Ethylbenzeneddd13.com

T100775E1998Sigma-AldrichCo.
Bulletin775EThisbulletinoutlinestheUnitedStatesEnvironmentalProtec-tionAgency'sproceduresforanalyzing113organicprioritypollutantsinwastewater.
ThechromatographiccolumnorcolumnpackinglistedbytheEPA,anditsSupelcoequivalent,aredescribedforeachgaschromatographic-massspectro-metric,gaschromatographic,orhighperformanceliquidchromatographicmethod.
Confirmationalcolumnsandtrapsforconcentratinganalytesfromsamplesaredescribedformethodsinwhichtheyarespecified.
Theanalysesareillus-tratedwithchromatograms.
KeyWordsqwastewaterqprioritypollutantsqvolatilepollutantsqacidicpollutantsqbase/neutralpollutantsqpesticidesqPCBsTheUnitedStatesEnvironmentalProtectionAgency(USEPA)hasamendeditsprioritypollutantprotocol(1)byrevisingthegaschromatographic-massspectrometricmethods(GC-MS)andde-velopingalternativegasorhighperformanceliquidchromato-graphicmethods(GCorHPLC)fororganicwaterpollutantanaly-ses.
Thesealternativemethodsofanalysisenableeachregulatedindustrytochoosetheapproachthatsuitsitsindividualneedsorcapabilities.
TheEPAconsidersthesemethods,describedintheFederalRegister(2),tobetheonlyvalidatedanalyticalapproachtomonitoringprioritypollutantsinwastewater.
Industriesarere-quiredtousethemethodsbothinevaluatingtheirwastewaterandinapplyingforNationalPollutionDischargeEliminationSystem(NPDES)permits(3).
DeviationsfromthesemethodsrequireEPAapprovalinadvance.
Generalinformationabouttheproceduresfollows,precedingillustrationsandbriefoutlinesoftheanalyses.
GC-MSAnalysesforOrganicPollutantsFortherevisedGC-MSanalyses,theorganicpollutantsareclassi-fiedasvolatilesorasnonvolatileacids(phenols),base-neutrals,andpesticidesandPCBs,astheywereinthe1977protocol(1).
Thechromatographiccolumnsdescribedintheoriginalprotocol,however,havebeenreplacedwithcolumnsdevelopedthroughadvancedcolumntechnology.
Thenewerpackingsaremorethoroughlydeactivatedthanthepackingstheyreplace,anduseofthecolumnslistedintherevisedmethodsimprovesthedetectionofprioritypollutants.
TheGC-MSanalysesfortheorganiccom-poundsareincorporatedintotwomethods,Method624forvolatilesandMethod625fornonvolatiles.
GCorLCOrganicPollutantAnalysesTheGCorHPLCmethods(Methods601-613)simplifychromato-graphicanalysesbyseparating113organicpollutantsinto13classesanddescribingananalysisforeachclass.
EachGCorHPLCUSEPAProceduresforWastewaterAnalysesbyPackedColumnGCandHPLCanalysisspecifiestheuseofachromatographiccolumnthatwillresolveallofthepollutantsinoneclassandadetectionsystemthatisspecificfortheiridentification(2).
Certainmethodsalsosuggestusingaconfirmationalcolumntosubstantiatetheidentityofthepollutants.
SupelcoGCpackingswereusedbytheEPAtoevaluatethesemethodsforprioritypollutantanalyses.
SupelcopackingsarelistedbytheEPAinmanyofthemethods,andformostoftheothermethodsSupelcopackingsaregenericallyequivalentalternativestothelistedpackings.
Supelcopackingsandcolumnswereusedtoobtainallofthechromatogramsinthisbulletin.
ConcentrationofOrganicPollutantsOrganicpollutantsnormallyarepresentatppborlowerlevelsinwastewatersamples,andtheymustbeconcentratedpriortochromatographicanalysis.
Thevolatileorganicsarepurgedfromthewatersampleandaretrappedonsolidadsorbents(GC-MSMethod624orGCMethod601,602,or603).
Therecommendedpurge-trapmethodhasbeendescribedbyBellarandLichtenberg(4).
Aftertheorganicsareadsorbed,theyarerapidlyheatdesorbedfromthetrapontothechromatographiccolumn.
TheEPArecom-mendsadifferentadsorbenttrapforeachofthefourmethodsofanalysisforvolatilepollutants.
Eachtrapprovidesthebesttrappingefficiencyforthevolatilecompoundslistedinthecorrespondingmethod.
Thenonvolatileorganicsareconcentratedbysolventextractionofthewatersamplefollowedbyconcentrationoftheextractto1mLinaKuderna-Danishconcentrator.
Subsequently,theextractisdriedonasodiumsulfatecolumn.
Whenwatersamplesareextremelydirty,specificcleanupproceduresareusedwitheachextractionmethod.
DetailsoftheextractiontechniquesandcleanupproceduresaregivenintheFederalRegister(2).
OutlinesofEPAMethodsOutlinesoftheGC-MSmethodsfororganicpollutantanalysesarepresentedfirst,followedbyoutlinesoftheGCandHPLCmethods.
Eachmethodoutlineliststheclassoforganiccompounds,theconcentrationtechnique,thecolumn,theconfirmationalcolumn(ifany),thedetector,andanyqualificationsorpotentialproblemsthathavebeenindicatedbytheEPA.
