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MethodsinMolecularBiologySeriesEditorJohnM.
WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForfurthervolumes:http://www.
springer.
com/series/7651wwwwwwwwwwwwwwwPermeabilityBarrierMethodsandProtocolsEditedbyKursadTurksenRegenerativeMedicineProgram,SprottCentreforStemCellResearch,TheOttawaHospitalResearchInstitute,Ottawa,ON,CanadaEditorKursadTurksen,Ph.
DRegenerativeMedicineProgramSprottCentreforStemCellResearchTheOttawaHospitalResearchInstituteOttawa,ONCanadakturksen@ohri.
caPleasenotethatadditionalmaterialforthisbookcanbedownloadedfromhttp://extras.
springer.
comISSN1064-3745e-ISSN1940-6029ISBN978-1-61779-190-1e-ISBN978-1-61779-191-8DOI10.
1007/978-1-61779-191-8SpringerNewYorkDordrechtHeidelbergLondonLibraryofCongressControlNumber:2011935456SpringerScience+BusinessMedia,LLC2011Allrightsreserved.
Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.
Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.
Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.
Printedonacid-freepaperHumanaPressispartofSpringerScience+BusinessMedia(www.
springer.
com)vPrefaceWhenIapproachedDr.
JohnWalker,theEditor-in-ChiefoftheMethodsinMolecularBiologyseries,withtheideaofputtingtogetheraprotocolvolumeonthepermeabilitybarrier,hisimmediateresponsewas"Whatagreatidea.
Wehavenotcoveredthistopic!
"John:Thanksforthegreatsupportforthisvolumefromthebeginning.
Althoughthetopicofthepermeabilitybarrierisdiverse,theauthorshereincovermanycriticalareas,makingthisvolumevaluabletoalmostanybodywhoisinterestedinthisfield.
Ihopethatthemanyvaluableprotocolsincludedwillbeusefulforbothnovicesandexpertsalike.
Ithankallthecontributorsforverygraciouslyprovidingtheirprotocolsforthisvolume.
Withoutthemandtheirwillingnesstoshareprotocoldetails,thisnewvolumewouldnothavematerialized.
PatrickMarton,theEditoroftheMethodsinMolecularBiologyseriesatSpringer,hasbeenagreatsupportandisalwaysavailabletoanswermyquestionsandlistentomysuggestions.
AveryspecialthanksgoestoDavidCaseyforhisinvaluablehelpduringtheproductionstagesofthisvolume.
Ottawa,Ontario,CanadaKursadTurksenwwwwwwwwwwwwwwwviiContentsPrefacevContributorsxi1ComputationalModelingoftheSkinBarrier1ArneNaegel,MichaelHeisig,andGabrielWittum2InVitroHumanSkinSegmentationandDrugConcentration–SkinDepthProfiles33AnaMelero,TsambikaHahn,UlrichF.
Schaefer,andMarcSchneider3TranscriptionalRegulationofEpidermalBarrierFormation51AmbicaBhandari,MichaelL.
Salmans,WilliamGordon,andBogiAndersen4EpidermalPermeabilityBarrierMeasurementinMammalianSkin73ArupKumarIndraandMarkLeid5AssessmentofPermeabilityBarrierstoMacromoleculesintheRodentEndometriumattheOnsetofImplantation83BrentM.
BanyandG.
ScotHamilton6AssessmentofIntestinalPermeabilityin(Premature)NeonatesbySugarAbsorptionTests95WillemijnE.
Corpeleijn,RuurdM.
vanElburg,IdoP.
Kema,andJohannesB.
vanGoudoever7AnalysisofEpithelialCellSheddingandGapsintheIntestinalEpithelium105CarrieA.
DuckworthandAlastairJ.
Watson8StudyingPermeabilityinaCommonlyUsedEpithelialCellLine:T84IntestinalEpithelialCells115RinoP.
