinglenucleotidepolymorphisminycdCalterstRNAsynthetaseexpressionandresultsinhypersecretioninEscherichiacoli,BiotechnologyProgress28:646-653.
PMID:22505047.
96)EspositoDV,ForestRV,ChangY,GaillardN,McCandlessBE,HouS,LeeKH,BirkmireRW,ChenJG(2012).
PhotoelectrochemicalReformingofGlucosetoProduceH2orSyngasusingaWO3-basedTandemCellDevice,EnergyandEnvironmentalScience5:9091-9099.
97)VillamilMA,LiangQ,ChenJ,ChoiYS,HouS,LeeKH,ZhuangZ(2012).
SerinephosphorylationiscriticalfortheactivationofUSP1anditsinteractionwithaWD40-repeatproteinUAF1.
Biochemistry.
51:9112-23.
PMCID:PMC361756198)HouS,JonesS,ChoeLH,PapoutsakisET,LeeKH(2013).
WorkflowforquantitativeproteomicanalysisofClostridiaacetobutylicumATCC824usingiTRAQtags.
METHODS61:269-76.
99)WuestDM,WingAM,LeeKH(2013).
MembraneConfigurationOptimizationforaMurineinvitroBlood-BrainBarrierModel.
J.
NeuroscienceMethods.
212:211-221.
PMID:23131353100)ChoiYC,HouS,ChoeLH,LeeKH(2013).
DevelopmentofatargetedproteomicsmethodforassayofAlzheimer'sdiseasecerebrospinalfluidbiomarkers,J.
Chrom.
B.
930:129-135.
PMID:23735279101)ValenteK,SchaeferAK,KemptonHR,LenhoffAM,LeeKH(2013)MethodtooptimizerecoveryofChinesehamsterovaryhostcellproteinsforproteomicanalysis.
BiotechnolJ.
9:87-99.
102)KremkowB,LeeKH(2013).
Next-generationSequencingTechnologiesandtheirPotentialImpactonCHOCell-basedBiomanufacturing.
PharmaceuticalBioprocessing.
1:455-465.
103)LevyN,ValenteK,ChoeLH,LeeKH,LenhoffA(2014).
IdentificationandCharacterizationofHostCellProteinProduct-AssociatedImpuritiesinMonoclonalAntibodyBioprocessing.
Biotechnol.
Bioeng.
111:904-912.
doi:10.
1002/bit.
25158.
PMID:24254318104)BaikJY,KHLee(2014).
miRNAExpressioninCHO:NatureKnowsBest.
BiotechnologyJ.
9:459460.
PMID:24519876doi:10.
1002/biot.
201300503105)Wuest,DM,KHLee(2014).
Amyloid-βconcentrationandstructureinfluencesthetransportandimmunomodulatoryeffectsofIVIG.
J.
Neurochem.
130:136-144.
doi:10.
1111/jnc.
12678PMID:24517534106)YukIM,ZhangJD,EbelingM,WerzS,GomezN,BerreraM,MeiringerC,ShaoZ,SwanbergJ,KHLee,LuoJ,andBSzperalski(2014).
EffectsofCopperonCHOCells:InsightsfromGeneExpressionAnalyses.
BiotechnologyProgress.
30:429-442.
DOI:10.
1002/btpr.
1868PMID:24403277107)TuerkA,KHLee(2014).
TheEvolvingEngineer.
AIChEJournal60:1956-1963.
108)ShayanG,AdamiakB,ChoeLH,RelkinNR,andKHLee(2014).
LongitudinalEffectsofIntravenousImmunoglobulinonAlzheimer'sCerebrospinalFluidProteome.
Electrophoresis35:1821-1827.
PMID:24756957109)BaikJY,andKHLee(2014).
TowardProductAttributeControl:DevelopmentsFromGenomeSequencing.
CurrentOpinioninBiotechnology.
30:40-44.
PMID:24874795110)KremkowBandKHLee(2015).
SequencingTechnologiesforAnimalCellCultureReseach.
1.
30.
20KelvinH.
LeePage9BiotechnologyLetters.
37:55-65.
111)ValenteK,LenhoffA,andKHLee(2015).