FormethodsinwhichthelistedpackingisnotaSupelcopacking,theSupelcoequivalentisgiveninparentheses.
Wehaveusedtwo-meterglasscolumnsforthosemethodsthatcallforanominalsix-footglasscolumn.
Two-meterglasscolumnsgivemoreconsistentresults,throughtightercontrolofthecolumnlengthandinternaldiameter,andthusofthevolumeofchromatographicpacking.
ThesemethodsarediscussedindetailintheFederalRegister(2).
Thechromatographicpackingsandcolumnsusedintheseanaly-sesarelistedfollowingthemethodoutlines.
SUPELCOBulletin7752GC-MSMethod624–VolatilePollutantsFigureA.
VolatilesbyEPAMethod624Column:60/80CarbopackB/1%SP-1000,8'x1/8"ODSSCat.
No.
:11815(15gpacking)Oven:45°C(3min)to220°Cat8°C/min,hold15minCarrier:helium,40mL/minDet.
:FID,250°CInj.
:1Ldodecanecontaining200-500ng/Leachanalyte,200°CSampleConcentrationPurgeandtrap,usinga30.
5cmx0.
105"IDx0.
125"ODSStrapcontaining15cmTenax,8cmSilicaGel15,and1cm3%SP-2100.
GCColumn6'x1/8"ODSScontaining60/80CarbopackB/1%SP-1000.
FigureAshowstheanalysisonan8'columnalsousedinMethod601.
A2"x1/8"ODSSprecolumncontaining3%SP-1000on60/80ChromosorbWAWisrecommended.
ConfirmationalColumn8'x1/8"ODSScontaining60/80CarbopackC/0.
2%CARBOWAX1500.
A2"x1/8"ODSSprecolumncontaining3%CARBOWAX1500on60/80ChromosorbWAWisrecommended.
DetectorMassspectrometer.
ForFigureAanFIDwasusedtosimulatethemassspectrometeranalysis.
QualificationsAcroleinandacrylonitrilearevolatilecompounds,butpurgingefficienciesforthemarelowanderratic.
Forthesecompounds,theEPArecommendsdirectaqueousinjectionorthemodifiedpurge-trapproceduredescribedinEPAMethod603.
GC-MSMethod625–NonvolatilePollutantsI.
BaseNeutralsFigureB.
Base-NeutralsbyEPAMethod625Column:3%SP-2250on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11756(20gpacking)Oven:50°C(4min)to270°Cat8°C/minCarrier:nitrogen,30mL/minDet.
:FIDInj.
:1Lmethylenechloridecontaining0.
125mg/mLor0.
063mg/mL(*)eachanalyte713-0704123456789101112131415161718192023242526273230312928444036322824201612840Min12345678910111213141516171819202324252627323031292821223433713-10374036322824201612840Min484452SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx2mmIDglasscontaining3%SP-2250on100/120SUPELCOPORT.
ConfirmationalColumnNonelisted.
DetectorMassspectrometer.
ForFigureBanFIDwasusedtosimulatethemassspectrometeranalysis.
QualificationsAnalystsmustbeabletochromatograph100ngofbenzidinetodemonstratetheinertnessoftheGC-MSsystembeforetheyusethisprocedureonwastewatersamples.
TheEPAhassetthedetec-tionlimitformostofthebase-neutralsat20ng/L.
For6-and7-ringpolycyclicaromatics,however,thelimitis50ng/L.
21221.
Chloromethane2.
Methanol3.
Bromomethane4.
Vinylchloride&Dichlorofluoromethane5.
Chloroethane6.
Methylenechloride7.
Trichlorofluoromethane8.
1,1-Dichloroethylene9.
Bromochloromethane(IS)10.
1,1-Dichloroethane11.
trans-1,2-Dichloroethylene12.
Chloroform13.
1,2-Dichloroethane14.
1,1,1-Trichloroethane15.
Carbontetrachloride16.
Bromodichloromethane17.
1,2-Dichloropropane18.
trans-1,3-Dichloropropene19.
Trichloroethylene20.
Benzene21.
cis,1,3-Dichloropropene,1,1,2-Trichloroethane,&Chlorodibromomethane22.
1-Chloro-2-bromopropane(IS)23.
2-Chloroethylvinylether24.
Bromoform25.
1,1,2,2-Tetrachloroethylene&1,1,2,2-Tetrachloroethane26.
1,4-Dichlorobutane(IS)27.
Toluene28.
Chlorobenzene29.
Ethylbenzene30.
1,3-Dichlorobenzene31.
1,2-Dichlorobenzene32.
1,4-Dichlorobenzene1.
1,3-Dichlorobenzene2.
1,4-Dichlorobenzene3.
bis(2-Chloroethyl)ether4.
Hexachloroethane&1,2-Dichlorobenzene5.
bis(Methyl-2-chloroethyl)ether6.
N-Nitroso-di-n-propylamine7.
Nitrobenzene&Hexachlorobutadiene8.
1,2,4-Trichlorobenzene&Isophorone9.
Naphthalene&bis(2-Chloroethoxy)methane10.
Hexachlorocyclopentadiene11.
2-Chloronaphthalene12.
Acenaphthylene13.
Acenaphthene14.
Dimethylphthalate15.
2,6-Dinitrotoluene16.
Fluorene&4-Chlorophenylphenylether17.
2,4-Dinitrotoluene18.
Diethylphthalate&N-Nitrosodiphenylamine19.
4-Bromophenylphenylether&Hexachlorobenzene20.