Donato,AdaweyahEl-Merhibi,BatjargalGundsambuu,KaiYanMak,EmmaR.
Formosa,XianWang,CatherineA.
Abbott,andBarryC.
Powell9OptimizationoftheCaco-2PermeabilityAssaytoScreenDrugCompoundsforIntestinalAbsorptionandEfflux139BarryPress10OuabainModulatesCellContactsaswellasFunctionsthatDependonCellAdhesion155IsabelLarre,RubenG.
Contreras,andMarcelinoCereijido11MonitoringoftheDynamicsofEpithelialDomeFormationUsingaNovelCultureChamberforLong-TermContinuousLive-CellImaging169JudithLechner,DanielHekl,HelmutGatt,MarkusVoelp,andThomasSeppiviiiContents12MeasuringPermeabilityinHumanRetinalEpithelialCells(ARPE-19):ImplicationsfortheStudyofDiabeticRetinopathy179MartaGarcia-Ramírez,MartaVillarroel,LídiaCorraliza,CristinaHernández,andRafaelSimó13AnalysisofEpithelialBarrierIntegrityinPolarizedLungEpithelialCells195MonikaStrengertandUllaG.
Knaus14PermeabilityofDifferentiatedHumanUrotheliumInVitro207PeterRubenwolfandJenniferSouthgate15PhenotypingtheClaudin11DeficiencyinTestis:FromHistologytoImmunohistochemistry223SéverineMazaud-Guittot,AlexanderGow,andBrigitteLeMagueresse-Battistoni16AnInVitroSystemtoStudySertoliCellBlood-TestisBarrierDynamics237DoloresD.
MrukandC.
YanCheng17AnalysisofEndothelialBarrierFunctionInVitro253YupingWangandJ.
StevenAlexander18RoleofEndothelialCell–CellJunctionsinEndothelialPermeability265ArmelleLeGuelteandJulieGavard19InVitroAnalysesofEndothelialCellPermeability281ElizabethMonaghan-BensonandErikaS.
Wittchen20Mechano-TransductionandBarrierRegulationinLungMicrovascularEndothelialCells291KristinaGiantsos,MarkCluff,andRandalDull21RoleofCaveolin-1intheRegulationofPulmonaryEndothelialPermeability303YuSun,RichardD.
Minshall,andGuochangHu22AssessmentofEndothelialPermeabilityandLeukocyteTransmigrationinHumanEndothelialCellMonolayers319AndreasLudwig,AnselmSommer,andStefanUhlig23PermeabilityofEndothelialBarrier:CellCultureandInVivoModels333AlexanderN.
Garcia,StephenM.
Vogel,YuliaA.
Komarova,andAsrarB.
Malik24Size-SelectiveandInVitroAssessmentofInnerBloodRetinaBarrierPermeability355MatthewCampbellandPeterHumphries25AssessmentofPermeabilityinBarrierTypeofEndotheliuminBrainUsingTracers:EvansBlue,SodiumFluorescein,andHorseradishPeroxidase369MehmetKayaandBulentAhishali26InVitroandInVivoMethodsforAssessingFcRn-MediatedReverseTranscytosisAcrosstheBlood–BrainBarrier383NadiaCaram-Salas,EveBoileau,GrahamK.
Farrington,EllenGarber,EricBrunette,AbedelnasserAbulrob,andDanicaStanimirovic27EvaluationofVEGF-InducedVascularPermeabilityinMice403SaraM.
WeisixContents28InVivoMeasurementofGlioma-InducedVascularPermeability417JisookLee,AndrewBaird,andBrianP.
Eliceiri29InVivoOpticalImagingofIschemicBlood–BrainBarrierDisruption423AbedelnasserAbulrob,EricBrunette,JacquelineSlinn,EwaBaumann,andDanicaStanimirovicIndex441wwwwwwwwwwwwwwwxiContributorsCatherineA.