ExpressionofDifficult-to-RemoveHostCellProteinImpuritiesDuringExtendedChineseHamsterOvaryCellCultureandTheirImpactonContinuousBioprocessing.
Biotech.
Bioeng.
112(6),1232-1242.
112)MinL,ChoeLH,andKHLee(2015).
Improvedproteasedigestionconditionsformembraneproteindetection.
Electrophoresis.
36:1690-8.
doi:10.
1002/elps.
201400579.
PMID:2588427113)KremkowB,BaikJY,MacDonaldML,andKHLee(2015).
"CHOgenome.
org2.
0:Genomeresourcesandwebsiteupdates".
BiotechnologyJournal.
10:931-938.
114)VenkataramananKP,MinL,JonesSW,HouS,RalstonMR,LeeKHandETPapoutsakis(2015).
"Complexandextensivepost-transcriptionalregulationrevealedbyintegrativeproteomicandtranscriptomicanalysisofmetabolitestressresponseinClostridiumacetobutylicum".
BiotechnologyforBiofuelsBiology.
8:81.
doi:10.
1186/s13068-015-0260-9.
PMID:26269711PMCID:PMC4533764115)ChoiYS,LeeKH(2015).
MultiplereactionmonitoringassaybasedonconventionalliquidchromatographyandelectrosprayionizationforsimultaneousmonitoringofmultiplecerebrospinalfluidbiomarkercandidatesforAlzheimer'sdisease.
Arch.
Pharm.
Res.
doi10.
1007/s12272-015-0663y.
PMID:26404792116)LevyN,ValenteK,LeeKH,LenhoffA(2016).
"Hostcellproteinimpuritiesinchromatographicpolishingstepsformonoclonalantibodypurification".
BiotechnolBioeng.
113(6):1260-72.
doi:10.
1002/bit.
25882PMID:26550778117)HeintzKA,BregenzerME,MantleJL,LeeKH,WestJL,JHSlater(2016).
FabricationofThree-DimensionalBiomimeticMicrofluidicNetworksinHydrogels.
Adv.
Healthc.
Mater.
5:2153-2160.
10.
1002/adhm.
201600351.
PMID:27239785PMCID:PMC5014628.
118)Baik,JY,LeeKH(2016).
Towardimprovedhostcellproteinimpurityassessment.
BiotechnologyJ.
11:998-999.
doi:10.
1002/biot.
201600223.
PMID:27331371119)Levy,A,LeeKH,TEHanson(2016).
Chlorobaculumtepidummodulatesaminoacidcompositioninresponsetoenergyavailability,asrevealedbyasystematicexplorationoftheenergylandscapeofphototrophicsulfuroxidation.
ApplEnvironMicrobiol.
82:6431-6439.
PMID:27565613PMCID:PMC5066360120)MantleJ,MinL,LeeKH(2016).
MinimumTransendothelialElectricalResistanceThresholdsfortheStudyofSmallandLargeMoleculeDrugTransportinaHumaninVitroBlood-BrainBarrierModel.
MolecularPharmaceutics.
13:4191-4198.
PMID:27934481121)HarcumS,LeeKH(2016).
CHOCellsCanMakeMoreProtein.
CellSyst.
3:412-413.
PMID:27883886122)BaikJY,LeeKH(2017).
AframeworktoquantifykaryotypevariationassociatedwithCHOproductioninstability.
BiotechnolBioeng.
114:1045-1053.
PMID:27922175123)ChiuJ,ValenteKN,LevyNE,MinL,LenhoffAM,LeeKH(2017).
Knockoutofadifficult-to-removeCHOhostcellprotein,lipoproteinlipase,forimprovedpolysorbatestabilityinmonoclonalantibodyformulations.
BiotechnolBioeng.
114:1006-1015.
PMID:27943242PMCID:PMC5360534124)LertsuwanK,ChoeLH,MarwaI,Lee,KH,Sikes,R(2017).
IdentificationofFibulin-1asaHumanBoneMarrowStromal(HS-5)Cell-DerivedFactorthatInducesHumanProstateCancerCell.
Prostate.
Prostate.
77:729-742.
PMID:281687241.
30.
20KelvinH.