Phenanthrene&Anthracene21.
Di-n-butylphthalate22.
Fluoranthene*23.
Pyrene*24.
Benzidine25.
Benzylbutylphthalate26.
bis(2-Ethylhexyl)phthalate27.
Benz(a)anthracene*28.
Chrysene*29.
3,3'-Dichlorobenzidine30.
Di-n-octylphthalate31.
Benzo(b)fluoranthene*&Benzo(k)fluoranthene*32.
Benzo(a)pyrene*33.
Indeno(1,2,3-cd)pyrene*&Dibenz(a,h)anthracene*34.
Benzo(ghi)perylene3SUPELCOBulletin775II.
Acidics(Phenols)FigureC.
PhenolsbyEPAMethod625Column:1%SP-1240-DA*on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11832(20gpacking)Oven:70°C(2min)to200°Cat8°C/min,holdCarrier:helium,30mL/minDet.
:FIDInj.
:0.
5Lstandardphenolmixture(Cat.
No.
48810)04812162024121110987654321795-0194MinSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx2mmIDglasscontaining1%SP-1240-DA*on100/120SUPELCOPORT.
ConfirmationalColumnNonelisted.
DetectorMassspectrometer.
ForFigureCanFIDwasusedtosimulatethemassspectrometeranalysis.
QualificationsAnalystsmustchromatograph50ngofpentachlorophenoltoevaluatetheinertnessoftheirGC-MSsystembeforetheyusethismethodtoanalyzewastewatersamplesforphenols.
*DA–Deactivatedforacidiccompounds.
0481216202428121110987654321Min32795-0193SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
ThespecificprocedureisdescribedinEPAMethod608.
GCColumn2mx2mmIDglasscontaining3%SP-2250on100/120SUPELCOPORT.
ConfirmationalColumnNonelisted.
DetectorMassspectrometer(seeQualifications).
ForFigureDanFIDwasusedtosimulatethemassspectrometeranalysis.
QualificationsThesampleisanalyzedontheGCcolumnwithanelectroncapturedetector(ECD)asdescribedinGCMethod608.
AGC-MSanalysisisperformedonlyiftheGC/ECDanalysisindicatesthepresenceofpesticidesorPCBs.
Pesticideswithmultiplepeaks(PCBs,toxaphene,andchlordane)canbeseparatedfromtheotherpesticidesbysamplecleanupwithFlorisilcolumnchromatography(5).
Analystsmustbeabletochromatograph100ngofaldrinbeforetheyusetheGC-MSprocedureonwastewatersamples.
QuantificationofPriorityPollutantsbyGC-MSInternalstandardsmaybeusedinanyoftheGC-MSanalyses,iftheyareadequatelyresolvedfromtheprioritypollutantsinthesampleandfrommatrixinterferences.
TwogroupsofinitialstandardsrecommendedbytheEPAaredeuteratedandfluorinatedcom-poundsofvariousaromatics,phenols,amines,andnitroaromatics.
Amassspectrumofeither4-bromofluorobiphenylordecafluorotriphenylphosphinemustbecheckeddailytomeetperformancecriteriasetforthinMethod625.
Ifexternalstandardsareusedtocalibrate,individualstandardsofeachcompoundmustbeanalyzed.
Athree-pointcalibrationmustbeperformed,withonepointofthecalibrationcurvenearthelimitofdetection.
Thecalibrationmustbeverifieddailywithatleastoneofthestandardsolutionsusedintheinitialcalibration.
Ifsignificantvariation(±10%)occurs,anewcalibrationshouldbeconducted.
13InMethod625,capillarycolumnsmaybeusedasanalternativetopackedcolumnsiftheymeettheQCcriterialistedinSections8.
2,12,and13.
1(4).
Twoavailableprotocolsforcapillarymethodologyare2&3below:1.
MethodofOrganicChemicalAnalysisofMunicipalandIndustrialWastewaterUSEPAEMSL,Cincinnati,OH45268,EPA-600/4-83-057,July1982.
2.
TestingMethodsforEvaluatingSolidWasteUSEPAOfficeofSolidWasteandEmergencyResponse,Washington,D.
C.
,20460,SW-846,July,1982,2ndEdition.
3.
A.
D.
Sauter,etal.
,J.
HighResolutionChromatographyandChroma-tographyCommunications4:366(1981).
1.
Methanol2.
o-Chlorophenol,0.
5mg/mL3.
2-Nitrophenol,0.
5mg/mL4.
Phenol,0.
5mg/mL5.
2,4-Dimethylphenol,0.
5mg/mL6.
2,4-Dichlorophenol,0.
5mg/mL7.
2,4,6-Trichlorophenol,1.
5mg/mL8.
4-Chloro-m-cresol,2.
5mg/mL9.
2,4-Dinitrophenol,1.
5mg/mL10.
4,6-Dinitro-o-cresol,2.
5mg/mL11.
Pentachlorophenol,2.
5mg/mL12.
4-Nitrophenol,2.
5mg/mLIII.
PesticidesandPCBsFigureD.
PesticidesbyEPAMethod625Column:3%SP-2250on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11756(20gpacking)Oven:50°C(4min)to270°Cat8°C/minCarrier:nitrogen,30mL/minDet.
:FIDInj.
:0.
1L,1ng/Leachanalyte1.
α-BHC2.
γ-BHC3.
β-BHC4.
Heptachlor5.