AbbottSchoolofBiologicalSciences,FlindersUniversity,Adelaide,SA,AustraliaAbedelnasserAbulrobInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaJ.
StevenAlexanderDepartmentofMolecularandCellularPhysiology,LouisianaStateUniversityHealthSciencesCenter,Shreveport,LA,USABulentAhishaliDepartmentsofPhysiologyandHistologyandEmbryology,IstanbulFacultyofMedicine,IstanbulUniversity,Istanbul,TurkeyBogiAndersenDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAAndrewBairdDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USABrentM.
BanyDepartmentofPhysiologyandObstetricsandGynecology,SouthernIllinoisUniversitySchoolofMedicine,Carbondale,IL,USAEwaBaumannCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAmbicaBhandariDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAEveBoileauInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaEricBrunetteCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMatthewCampbellUCDSchoolofBiomolecularBiomedicalSciences,ConwayInstitute,UniversityCollegeDublin,Dublin,IrelandNadiaCaram-SalasInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMarcelinoCereijidoDepartmentofPhysiology,BiophysicsandNeurosciences,CenterforResearch&AdvancedStudies(CINVESTAV),Av.
InstitutoPolitécnicoNacional2508,Col.
SanPedroZacatenco,Del.
GAMC.
P.
07360MéxicoC.
YanChengCenterforBiomedicalResearch,PopulationCouncil,NewYork,NY,USAMarkCluffDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USARubenG.
ContrerasCenterforResearch&AdvancedStudies(CINVESTAV),MexicoCity,MéxicoWillemijnE.
CorpeleijnDivisionofNeonatology,ErasmusMC–SophiaChildren'sHospital,Rotterdam,TheNetherlandsxiiContributorsLídiaCorralizaDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainRinoP.
DonatoWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaCarrieA.
DuckworthGastroenterologyResearchUnit,SchoolofClinicalSciences,UniversityofLiverpool,Liverpool,UKRandalDullDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USARuurdM.
vanElburgDepartmentofPediatrics,DivisionofNeonatology,VrijeUniversiteitMedicalCenter,Amsterdam,TheNetherlandsBrianP.
EliceiriDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USAAdaweyahEl-MerhibiaWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaGrahamK.
FarringtonInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaEmmaR.
FormosaWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaEllenGarberInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAlexanderN.
GarciaDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAMartaGarcia-RamírezDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainHelmutGattDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaJulieGavardInstitutCochin,UniversitéParisDescartes,UMR-CNRS8104,Paris,FranceKristinaGiantsosDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USAWilliamGordonDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAJohannesB.
vanGoudoeverDivisionofNeonatology,ErasmusMC–SophiaChildren'sHospital,Rotterdam,TheNetherlandsAlexanderGowCenterforMolecularMedicineandGenetics,WayneStateUniversity,Detroit,MI,USAArmelleLeGuelteInstitutCochin,UniversitéParisDescartes,UMR-CNRS8104,Paris,FranceBatjargalGundsambuuAWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaTsambikaHahnBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyxiiiContributorsG.
ScotHamiltonDepartmentofPhysiologyandPharmacology,UniversityofWesternOntario,London,ON,CanadaDanielHeklDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaMichaelHeisigGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,GermanyCristinaHernándezDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelona,Barcelona,SpainGuochangHuDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAPeterHumphriesUCDSchoolofBiomolecularBiomedicalSciences,ConwayInstitute,UniversityCollegeDublin,Dublin,IrelandArupKumarIndraDepartmentofPharmaceuticalSciences,CollegeofPharmacy,andEnvironmentalHealthSciencesCenter,OregonStateUniversity,Corvallis,OR,USAMehmetKayaDepartmentsofPhysiology&HistologyandEmbryology,IstanbulFacultyofMedicine,IstanbulUniversity,Istanbul,TurkeyIdoP.
KemaDepartmentofLaboratoryMedicine,DivisionofClinicalChemistryandHematology,UniversityMedicalCenterGroningen,Groningen,TheNetherlandsUllaG.