LeePage10125)LeeKH,SpringsS,CarbonellRG(2017).
NationalInstitutetoAccelerateInnovationinBiopharmaceuticalManufacturing.
BioProcessInternational.
15(9)i:12-16.
126)Rehmann,Matthew;Skeens,Kelsi;Kharkar,Prathamesh;Maverakis,Emanual;Lee,Kelvin;Kloxin,April(2017).
Tuningandpredictingmeshsizeandproteinreleasefromstepgrowthhydrogels.
Biomacromolecules18:3131-3142.
PMID:28850788127)ValenteK*,LevyN*,LeeKH,LenhoffAM(2018).
ApplicationsofProteomicMethodsforCHOHostCellProteinCharacterizationinBiopharmaceuticalManufacturing.
CurrentOpinioninBiotechnology2018,53:144–150.
PMID:29414072128)WeintraubWandLeeKH(2018).
AdvancesinCardiovascularCare:HowtoStimulateInnovationWhileControllingCost.
Accepted.
JACC:BasictoTranslationalScience.
3,114-118.
PMID:30062198129)BaikJY,LeeKH(2018).
GrowthratechangesinCHOhostcellsareassociatedwithkaryotypicheterogeneity.
BiotechnologyJournal.
13(3):e1700230.
doi:10.
1002/biot.
201700230.
PMID:28865132130)KremkowB,LeeKH(2018).
Glyco-Mapper:AChinesehamsterovary(CHO)genome-specificglycosylationpredictiontool.
MetabolicEngineering.
47:134-142.
doi:10.
1016/j.
ymben.
2018.
03.
002.
PMID:29522825131)Sawicki,L,ChoeL,WileyK,LeeKH,KloxinAM(2018).
Isolationandidentificationofproteinsecretedbycellsculturedwithinsynthetichydrogel-basedmatrices.
Accepted.
ACSBiomaterialsScience&Engineering.
4(3):836-845.
doi:10.
1021/acsbiomaterials.
7b00647.
PMID:29552635PMCID:PMC5850091132)GreeneDG,TraylorSJ,GuoJ,ChoeLH,ModlaS,XuX,SinghN,LockLL,GhoseS,LiZJ,LeeKH,WagnerNJ,LenhoffAM(2018).
MechanismsofprecipitateformationduringthepurificationofanFcfusionprotein.
BiotechnologyandBioengienering115:2489-2503.
doi:10.
1002/bit.
26746.
PMID:29896879PMCID:PMC6185765133)RuppO*,MacDonaldML*,LiS,DhimanH,GriepS,HeffnerK,HernandezI,BrinkrolfK,PolsonS,KinghamB,SamoudiM,BetenbaughM,GoesmannA,LewisNE,BorthN,LeeKH(2018).
AreferencegenomeoftheChinesehamsterbasedonahybridassemblystrategy.
BiotechnologyandBioengineering.
115:2087-2100.
DOI:10.
1002/bit.
26722.
PMID:29704459PMCID:PMC6045439.
134)MantleJL,Lee,KH(2018).
Adifferentiatingneuralstemcell-derivedastrocyticpopulationmitigatestheinflammatoryeffectsofTNF-αandIL-6inaniPSC-basedblood-brainbarriermodel.
NeurobiologyofDisease119:113-120.
PMID:30075293DOI:10.
1016/j.
nbd.
2018.
07.
030.
PMID:30075293135)MacDonaldM,HamakerN,LeeKH(2018).
BioinformaticanalysisofChinesehamsterovaryhostcellproteinlipases.
AIChEJournal.
64:4247-4254.
doi.
org/10.
1002/aic.
16378136)Ruano-SalgueroR,LeeKH(2018).
EffluxPumpSubstratesShuttledtoCytosolicorVesicularCompartmentsExhibitDifferentPermeabilityinaQuantitativeHumanBlood-BrainBarrierModel.
MolecularPharmaceutics.
15:5081-5088.
doi:10.
1021/acs.
molpharmaceut.
8b00662.
PMID:30212633137)DahodwalaH,LeeKH(2018).
TheFickleCHO:Areviewofthecauses,implicationsandpotentialalleviationoftheCHOcelllineinstabilityproblem.