δ-BHC6.
Aldrin7.
Heptachlorepoxide8.
EndosulfanI9.
Dieldrin&p,p'-DDE10.
Endrin11.
EndosulfanII&p,p'-DDD12.
p,p'-DDT13.
Endosulfansulfate&EndrinaldehydeSUPELCOBulletin7754GCMethod601–PurgeableHalocarbonsSampleConcentrationPurgeandtrap,usinga30.
5cmx0.
105"IDx0.
125"ODSStrapcontaining7.
7cmactivatedcharcoal,7.
7cmSilicaGel15,7.
7cmTenax,and1cm3%SP-2100.
GCColumn8'x1/8"ODSScontaining60/80CarbopackB/1%SP-1000.
ConfirmationalColumn8'x1/8"ODSScontainingn-octaneon80/100PorasilC.
DetectorHallelectroconductivity.
QualificationsElectroncapturedetectorsarenotrecommendedforthisanalysisbecausetheyhavelimitedsensitivityformono-anddi-substitutedhalocarbons.
GCMethod602–PurgeableAromaticsSampleConcentrationPurgeandtrap,usinga30.
5cmx0.
105"IDx0.
125"ODSStrapcontaining1cm3%SP-2100and23cmTenax.
GCColumn6'x1/8"ODSScontaining5%SP-1200/1.
75%Bentone34on100/120SUPELCOPORT.
ConfirmationalColumn8'x1/8"ODSScontaining5%1,2,3-tris(2-cyano-ethoxy)propaneon60/80ChromosorbWAW.
DetectorPhotoionization(10.
2evlamp).
ForFigureFanFIDwasusedtosimulatethephotoionizationdetector.
QualificationsFIDdetectioncanbeusedifinterferingorganicscanberesolvedfromthepurgeablearomatics.
GCMethod603–AcroleinandAcrylonitrileSampleConcentrationPurgeandtrap,usinga30.
5cmx0.
105"IDx0.
125"ODSStrapcontaining1cm3%SP-2100and23cmTenax.
GCColumn6'x1/8"ODSScontainingDurapak/Carbowax400on100/120PorasilC.
*ConfirmationalColumn6'x1/8"ODSScontaining80/100Chromosorb101.
DetectorFID.
*NOTE:100/120PorasilCisnolongeravailablefromthemanufacturer.
FigureE.
PurgeableHalocarbonsbyEPAMethod601Column:60/80CarbopackB/1%SP-1000,8'x1/8"ODSSCat.
No.
:11815(15gpacking)Oven:45°C(3min)to220°Cat8°C/min,hold15minCarrier:nitrogen,40mL/minDet.
:Hall,85°CInj.
:1Ldodecanecontaining200-500ng/Leachanalyte,200°C123456789101112131415161718192023242122795-0179201612840Min1234567795-0180201612840MinFigureG.
AcroleinandAcrylonitrilebyEPAMethod603Column:80/100Chromosorb101,6'x1/8"ODSSCat.
No.
:20214(50gpacking)Oven:100°C(5min)to140°Cat10°C/min,holdCarrier:helium,45mL/minDet.
:FIDInj.
:2LwaterAcrolein,3mg/mLAcrylonitrile,1mg/mL795-018112840Min1.
Chloromethane2.
Bromomethane3.
Vinylchloride&Dichlorofluoromethane4.
Chloroethane5.
Methylenechloride6.
Trichlorofluoromethane7.
1,1-Dichloroethylene8.
Bromochloromethane(IS)9.
1,1-Dichloroethane10.
trans-1,2-Dichloroethylene11.
Chloroform12.
1,2-Dichloroethane13.
1,1,1-Trichloroethane14.
Carbontetrachloride15.
Bromodichloromethane16.
1,2-Dichloropropane&trans-1,3-Dichloropropane17.
Trichloroethylene18.
cis-1,3-Dichloropropene,1,1,2-Trichloroethane,&Chlorodibromomethane19.
1-Chloro-2-bromopropane(IS)20.
2-Chloroethylvinylether21.
Bromoform22.
1,1,2,2-Tetrachloroethylene&1,1,2,2-Tetrachloroethane23.
1,4-Dichlorobutane(IS)24.
ChlorobenzeneFigureF.
PurgeableAromaticsbyEPAMethod602Column:5%SP-1200/1.
75%Bentone34on100/120SUPELCOPORT,6'x1/8"SSCat.
No.
:12134(20gpacking)Oven:50°C(2min)to90°Cat6°C/min,holdCarrier:helium,36mL/minDet.
:FIDInj.
:1Lmethanolcontaining0.
2mg/mLeachanalyte1.
Benzene2.
Toluene3.
Ethylbenzene4.
Chlorobenzene5.
1,4-Dichlorobenzene6.
1,3-Dichlorobenzene7.
1,2-Dichlorobenzene5SUPELCOBulletin775GCMethod604–PhenolsFigureH.
PhenolsbyEPAMethod604Column:1%SP-1240-DA*on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11832(20gpacking)Oven:70°C(2min)to200°Cat8°C/min,holdCarrier:helium,30mL/minDet.
:FID,250°CInj.
:0.
5Lstandardmix(Cat.
No.
48859)234567891101112713-1032201612840Min24SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn(FreePhenols)2mx2mmIDglasscontaining1%SP-1240-DA*on100/120SUPELCOPORT.
TheanalysisissimilartotheGC-MSanalysisinFigureC.