KnausConwayInstitute,UniversityCollegeDublin,Dublin,IrelandYuliaA.
KomarovaDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAIsabelLarreCenterforResearch&AdvancedStudies(CINVESTAV),MexicoCity,MéxicoJudithLechnerDivisionofPhysiology,DepartmentofPhysiologyandMedicalPhysics,DivisionofPhysiology,InnsbruckMedicalUniversity,Innsbruck,AustriaJisookLeeDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USAMarkLeidDepartmentofPharmaceuticalSciences,CollegeofPharmacy,andEnvironmentalHealthSciencesCenter,OregonStateUniversity,Corvallis,OR,USAAndreasLudwigInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyBrigitteLeMagueresse-BattistoniInsermU870,Oullins,Oullins,France;2INRA,UMR1235,Oullins,France;3INSA-Lyon,RMND,Villeurbanne,FranceKaiYanMakaAWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaAsrarB.
MalikDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USASéverineMazaud-GuittotInsermU870,Oullins,France,2INRA,UMR1235,Oullins,France;3INSA-Lyon,RMND,Villeurbanne,FranceAnaMeleroBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyxivContributorsRichardD.
MinshallDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAElizabethMonaghan-BensonDepartmentofCellandDevelopmentalBiologyandLinebergerCancerCenter,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC,USADoloresD.
MrukCenterforBiomedicalResearch,PopulationCouncil,NewYork,NY,USAArneNaegelGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,GermanyBarryC.
PowellWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaBarryPressMaRSCentre,OntarioInstituteforCancerResearch,Toronto,ON,CanadaPeterRubenwolfDepartmentofPaediatricUrology,RegensburgUniversityHospital,KlinikSt.
HedwigRegensburg,GermanyMichaelL.
SalmansDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAUlrichF.
SchaeferBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyMarcSchneiderPharmaceuticalNanotechnology,SaarlandUniversity,Saarbrucken,GermanyThomasSeppiDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaRafaelSimóDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainJacquelineSlinnCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAnselmSommerInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyJenniferSouthgateJackBirchUnitofMolecularCarcinogenesis,DepartmentofBiology,UniversityofYork,York,UKDanicaStanimirovicCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMonikaStrengertConwayInstitute,UniversityCollegeDublin,Dublin,IrelandYuSunDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAStefanUhligInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyMartaVillarroelDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainxvContributorsMarkusVoelpInnovativeTechnologie-Voelp(IT-V),Innsbruck,AustriaStephenM.
VogelDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAXianWangWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaYupingWangDepartmentofObstetricsandGynecology,LouisianaStateUniversityHealthSciencesCenter,Shreveport,LA,USAAlastairJ.
WatsonGastroenterologyResearchUnit,SchoolofClinicalSciences,UniversityofLiverpool,Liverpool,UKSaraM.
WeisMooresUCSDCancerCenter,UniversityofCalifornia,SanDiego,LaJolla,CA,USAErikaS.
WittchenDepartmentofCellandDevelopmentalBiologyandLinebergerCancerCenter,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC,USAGabrielWittumGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,Germanywwwwwwwwwwwww
WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForfurthervolumes:http://www.
springer.
com/series/7651wwwwwwwwwwwwwwwPermeabilityBarrierMethodsandProtocolsEditedbyKursadTurksenRegenerativeMedicineProgram,SprottCentreforStemCellResearch,TheOttawaHospitalResearchInstitute,Ottawa,ON,CanadaEditorKursadTurksen,Ph.
DRegenerativeMedicineProgramSprottCentreforStemCellResearchTheOttawaHospitalResearchInstituteOttawa,ONCanadakturksen@ohri.
caPleasenotethatadditionalmaterialforthisbookcanbedownloadedfromhttp://extras.
springer.
comISSN1064-3745e-ISSN1940-6029ISBN978-1-61779-190-1e-ISBN978-1-61779-191-8DOI10.
