hspBnod32

TableS1Genesandopenreadingframes(ORFs)whoseexpressionisaffectedbythenifAnullmutationinB.
japonicumstrainA9Gene/ORFKazusaannotations1ProteindescriptionAveragefoldchangeStdDevDecreasedexpressioninnifAmutantcomparedtowildtypenifDblr1743Nitrogenasemolybdenum-ironproteinalphachain-21.
1911.
63nrgAlN-acetyltransferasehomolog-18.
280.
89fdxNbsr1739Ferredoxin-11.
442.
18id525naOutermembraneprotein-10.
351.
08nifKblr1744Nitrogenasemolybdenum-ironproteinbetachain-6.
505.
63nifHblr1769Nitrogenaseironprotein-5.
262.
41exoU2l,sUDP-hexosetransferase-5.
210.
89papAl,sPutativealkalineprotease-4.
711.
29id903blr2098Desthiobiotinsynthase-4.
714.
45id899blr2096Aspartate1-decarboxylase-4.
221.
53id407blr1896TranscriptionalregulatoryproteinFisfamily-3.
672.
08exoPsSimilartosuccinoglycanbiosynthesistransportprotein-3.
461.
72id904blr2099Adenosylmethionine-8-amino-7-oxononanoateaminotransferase-3.
291.
13id897blr2095Biotinsynthetase-3.
200.
92id89bll1754Unknownprotein-3.
031.
99nifSblr1756Cysteinedesulfurase-2.
931.
48Q9RHC2l,sUnknownprotein-2.
900.
23id901blr20978-amino-7-oxononanoatesynthase-2.
880.
94id91blr1755Unknownprotein-2.
691.
94id590naUnknownprotein-2.
650.
33ahpCbll1777Alkylhydroperoxidereductase-2.
651.
01id117bll1766Outermembraneprotein-2.
470.
29id485lUnknownprotein-2.
470.
17Q8RLG0l,sUnknownprotein-2.
360.
18id921naUnknownprotein-2.
340.
60galFl,sNucleotidyltransferasefamilyprotein-2.
310.
66hmuRlHeminreceptorprecursor-2.
250.
60nodWbll1714Two-componentregulatorysystemresponseregulator-2.
100.
08Q93Q03sAcetyl-coenzymeAsynthetase-2.
070.
37tonBbll7071Periplasmicenergytransducerprotein-2.
060.
29id460blr1916Putativetransposase-2.
050.
22id328bll1862Unknownprotein-2.
010.
28Q9RHC3bll7571ExoQ-likeprotein-2.
010.
28IncreasedexpressioninnifAmutantcomparedtowildtypeaphII-Aminoglycoside3'-phosphotransferase208.
1057.
72hspCblr5234Smallheatshockprotein11.
306.
48degPblr5235Serineprotease8.
671.
51groES1blr522610kDachaperonin17.
703.
19hspBblr5233Smallheatshockprotein7.
093.
72hspEblr5220Smallheatshockprotein5.
375.
16groEL1blr522760kDachaperonin15.
341.
02cycOblr2696Cytochromecperoxidase4.
212.
19O69244blr5222Unknownprotein3.
871.
96hspFblr5221Smallheatshockprotein3.
541.
16hspHblr6571Smallheatshockprotein3.
390.
83hspDbll5219Smallheatshockprotein3.
390.
83O52842l,sUnknownprotein3.
061.
33id684naUnknownprotein2.
771.
08O52841bll5661Unknownprotein2.
730.
48acnAbll0466Aconitatehydratase2.
670.
82O52843l,sUnknownprotein2.
360.
65rpoH1blr5231Sigma-32-liketranscriptionfactor2.
220.
39groEL2blr697960kDachaperonin22.
190.
57regRlTwo-componentregulatorysystemresponseregulator2.
150.
15nrdl,sSimilartonitrilotriacetatemonooxygenasecomponentA2.
150.
15Q9REE9lUnknownprotein2.
110.
31O52844lUnknownprotein2.
080.
49Q45226l,sUnknownprotein2.
050.
161TheKazusaannotationislistedwherepossible.
Inalltheothercaseseitherthelength(l)and/orthesequence(s)ofthecodingsequenceweredifferenttothepreviousannotation.
Insomecasesgeneswerenotannotatedandarethereforemarkedasna.