ConfirmationalColumnNonelisted.
DetectorFID.
QualificationsWhensamplecleanupisrequired,theextractedphenolsarederivatizedwithpentafluorobenzylbromide(PFB)andtheextractiscleanedbysilicagelcolumnchromatography.
PFBphenolsareseparatedonacolumndifferentfromthatusedtoseparatefreephenols,andaredetectedbyelectroncapture.
GCColumn(DerivatizedPhenols)1.
8mx2mmIDglasscontaining5%OV-17on80/100ChromosorbWAW-DMCS.
(SupelcoEquivalent:5%SP-2250on80/100SUPELCOPORT.
)DetectorElectroncapturedetector.
*DA–Deactivatedforacidiccompounds.
SampleConcentrationSolventextractionwithchloroformfollowedbyconcentrationto1mL.
HPLCColumn25cmx4.
6mmIDSScontainingLiChrosorbRP-2.
(SupelcoColumn:25cmx4.
6mmIDSScontainingSUPELCOSILLC-1).
ConfirmationalColumnNonelisted.
DetectorElectrochemical(0.
8volts).
ForFigureI,UVdetectionat254nmwasused(seeQualifications).
QualificationsAUVdetectormaybeusediftherearenomatrixinterferences.
TheEPAproceduresuggestsusinga25cmx4.
6mmIDcolumn,butamorethanadequateseparationisreadilyachievedwitha5cmx4.
6mmIDora15cmx4.
6mmIDSUPELCOSILLC-1column.
797-0651420Min1.
Methanol2.
o-Chlorophenol(0.
25g)3.
2-Nitrophenol(0.
25g)4.
Phenol(0.
25g)5.
2,4-Dimethylphenol(0.
25g)6.
2,4-Dichlorophenol(0.
25g)7.
2,4,6-Trichlorophenol(0.
75g)8.
4-Chloro-m-cresol(1.
25g)9.
2,4-Dinitrophenol(0.
75g)10.
4,6-Dinitro-p-cresol(1.
25g)11.
Pentachlorophenol(1.
25g)12.
4-Nitrophenol(1.
25g)HPLCMethod605–Benzidineand3,3'-DichlorobenzidineFigureI.
BenzidinesbyEPAMethod605Column:SUPELCOSILLC-1,5cmx4.
6mm,5mparticlesCat.
No.
:58237MobilePhase:0.
1Macetatebuffer:acetonitrile,60:40FlowRate:0.
8mL/minDet.
:UV,254nmInj.
:10Lmobilephasecontaining0.
5geachanalyteSolventfront3,3'-DichlorobenzidineBenzidineSUPELCOBulletin7756GCMethod606–PhthalateEstersSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx4mmIDglasscontaining1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT.
Twotemperatures,180°Cand220°C,areused.
ConfirmationalColumn2mx4mmIDglasscontaining3%OV-1on100/120SUPELCOPORT.
(SupelcoEquivalent:3%SP-2100on100/120SUPELCOPORT.
)DetectorElectroncapturedetector.
QualificationsIftheextractmustbecleaned,theEPArecommendsFlorisilorsilicagelcolumnchromatography.
211795-0185241680Min8016MinGCMethod607–NitrosaminesSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx4mmIDglasscontaining10%CARBOWAX20M/2%KOHon80/100ChromosorbWAW.
Twotemperatures,110°Cand220°C,areused.
ConfirmationalColumn2mx4mmIDglasscontaining10%SP-2250on100/120SUPELCOPORT.
DetectorNitrogen-phosphorousdetector.
ForFigureKanFIDwasusedinplaceofanitrogen-phosphorousdetector.
QualificationsN-NitrosodiphenylaminedecomposesintheGCinjectionportandisdetectedasdiphenylamine.
Therefore,diphenylamineinthesamplemustberemovedbyFlorisilcolumnchromatographyoritwillinterferewiththeanalysis.
1234567891011121314795-01871612840Min15GCMethod608–OrganochlorinePesticidesandPCBsSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx4mmIDglasscontaining1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT.
ConfirmationalColumn2mx2mmIDglasscontaining3%OV-1on100/120SUPELCOPORT.
(SupelcoEquivalent:3%SP-2100on100/120SUPELCOPORT.
)DetectorElectroncapturedetector.
QualificationsTheextractmustbecleanedbyFlorisilcolumnchromatographytoeliminatepossiblephthalateinterference(5).
1.
Dimethylphthalate,1.
0ppm2.
Diethylphthalate,0.
2ppm3.
Di-n-butylphthalate,1.
0ppm231231840Min795-0183201612840MinFigureK.
NitrosaminesbyEPAMethod607Column:10%CARBOWAX20M/2%KOHon80/100ChromosorbWAW,2mx4mmIDglassCat.
No.
:11739(20gpacking)Carrier:helium,40mL/minDet.
:FIDInj.
:2Lmethanol1.
N-Nitrosodimethylamine,0.
2mg/mL2.
N-Nitroso-di-n-propylamine,0.
2mg/mL1.
N-Nitrosodiphenylamine(asDiphenylamine),0.
4mg/mLFigureL.
PesticidesbyEPAMethod608Column:1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT,2mx4mmIDglassCat.
No.
:11947(20gpacking)Oven:200°CCarrier:argon:methane,95:5,6.
0mL/minDet.
:ECNi63Inj.
:0.
5Lcontaining0.
02mg/mLeachanalyte1.