1007/978-1-61779-191-8SpringerNewYorkDordrechtHeidelbergLondonLibraryofCongressControlNumber:2011935456SpringerScience+BusinessMedia,LLC2011Allrightsreserved.
Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.
Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.
Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.
Printedonacid-freepaperHumanaPressispartofSpringerScience+BusinessMedia(www.
springer.
com)vPrefaceWhenIapproachedDr.
JohnWalker,theEditor-in-ChiefoftheMethodsinMolecularBiologyseries,withtheideaofputtingtogetheraprotocolvolumeonthepermeabilitybarrier,hisimmediateresponsewas"Whatagreatidea.
Wehavenotcoveredthistopic!
"John:Thanksforthegreatsupportforthisvolumefromthebeginning.
Althoughthetopicofthepermeabilitybarrierisdiverse,theauthorshereincovermanycriticalareas,makingthisvolumevaluabletoalmostanybodywhoisinterestedinthisfield.
Ihopethatthemanyvaluableprotocolsincludedwillbeusefulforbothnovicesandexpertsalike.
Ithankallthecontributorsforverygraciouslyprovidingtheirprotocolsforthisvolume.
Withoutthemandtheirwillingnesstoshareprotocoldetails,thisnewvolumewouldnothavematerialized.
PatrickMarton,theEditoroftheMethodsinMolecularBiologyseriesatSpringer,hasbeenagreatsupportandisalwaysavailabletoanswermyquestionsandlistentomysuggestions.
AveryspecialthanksgoestoDavidCaseyforhisinvaluablehelpduringtheproductionstagesofthisvolume.
Ottawa,Ontario,CanadaKursadTurksenwwwwwwwwwwwwwwwviiContentsPrefacevContributorsxi1ComputationalModelingoftheSkinBarrier1ArneNaegel,MichaelHeisig,andGabrielWittum2InVitroHumanSkinSegmentationandDrugConcentration–SkinDepthProfiles33AnaMelero,TsambikaHahn,UlrichF.
Schaefer,andMarcSchneider3TranscriptionalRegulationofEpidermalBarrierFormation51AmbicaBhandari,MichaelL.
Salmans,WilliamGordon,andBogiAndersen4EpidermalPermeabilityBarrierMeasurementinMammalianSkin73ArupKumarIndraandMarkLeid5AssessmentofPermeabilityBarrierstoMacromoleculesintheRodentEndometriumattheOnsetofImplantation83BrentM.
BanyandG.
ScotHamilton6AssessmentofIntestinalPermeabilityin(Premature)NeonatesbySugarAbsorptionTests95WillemijnE.
Corpeleijn,RuurdM.
vanElburg,IdoP.
Kema,andJohannesB.
vanGoudoever7AnalysisofEpithelialCellSheddingandGapsintheIntestinalEpithelium105CarrieA.
DuckworthandAlastairJ.
Watson8StudyingPermeabilityinaCommonlyUsedEpithelialCellLine:T84IntestinalEpithelialCells115RinoP.
Donato,AdaweyahEl-Merhibi,BatjargalGundsambuu,KaiYanMak,EmmaR.
Formosa,XianWang,CatherineA.
Abbott,andBarryC.
Powell9OptimizationoftheCaco-2PermeabilityAssaytoScreenDrugCompoundsforIntestinalAbsorptionandEfflux139BarryPress10OuabainModulatesCellContactsaswellasFunctionsthatDependonCellAdhesion155IsabelLarre,RubenG.
