Levelkb936929

HVEMNF-kBLuciferaseReporterCellLineHVEMNF-kBLuciferaseReporterCellLineCatalog#C2034DescriptionHEK293TNF-kbreportercelllinestablyexpressingexogenoushumanHVEMgene.
RecombinantCellLineProleCatalog#:C2034Size:1Vial(1MCells)Species:HumanCellLineID:293T/hHVEM-luc2CellType:HumanembryonickidneyGeneID/Accession#:NM_003820.
3SelectionMarker:Puromycin(0.
5ug/ml)+Hygromycin(50ug/ml)Application:DrugscreeningandbiologicalassaysMorphology:FibroblastoidcellsgrowingadherentlyasmonolayerMedium:DMEM+10%FBSSubculture:Seedoutatca.
2-3x106cells/80cm2;splitconuentculture1:4-1:5every2-3daysIncubation:37°Cwith5%CO2storage:Frozeninliquidnitrogenwith70%medium+20%FBS+10%DMSODoublingTime:24-30hoursReporterGene:FireyluciferaseBiosafetyLevel:2References:Cell87:427-436(1996)CellLineCharacterizationHVEMNF-kBLuciferaseReporterCellLineCharacterizationofhumanHVEMoverexpressionin293TstableclonesbyFACS.
ResuscitationProtocolThawthevialassoonaspossibleuponreceipttoensureitsviability.
1.
Thawthefrozenvialina37°Cwaterbathfor1-2minutes.
2.
Transferthevialintobiosafetycabinet.
Wipethesurfacewith70%ethanolanddropthecellsuspensiongentlyintoacentrifugetubecontaining9.
0mLcompleteculturemedium.
3.
Spinat~125xg,for5-7minutesatroomtemperature.
4.
ResuspendcellpelletwiththeappropriatevolumeofcompletemediumandtransferthecellsuspensionintoaT25cultureask.
5.
Incubatethecultureat37°,5%CO2incubatororrefertothespecicculturecondition,ifpossible.
CellLineLimitedUseLicenseFORINVITROREASEARCHUSEONLY.
NOTFORTHERAPEUTICORDIAGNOSTICUSE.
BYACCEPTINGTHISPRODUCT,RECIPIENTAGREESTOBEBOUNDBYTHETERMSOFTHISLIMITEDUSESTATEMENT.
Thisproductmaynotberesoldortransferredbytherecipientandmaybeusedonlybytherecipientanditsaliate(asdenedbelow),intheirfacilityandonlyfor(1)researchuse,(2)drugdiscoveryanddevelopmentofbiologicdrugs,(3)qualityassurancetestingofbiologicdrugs,and(4)assaysforbiologicdrugs.
Nootheruse,especiallyanycommercialuse,isallowed.
"Commercialuse"meansanyandallusesofthisproductbyrecipientformonetaryorotherconsideration,includingprovidingaservice,supplyinginformationordatatounaliatedthirdparties,andresaleofthisproductforanyuse.
Recipienthasnorighttomodify,derivatize,geneticallyengineer,reverseengineer,duplicateorotherwisecreatevariationsoftheCells.
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ForthepurposeofthisdenitionofAliate,"control"meanstheownershipandvotingcontrolofmorethanftypercent(50%)oftheoutstandingvotingsecuritiesorinterestincapitalorprotsofanentity,ortherighttodirectorcontrolthemanagementoraairsofsuchentitybycontractorsimilararrangement.
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