α-BHC2.
γ-BHC3.
β-BHC4.
Heptachlor5.
δ-BHC6.
Aldrin7.
Heptachlorepoxide8.
EndosulfanI9.
p,p'-DDE10.
Dieldrin11.
Endrin12.
p,p'-DDD&EndosulfanII13.
p,p'-DDT14.
Endrinaldehyde15.
Endosulfansulfate180°C220°C110°CFigureJ.
PhthalatesbyEPAMethod606Column:1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT,2mx4mmIDglassCat.
No.
:11947(20gpacking)Carrier:nitrogen,60mL/minDet.
:ECD,250°CInj.
:1L1.
Butylbenzylphthalate,1.
0ppm2.
bis(2-Ethylhexyl)phthalate,0.
4ppm3.
Di-n-octylphthalate,1.
0ppm220°C7SUPELCOBulletin7752121795-0188840Min840MinSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn4'x4mIDglasscontaining1.
95%QF-1/1.
5%OV-17on80/100Gas-ChromQ.
(SupelcoEquivalent:2mx4mmIDglasscontain-ing1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT.
)Twotemperatures,85°Cand145°C,areused.
ConfirmationalColumn10'x4mmIDglasscontaining3%OV-101on80/100Gas-ChromQ.
(SupelcoEquivalent:3%SP-2100on100/120SUPELCOPORT.
)DetectorFIDforisophoroneandnitrobenzene;electroncapturedetectorfornitrotoluenes.
QualificationsTheSupelcoequivalentcolumnistwometerslong,ratherthatfourfeetlong,topermititsapplicationtoMethods606,608,and613.
25cmx4.
6mmcolumn,5mparticles5cmx4.
6mmcolumn,3mparticles12345678910111213241680Min1514164032123456789101112131514163210Min4797-0652,796-0386241680Min32795-0189404812345678910111213GCMethod609–NitroaromaticsandIsophoroneFigureM.
NitroaromaticsandIsophoronebyEPAMethod609Column:1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT,2mx4mmIDglassCat.
No.
:11947(20gpacking)Carrier:nitrogen,44mL/minDet.
:FID(nitrobenzene,isophorone)orECD(dinitrotoluenes)Inj.
:2L,0.
2mg/mLeachanalyte(nitrobenzene,isophorone)or0.
5L,2ng/Leachanalyte(dinitrotoluenes)GCorLCMethod610–PolynuclearAromaticHydrocarbons(PAHs)FigureN.
PAHsbyHPLCbyEPAMethod610Columns:SUPELCOSILLC-PAHCat.
No.
:58229(top)or59133MobilePhase:water(A):acetonitrile(B)gradienttop–40%B(5min)to100%Bover25min,holdbottom–60%B(0.
3min)to100%Bover2.
7min,hold1minFlowRate:1mL/min(top)or3.
0mL/minDet.
:UV,254nmInj.
:2Lmethanol/methylenechloridecontaining0.
4geachanalyte1.
Naphthalene2.
Acenaphthylene3.
Acenaphthene4.
Fluorene5.
Phenanthrene6.
Anthracene7.
Fluoranthene8.
Pyrene9.
Benz(a)anthracene10.
Chrysene11.
Benzo(b)fluoranthene12.
Benzo(k)fluoranthene13.
Benzo(a)pyrene14.
Dibenz(a,h)anthracene15.
Benzo(ghi)perylene16.
Indeno(1,2,3-cd)pyreneFigureO.
PAHsbyGCbyEPAMethod610Column:3%SP-2250on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11744(20gpacking)Oven:100°C(4min)to280°Cat8°C/min,holdCarrier:nitrogen,40mL/minDet.
:FIDInj.
:1Lmethanol/methylenechloridecontaining0.
2geachanalyte1.
Naphthalene2.
Acenaphthylene3.
Acenaphthene4.
Fluorene5.
Phenanthrene&Anthracene6.
Fluoranthene7.
Pyrene8.
Benz(a)anthracene9.
Chrysene10.
Benzo(b)fluoranthene&Benzo(k)fluoranthene11.
Benzo(a)pyrene12.
Dibenz(a,h)anthracene&Indeno(1,2,3-cd)pyrene13.
Benzo(ghi)perylene85°C1.
Nitrobenzene2.
Isophorone1.
2,6-Dinitrotoluene2.
2,4-Dinitrotoluene145°CSUPELCOBulletin7758Method610(contd.
)SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
HPLCColumn25cmx4.
6mmIDSScontainingHC-ODSSil-Xreversedphase.
(SupelcoEquivalent:25cmx4.
6mmIDSScontainingSUPELCOSILLC-PAH.
)ConfirmationalColumnNonelisted.
DetectorFluorescence.
GCColumn2mx2mmIDglasscontaining3%OV-17on100/120ChromosorbWAW-DMCS.
(SupelcoEquivalent:3%SP-2250on100/120SUPELCOPORT.
)ConfirmationalColumnNonelisted.
DetectorFID.
QualificationsTheHPLCprocedureisrecommendedforcompleteresolutionofthePAHs;theGCprocedurecannotadequatelyresolvefourpairsofthesecompounds.
TheEPAmethodsuggestsusinga25cmx4.
6mmIDHPLCcolumncontaining5mpackingparticlesforthisanalysis,butshorterSUPELCOSILLC-PAHcolumns(15cmx4.
6mmID,5mparticlesor5cmx4.