Contreras,andMarcelinoCereijido11MonitoringoftheDynamicsofEpithelialDomeFormationUsingaNovelCultureChamberforLong-TermContinuousLive-CellImaging169JudithLechner,DanielHekl,HelmutGatt,MarkusVoelp,andThomasSeppiviiiContents12MeasuringPermeabilityinHumanRetinalEpithelialCells(ARPE-19):ImplicationsfortheStudyofDiabeticRetinopathy179MartaGarcia-Ramírez,MartaVillarroel,LídiaCorraliza,CristinaHernández,andRafaelSimó13AnalysisofEpithelialBarrierIntegrityinPolarizedLungEpithelialCells195MonikaStrengertandUllaG.
Knaus14PermeabilityofDifferentiatedHumanUrotheliumInVitro207PeterRubenwolfandJenniferSouthgate15PhenotypingtheClaudin11DeficiencyinTestis:FromHistologytoImmunohistochemistry223SéverineMazaud-Guittot,AlexanderGow,andBrigitteLeMagueresse-Battistoni16AnInVitroSystemtoStudySertoliCellBlood-TestisBarrierDynamics237DoloresD.
MrukandC.
YanCheng17AnalysisofEndothelialBarrierFunctionInVitro253YupingWangandJ.
StevenAlexander18RoleofEndothelialCell–CellJunctionsinEndothelialPermeability265ArmelleLeGuelteandJulieGavard19InVitroAnalysesofEndothelialCellPermeability281ElizabethMonaghan-BensonandErikaS.
Wittchen20Mechano-TransductionandBarrierRegulationinLungMicrovascularEndothelialCells291KristinaGiantsos,MarkCluff,andRandalDull21RoleofCaveolin-1intheRegulationofPulmonaryEndothelialPermeability303YuSun,RichardD.
Minshall,andGuochangHu22AssessmentofEndothelialPermeabilityandLeukocyteTransmigrationinHumanEndothelialCellMonolayers319AndreasLudwig,AnselmSommer,andStefanUhlig23PermeabilityofEndothelialBarrier:CellCultureandInVivoModels333AlexanderN.
Garcia,StephenM.
Vogel,YuliaA.
Komarova,andAsrarB.
Malik24Size-SelectiveandInVitroAssessmentofInnerBloodRetinaBarrierPermeability355MatthewCampbellandPeterHumphries25AssessmentofPermeabilityinBarrierTypeofEndotheliuminBrainUsingTracers:EvansBlue,SodiumFluorescein,andHorseradishPeroxidase369MehmetKayaandBulentAhishali26InVitroandInVivoMethodsforAssessingFcRn-MediatedReverseTranscytosisAcrosstheBlood–BrainBarrier383NadiaCaram-Salas,EveBoileau,GrahamK.
Farrington,EllenGarber,EricBrunette,AbedelnasserAbulrob,andDanicaStanimirovic27EvaluationofVEGF-InducedVascularPermeabilityinMice403SaraM.
WeisixContents28InVivoMeasurementofGlioma-InducedVascularPermeability417JisookLee,AndrewBaird,andBrianP.
Eliceiri29InVivoOpticalImagingofIschemicBlood–BrainBarrierDisruption423AbedelnasserAbulrob,EricBrunette,JacquelineSlinn,EwaBaumann,andDanicaStanimirovicIndex441wwwwwwwwwwwwwwwxiContributorsCatherineA.
AbbottSchoolofBiologicalSciences,FlindersUniversity,Adelaide,SA,AustraliaAbedelnasserAbulrobInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaJ.
StevenAlexanderDepartmentofMolecularandCellularPhysiology,LouisianaStateUniversityHealthSciencesCenter,Shreveport,LA,USABulentAhishaliDepartmentsofPhysiologyandHistologyandEmbryology,IstanbulFacultyofMedicine,IstanbulUniversity,Istanbul,TurkeyBogiAndersenDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAAndrewBairdDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USABrentM.