2Thechangep-valueforexoUwas0.
94inoneofthethreereplicates,thusslightlylowerthanthe0.
98thresholdlevelwhichisvalidforallothergenes.
TableS2Genesandopenreadingframes(ORFs)whoseexpressionisaffectedbytheregRnullmutationinstrain2426Gene/ORFKazusaannotation1ProteindescriptionAveragefoldchangeStdDevDecreasedexpressioninregRmutantcomparedtowildtyperegRlTwo-componentregulatorysystemresponseregulator-46.
458.
38id880bll2087Unknownprotein-25.
507.
62fixRblr2036Oxidoreductase-12.
814.
08id937lCRP-/FNR-typetranscriptionalregulator-11.
774.
68nodD2bll2021LysR-typetranscriptionalregulator-3.
702.
79Q8RLG0l,sUnknownprotein-3.
480.
60nifAblr2037Nif-specifictranscriptionalregulator-3.
040.
21id156bll2159Putativetransposase-2.
800.
21id199bll1804Unknownprotein-2.
780.
64id362bll1877Unknownprotein-2.
670.
85id158bsr1784Unknownprotein-2.
200.
37fixK2lCRP-/FNR-typetranscriptionalregulator-2.
070.
47fixAblr2038Electrontransferflavoproteinbetachain-1.
970.
34IncreasedexpressioninregRmutantcomparedtowildtypeaadA-aminoglycoside-3″-adenylyltransferaseFDVVHWWH1016353.
70hspEblr5220Smallheatshockprotein11.
635.
37hspBblr5233Smallheatshockprotein8.
303.
82groES1blr522610kDachaperonin13.
610.
52hspCblr5234Smallheatshockprotein3.
500.
78hspDbll5219Smallheatshockprotein3.
200.
55nrdl,sSimilartonitrilotriacetatemonooxygenasecomponentA3.
000.
31degPblr5235Serineprotease2.
980.
48dnaJblr0680Co-chaperone2.
430.
14groEL1blr522760kDachaperonin12.
130.
31Q45226l,sUnknownprotein2.
000.
101TheKazusaannotationislistedwherepossible.
Inalltheothercaseseitherthelength(l)and/orthesequence(s)ofthecodingsequenceweredifferenttothepreviousannotation.
TableS3Genesandopenreadingframes(ORFs)whoseexpressionisaffectedbythecellularoxygenconditionsGene/ORFKazusaannotations1ProteindescriptionAveragefoldchangeStdDevIncreasedexpressioninmicroaerobicallygrowncellscomparedtoaerobicallygrowncellsfixOblr2764CytochromecoxidasesubunitII151.
1453.
99fixNblr2763CytochromecoxidasesubunitI47.
737.
08napEsPeriplasmicnitratereductaseprotein23.
1114.
14fixQlCytochromecoxidasesubunitIV21.
545.
47fixGblr2767Cytochromecoxidasematurationprotein21.
196.
07hemN1lCoproporphyrinogenIIIdehydrogenase20.
671.
69napCsPeriplasmicnitratereductase-associatedc-typecytochrome17.
343.
02cycBblr6128Cytochromec55214.
623.
92fixSbsr2770Cytochromecoxidasematurationprotein13.
454.
42fixK1bll6061CRP-/FNR-typetranscriptionalregulator13.
375.
49fixPblr2766Cytochromecoxidase,subunitIII13.
106.
17napAl,sPeriplasmicnitratereductaselargesubunit11.
442.
18id838naUnknownprotein11.
303.
78hemBblr5037Delta-aminolevulinicaciddehydratase10.
576.
57hemN2bll7086Oxygen-independentcoproporphyrinogenIIIoxidase9.
501.
68fixK2lCRP-/FNR-typetranscriptionalregulator9.
471.
32id841blr2071Similartoinosamine-phosphateamidinotransferase9.
083.
70fixIblr2769Cytochromecoxidasematurationprotein8.
294.
89nifDblr1743Nitrogenasemolybdenum-ironproteinalphachain8.
137.
87id835naUnknownprotein7.
691.
07id833lUnknownprotein7.
615.
60Q03082blr2761Unknownprotein7.
262.
38fixHblr2768Cytochromecoxidasematurationprotein7.
071.