6mmID,3mparticles)cangreatlyreducetheanalysistime,asFigureNshows.
GCMethod611–HaloethersFigureP.
HaloethersbyEPAMethod611Column:3%SP-1000on100/120SUPELCOPORT,2mx2mmIDglassCat.
No.
:11746(20gpacking)Oven:60°C(2min)to230°Cat8°C/min,holdCarrier:helium,40mL/minDet.
:FIDInj.
:1Lmethanolcontaining0.
2mg/mLeachanalyte12345795-01901612840Min20SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mLGCColumn2mx2mmIDglasscontaining3%SP-1000on100/120SUPELCOPORT.
ConfirmationalColumn2mx2mmIDglasscontaining60/80Tenax.
DetectorHallelectroconductivity.
AnFIDwasusedtoobtainFigureP.
QualificationsThesampleshouldbecleanedonFlorisilifinterferencesaresuspectedtoexistinthesample.
GCMethod612–ChlorinatedHydrocarbonsFigureQ.
ChlorinatedHydrocarbonsbyEPAMethod612Column:1.
5%OV-1/1.
5%OV-225on80/100SUPELCOPORT,2mx2mmIDglassCat.
No.
:custom-preparedCarrier:argon:methane,95:5,30mL/minDet.
:ECDInj.
:2Lhexanecontaining0.
1-10ng/Leachanalyte123456789795-019112840Min840MinSampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
GCColumn2mx2mmIDglasscontaining1.
5%OV-1/2.
4%OV-225on80/100SUPELCOPORT(replaces1.
5%OV-1/1.
5%OV-225packingusedforFigureQ.
)Twotemperatures,75°Cand160°C,areused.
ConfirmationalColumnNonelisted.
DetectorElectroncapturedetector.
QualificationsSamplecleanup,ifrequired,isconductedonaFlorisilcolumn.
1.
bis(2-Chloroisopropyl)ether2.
4-Chlorophenylphenylether3.
bis(2-Chloroethoxy)methane4.
bis(2-Chloroethyl)ether5.
4-Bromophenylphenylether1.
1,3-Dichlorobenzene2.
1,4-Dichlorobenzene3.
Hexachloroethane4.
1,2-Dichlorobenzene5.
Hexachlorobutadiene6.
1,2,4-Trichlorocyclopentadiene7.
Hexachlorocyclopentadiene8.
2-Chloronaphthalene9.
Hexachlorobenzene75°C160°C9SUPELCOBulletin775GCMethod613–2,3,7,8-Tetrachlorobenzo-p-dioxin(TCDD)FigureR.
2,3,7,8-TCDDandOtherTCDDIsomersbyEPAMethod613Column:SP-2331capillarycolumn,60mx0.
32mmID,0.
2mfilmCat.
No.
:24105Oven:200°C(1min)to250°Cat3°C/minCarrier:helium,30cm/secDet.
:ECDInj.
:0.
2Ln-dodecanecontaining0.
2ngeachisomer,splitlessinjection,250°C1,4,7,8-TCDD2,3,7,8-TCDD1,2,3,7-TCDDand1,2,3,8-TCDD1,2,3,4-TCDD2,3,7,8-TCDD1,4,7,8-TCDD1,2,7,8-TCDD713-106512840Min16Onceagraphofthestandardconcentrationsvs.
responseisestablished,itischeckeddailytoensurethatnosignificantvariationhasoccurredinthestandardcurve.
Manyofthemethodsrecommendthatsurrogatestandardsbeincludedinallstandards,samples,andblanksforevaluationoftherecoveryandprecisionofthesampleworkupandanalysis.
Ifdoubtexistsabouttheidentityofapeakinthechromatographicanalysis,massspectrom-etryshouldbeusedforconfirmation.
Internalstandards,surrogates,andcalibrationstandardsrequiredintheGC-MSortheGCorLCmethodsarelistedinthechemicalstandardssectionoftheSupelcocatalog.
UseaCapillaryColumntoAnalyzeDifficultorComplexSamplesInUSEPAMethod625,capillarycolumnsmaybeusedasanalternativetopackedcolumnsiftheymeettheQCcriterialistedinSections8.
2,12,and13.
1(seepage3).
0.
25mmID,0.
32mmID,and0.
53mmIDSPB-5capillarycolumnsprovidesimilarresolu-tionofacidandbase/neutralpollutantsforMethod625(FigureS).
Inaddition,youcanuse0.
53mmIDcolumnsinpackedcolumnchromatographs.
InaGC-MSsystemwithahighcapacitypumporjetseparator,a0.
53mmIDcolumncombineslargesamplecapacity(upto2000ng/component)withrapidanalysisandgoodresolution.
SampleConcentrationSolventextractionwithmethylenechloridefollowedbyconcen-trationto1mL.
Thesampleiscleanedonasilicagelcolumn,thenonanaluminacolumn.
GCColumn60mx0.
32mmIDcapillarycolumncontainingSP-2331.
DetectorMassspectrometer.
QualificationsAseparatemethodwasestablishedforTCDDanalysisbecauseoftheextremetoxicityofthiscompound.
Thismethodshouldbereviewedthoroughlybeforeitisused.
A60mx0.
32mmIDcapillarycolumncontainingSP-2331resolves2,3,7,8-TCDDfromotherTCDDisomers.
Foradditionalinforma-tiononthisanalysis,requestApplicationNote113.