CurrentOpinioninBiotechnology.
60:128-137.
doi:10.
1016/j.
copbio.
2019.
01.
011.
138)HamakerN,LeeKH(2018).
Site-specificIntegrationUshersinaNewEraofPreciseCHOCellLineEngineering.
Accepted.
CurrentOpinioninChemicalEngineering.
22:152-160.
doi:1.
30.
20KelvinH.
LeePage1110.
1016/j.
coche.
2018.
09.
011.
PMID:31086757PMCID:PMC6510493139)MantleJ,BakerJ,D'AmicoRW,KauffmanLR,RammohanJ,RogersK,RomantsevaE,StrychaslskiE,WelchJ,ShielJ,LeeKH.
Cyberbiosecurity-relatedconsiderationsinbiopharmaceuticalmanufacturing.
FrontiersinBioengineeringandBiotechnology.
7:116.
doi:10.
3389/fbioe.
2019.
00116140)MantleJL,LeeKH(2019).
ImmunoglobulinGtransportincreasesattheblood-brainbarrierwithamyloid-Bandwithneuroinflammatorycytokines.
BiotechnolBioeng.
2019116:1752-1761.
doi:10.
1002/bit.
26967.
PMID:30883675141)DeLeozMLA,DuewerDL,FungA,LiuL,YauHK,PotterO,StaplesGO,FurukiK,FrenkelR,HuY,SosicZ,ZhangP,AltmannF,GruNwald-GrubeC,ShaoC,ZaiaJ,EversW,PengelleyS,SuckauD,WiechmannA,ResemannA,JabsW,,BeckA,FroehlichJW,HuangC,LiY,LiuY,SunS,WangY,SeoY,AnHJ,ReichardtNC,RuizJE,Archer-HartmannS,AzadiP,BellL,LakosZ,AnY,CipolloJF,Pucic-BakovicM,tambukJ,LaucG,,LiX,WangPG,BockA,HennigR,RappE,,CreskeyM,CyrTD,NakanoM,SugiyamaT,LeungPA,Link-LenczowskiP,JaworekJ,YangS,ZhangH,KellyT,KlapoetkeS,CaoR,KimJY,LeeHK,LeeJY,YooJS,KimSR,SuhSK,deHaanN,FalckD,Lageveen-KammeijerGSM,WuhrerM,EmeryRJ,KozakRP,LiewLP,RoyleL,UrbanowiczPA,PackerNH,SongX,Everest-DassA,LattováE,CajicS,AlagesanK,KolarichD,KasaliT,LindoV,ChenY,GoswamiK,GauB,AmunugamaR,JonesR,StroopCJM,KatoK,,YagiH,KondoS,,YuenCT,HarazonoA,ShiX,MagnelliPE,KasperBT,MahalL,HarveyDJ,O'FlahertyR,RuddPM,SaldovaR,HechtES,MuddimanDC,KangJ,BhoskarP,MenardD,SaatiA,MerleC,MastS,TepS,TruongJ,NishikazeT,SekiyaS,ShaferA,FunaokaS,ToyodaM,deVreugdP,CaronC,PradhanP,TanNC,MechrefY,PatilS,RohrerJS,ChakrabartiR,DadkeD,LahoriM,ZouC,,CairoC,,ReizB,WhittalRM,LebrillaCB,WuL,GuttmanA,SzigetiM,KremkowBG,LeeKH,SihlbomC,AdamczykB,JinC,KarlssonNG,rnrosJ,LarsonG,NilssonJ,MeyerB,WiegandtA,KomatsuE,PerreaultH,BodnarED,,SaidN,FrancoisYN,Leize-WagnerE,MaierS,ZeckA,HeckAJR,YangY,HaselbergR,YuYQ,AlleyW,LeoneJW,YuanH,SteinSE.
(2020).
NISTInterlaboratoryStudyonGlycosylationAnalysisofMonoclonalAntibodies:ComparisonofResultsfromDiverseAnalyticalMethods.
MolecularandCellularProteomics.
19:11-30.
doi:10.
1074/mcp.
RA119.
001677.
PMID:31591262PMCID:PMC69442431.
30.
20