46Q52766blr2762Unknownprotein6.
941.
65napBl,sPeriplasmicnitratereductasesmallsubunit6.
301.
28nirKl,sRespiratorynitritereductase6.
081.
74id117bll1766Outermembraneprotein5.
970.
88Q99333bll2758Unknownprotein5.
832.
42pcdAl,sProtocatechuate-3,4-dioxygenasealphasubunit5.
742.
98napDblr7037Periplasmicnitratereductasematurationprotein5.
072.
08pcdBl,sProtocatechuate-3,4-dioxygenasebetasubunit4.
390.
18nifHblr1769Nitrogenaseironprotein4.
363.
67nifKblr1744Nitrogenasemolybdenum-ironproteinbetachain4.
072.
94Q93SI1bll7083Unknownprotein3.
380.
79hemAbll12005-Aminolevulinicacidsynthase3.
260.
38id526lUnknownprotein2.
811.
28ahpCbll1777Alkylhydroperoxidereductase2.
810.
59P29285bll6063Unknownprotein2.
720.
45nosRNitrousoxidereductaseexpressionregulator2.
541.
27id91blr1755Unknownprotein2.
100.
82rpoN1lRNApolymerasesigma-54factor12.
080.
49nnrRblr7084CRP-/FNR-typetranscriptionalregulator1.
960.
16Decreasedexpressioninmicroaerobicallygrowncellscomparedtoaerobicallygrowncellsid199bll1804Unknownprotein-15.
3412.
62id274bll1840Unknownprotein-7.
901.
35id855blr2074Fucose-specificsulfotransferase(NoeEhomolog)-7.
574.
58Q8RLG0l,sUnknownprotein-6.
360.
52nolBlNodulationprotein-5.
190.
77nolUblr1814Nodulationprotein-5.
113.
65mtbAbll1193Integralinnermembranemetabolitetransportprotein-4.
521.
64id637blr1994Putativepectinesterase-4.
301.
15rhcRblr1819ComponentoftypeIIIsecretionsystem-4.
220.
64rhcC2lComponentoftypeIIIsecretionsystem-4.
152.
01id252bsr1831Unknownprotein-3.
851.
15id850naUnknownprotein-3.
850.
64nodD2bll2021LysR-typetranscriptionalregulator-3.
550.
90id891bll2091Para-aminobenzoatesynthasecomponentII-3.
500.
48gunAlPutativeglycosylhydrolase-3.
461.
58id636blr1993Putativepolygalacturonase-3.
140.
59id893bll2092Para-aminobenzoatesynthasecomponentI-3.
050.
35id863blr2077Dihydrorhizobitoxinesynthasehomolog-2.
981.
45id362bll1877Unknownprotein-2.
790.
88id195bll1803Unknownprotein-2.
730.
90id205blr1806Unknownprotein-2.
670.
51fixRblr2036Oxidoreductase-2.
610.
78id202bll1805Unknownprotein-2.
580.
10id204naUnknownprotein-2.
440.
51id912blr2102Pantoate-beta-alanineligase-2.
430.
37id342lUnknownprotein-2.
410.
10O06003l,sUnknownprotein-2.
360.
18id158bsr1784Unknownprotein-2.
310.
29atpBl,sF0F1-ATPsynthasesubunitB-2.
310.
29id211lUnknownprotein-2.
300.
16ndvCl,sPutativebeta(1-6)-glucansynthase-2.
250.
18id397blr1889ABCtransportersubstrate-bindingprotein-2.
200.
60id405blr1894Putativephosphotransferase-2.
200.
81id485lUnknownprotein-2.
190.
52scdh1bll0450Putativeshort-chainfattyaciddehydrogenase-2.
170.
38glnIIl,sGlutaminesynthetaseII-2.
150.
15id662bsr1999Unknownprotein-2.
090.
51cycAblr7544Cytochromec550-2.
050.
08shcblr3004Squalene-hopenecyclase-2.
020.
39id487naUnknownprotein-2.
020.
61id299blr1847VirKproteinhomolog-1.
970.
341TheKazusaannotationislistedwherepossible.
Inalltheothercaseseitherthelength(l)and/orthesequence(s)ofthecodingsequenceweredifferenttothepreviousannotation.
Insomecasesgeneswerenotannotatedandarethereforemarkedasna.