BanyDepartmentofPhysiologyandObstetricsandGynecology,SouthernIllinoisUniversitySchoolofMedicine,Carbondale,IL,USAEwaBaumannCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAmbicaBhandariDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAEveBoileauInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaEricBrunetteCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMatthewCampbellUCDSchoolofBiomolecularBiomedicalSciences,ConwayInstitute,UniversityCollegeDublin,Dublin,IrelandNadiaCaram-SalasInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMarcelinoCereijidoDepartmentofPhysiology,BiophysicsandNeurosciences,CenterforResearch&AdvancedStudies(CINVESTAV),Av.
InstitutoPolitécnicoNacional2508,Col.
SanPedroZacatenco,Del.
GAMC.
P.
07360MéxicoC.
YanChengCenterforBiomedicalResearch,PopulationCouncil,NewYork,NY,USAMarkCluffDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USARubenG.
ContrerasCenterforResearch&AdvancedStudies(CINVESTAV),MexicoCity,MéxicoWillemijnE.
CorpeleijnDivisionofNeonatology,ErasmusMC–SophiaChildren'sHospital,Rotterdam,TheNetherlandsxiiContributorsLídiaCorralizaDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainRinoP.
DonatoWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaCarrieA.
DuckworthGastroenterologyResearchUnit,SchoolofClinicalSciences,UniversityofLiverpool,Liverpool,UKRandalDullDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USARuurdM.
vanElburgDepartmentofPediatrics,DivisionofNeonatology,VrijeUniversiteitMedicalCenter,Amsterdam,TheNetherlandsBrianP.
EliceiriDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USAAdaweyahEl-MerhibiaWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaGrahamK.
FarringtonInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaEmmaR.
FormosaWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaEllenGarberInstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAlexanderN.
GarciaDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAMartaGarcia-RamírezDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainHelmutGattDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaJulieGavardInstitutCochin,UniversitéParisDescartes,UMR-CNRS8104,Paris,FranceKristinaGiantsosDepartmentofAnesthesiology,UniversityofUtahSchoolofMedicine,SaltLakeCity,UT,USAWilliamGordonDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAJohannesB.
vanGoudoeverDivisionofNeonatology,ErasmusMC–SophiaChildren'sHospital,Rotterdam,TheNetherlandsAlexanderGowCenterforMolecularMedicineandGenetics,WayneStateUniversity,Detroit,MI,USAArmelleLeGuelteInstitutCochin,UniversitéParisDescartes,UMR-CNRS8104,Paris,FranceBatjargalGundsambuuAWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaTsambikaHahnBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyxiiiContributorsG.
ScotHamiltonDepartmentofPhysiologyandPharmacology,UniversityofWesternOntario,London,ON,CanadaDanielHeklDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaMichaelHeisigGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,GermanyCristinaHernándezDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelona,Barcelona,SpainGuochangHuDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAPeterHumphriesUCDSchoolofBiomolecularBiomedicalSciences,ConwayInstitute,UniversityCollegeDublin,Dublin,IrelandArupKumarIndraDepartmentofPharmaceuticalSciences,CollegeofPharmacy,andEnvironmentalHealthSciencesCenter,OregonStateUniversity,Corvallis,OR,USAMehmetKayaDepartmentsofPhysiology&HistologyandEmbryology,IstanbulFacultyofMedicine,IstanbulUniversity,Istanbul,TurkeyIdoP.
KemaDepartmentofLaboratoryMedicine,DivisionofClinicalChemistryandHematology,UniversityMedicalCenterGroningen,Groningen,TheNetherlandsUllaG.
KnausConwayInstitute,UniversityCollegeDublin,Dublin,IrelandYuliaA.
KomarovaDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAIsabelLarreCenterforResearch&AdvancedStudies(CINVESTAV),MexicoCity,MéxicoJudithLechnerDivisionofPhysiology,DepartmentofPhysiologyandMedicalPhysics,DivisionofPhysiology,InnsbruckMedicalUniversity,Innsbruck,AustriaJisookLeeDepartmentofSurgery,UniversityofCaliforniaSanDiego,SanDiego,CA,USAMarkLeidDepartmentofPharmaceuticalSciences,CollegeofPharmacy,andEnvironmentalHealthSciencesCenter,OregonStateUniversity,Corvallis,OR,USAAndreasLudwigInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyBrigitteLeMagueresse-BattistoniInsermU870,Oullins,Oullins,France;2INRA,UMR1235,Oullins,France;3INSA-Lyon,RMND,Villeurbanne,FranceKaiYanMakaAWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaAsrarB.
MalikDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USASéverineMazaud-GuittotInsermU870,Oullins,France,2INRA,UMR1235,Oullins,France;3INSA-Lyon,RMND,Villeurbanne,FranceAnaMeleroBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyxivContributorsRichardD.
MinshallDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAElizabethMonaghan-BensonDepartmentofCellandDevelopmentalBiologyandLinebergerCancerCenter,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC,USADoloresD.
MrukCenterforBiomedicalResearch,PopulationCouncil,NewYork,NY,USAArneNaegelGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,GermanyBarryC.
PowellWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaBarryPressMaRSCentre,OntarioInstituteforCancerResearch,Toronto,ON,CanadaPeterRubenwolfDepartmentofPaediatricUrology,RegensburgUniversityHospital,KlinikSt.
HedwigRegensburg,GermanyMichaelL.
SalmansDepartmentsofMedicineandBiologicalChemistry,UniversityofCalifornia,Irvine,CA,USAUlrichF.
SchaeferBiopharmaceuticsandPharmaceuticalTechnology,SaarlandUniversity,Saarbrucken,GermanyMarcSchneiderPharmaceuticalNanotechnology,SaarlandUniversity,Saarbrucken,GermanyThomasSeppiDepartmentofTherapeuticRadiologyandOncology,InnsbruckMedicalUniversity,Innsbruck,AustriaRafaelSimóDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainJacquelineSlinnCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaAnselmSommerInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyJenniferSouthgateJackBirchUnitofMolecularCarcinogenesis,DepartmentofBiology,UniversityofYork,York,UKDanicaStanimirovicCerebrovascularResearchGroup,InstituteforBiologicalSciences,NationalResearchCouncilofCanada,Ottawa,ON,CanadaMonikaStrengertConwayInstitute,UniversityCollegeDublin,Dublin,IrelandYuSunDepartmentsofAnesthesiologyandPharmacologyandCenterforLungandVascularBiology,UniversityofIllinoisatChicago,Chicago,IL,USAStefanUhligInstituteofPharmacologyandToxicology,FacultyofMedicine,RWTHAachenUniversity,Aachen,GermanyMartaVillarroelDiabetesandMetabolismResearchUnit,InstitutdeRecercaHospitalUniversitariValld'Hebron,UniversitatAutònomadeBarcelonaandCIBERdeDiabetesyEnfermedadesMetabólicasAsociadas(CIBERDEM),InstitutodeSaludCarlosIII(ISCIII),Barcelona,SpainxvContributorsMarkusVoelpInnovativeTechnologie-Voelp(IT-V),Innsbruck,AustriaStephenM.
VogelDepartmentofPharmacology,UniversityofIllinois,Chicago,IL,USAXianWangWomen'sandChildren'sHealthResearchInstitute,NorthAdelaide,SA,AustraliaYupingWangDepartmentofObstetricsandGynecology,LouisianaStateUniversityHealthSciencesCenter,Shreveport,LA,USAAlastairJ.
WatsonGastroenterologyResearchUnit,SchoolofClinicalSciences,UniversityofLiverpool,Liverpool,UKSaraM.
WeisMooresUCSDCancerCenter,UniversityofCalifornia,SanDiego,LaJolla,CA,USAErikaS.
WittchenDepartmentofCellandDevelopmentalBiologyandLinebergerCancerCenter,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC,USAGabrielWittumGoethe-CenterforScientificComputing,Goethe-University,FrankfurtamMain,Germanywwwwwwwwwwwww
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