QuantificationofPriorityPollutantsbyGCorLCTheEPAalsohasrecommendedcalibrationproceduresfortheGCorLCanalysesofprioritypollutants.
Priortoanycalibration,thechromatographermustdemonstratethatthesystemisopera-tionalbyinjectingaknownmixtureofthecomponentstobeanalyzed.
Ablankcontainingallreagentsusediscarriedthroughtheextrac-tionandcleanupproceduresandisanalyzedtoensuretheabsenceofinterferences.
Concentrationsofcalibrationstandardsarecho-sentobrackettheexpectedconcentrationofthecompoundinthesample.
Thesestandardsestablishthesensitivitylimitofthedetectorandthelinearrangeoftheanalyticalsystemforeachcomponent.
N-NitrosodimethylaminePhenol2-Chlorophenolbis(2-Chloroethyl)ether1,3-Dichlorobenzene1.
1,4-Dichlorobenzene-d41,4-Dichlorobenzene1,2-Dichlorobenzenebis(2-Chloroisopropyl)etherHexachloroethaneN-Nitroso-di-n-propylamineNitrobenzeneIsophorone2-Nitrophenol2,4-Dimethylphenolbis(2-Chloroethoxy)methane2,4-Dichlorophenol1,2,4-Trichlorobenzene2.
Naphthalene-d8NaphthaleneHexachlorobutadieneFigureS.
AcidandBase/NeutralPollutantsColumn:PTETM-5capillarycolumn,30mx0.
25mmID,0.
25mfilmCat.
No.
:24135-UOven:35°C(4min)to300°Cat10°C/min,hold10minCarrier:helium,40cm/sec(setat250°C)Det.
:MS(massrange:35-450m/z;1.
18sec/scan)p-Chloro-m-cresolHexachlorocyclopentadiene2,4,6-Trichlorophenol2-ChloronaphthaleneAcenaphthyleneDimethylphthalate2,6-Dintrotoluene3.
Acenaphthene-d10Acenaphthene2,4-Dinitrophenol4-Nitrophenol2,4-DinitrotolueneDiethylphthalateFluorene4-Chlorophenylphenylether4,6-Dinitro-o-cresolN-Nitrosodiphenylamine1,2-Diphenylhyrazine4-BromophenylphenyletherHexachlorobenzenePentachlorophenol4.
Phenanthrene-d10PhenanthreneAnthraceneDi-n-butylphthalateFluorantheneBenzidinePyreneBenzylbutylphthalateBenzo(a)anthracene3,3'-Dichlorobenzidine5.
Chrysene-d12Chrysenebis(2-Ethylhexyl)phthalateDi-n-octylphthalateBenzo(b)fluorantheneBenzo(k)fluorantheneBenzo(a)pyrene6.
Indeno(1,2,3-cd)pyreneDibenzo(a,h)anthraceneBenzo(ghi)perylene123456797-0665100203040MinSUPELCOBulletin77510FigureT.
ChlorinatedPesticidesColumn:SPB-608capillarycolumn,30mx0.
25mmID,0.
25mfilmCat.
No.
:24103-UOven:150°C(4min)to290°Cat8°C/min,hold5minCarrier:helium,25cm/sec(setat290°C*)Det.
:ECDInj.
:0.
8Ldecanecontaining160pgeachanalyte,splitlessinjection,45secholdtime796-0056*AhalogenatedgassuchaschloromethaneorchlorotrifluoromethaneisusedtodeterminelinearvelocitybyECD.
04812162024Min12345678910111312141615WideBoreCapillaryColumnsAreCompatiblewithPurgeandTrapDevicesOur0.
53mmIDand0.
75mmIDVOCOLcolumnsarespecifi-callymanufacturedandtestedtorapidlyseparatethevolatilepollutantslistedinEPAMethods601,602,and624forwastewater(FigureU)and502.
2and524.
2fordrinkingwater.
Becausethesecolumnshavehighoptimumcarriergasflowrates,theyacceptsamplesdirectlyfrompurgeandtrapdevices.
VOCOLcolumnsarecompatiblewithlesssensitivedetectorsandconcentration-de-pendenthyphenatedtechniques,suchasGC-FTIR.
The0.
53mmIDcolumnshaveacapacityofabout2000ng/samplecomponent;the0.
75mmIDcolumnshaveacapacityofabout10,000ng/component.
FigureU.
VolatilePriorityPollutantsColumns:VOCOLcapillarycolumns,60mx0.
75mmID,1.
5mfilm(top)or30mx0.
53mmID,3.
0mfilmCat.
No.
:25320-U(bottom)Oven:10°C(6min)to170°Cat6°C/min(top)or35°C(4min)to190°Cat4°C/minCarrier:helium,10mL/minDet.
:FID1.
Dichlorodifluoromethane2.
Chloromethane3.
Vinylchloride4.
BromomethaneC.
Chloromethane(whenpresent)T.
Trichlorofluoromethane(whenpresent)5.
1,1-Dichloroethylene6.
Methylenechloride7.
trans-1,2-Dichloroethylene8.
1,1-Dichloroethane9.
cis-1,2-Dichloroethylene10.
Chloroform11.
Bromochloromethane12.
1,1,1-Trichloroethane13.
Carbontetrachloride14.
Benzene15.
1,2-Dichloroethane16.
Trichloroethylene17.
1,2-Dichloropropane18.
Bromodichloromethane19.
2-Chloroethylvinylether20.
cis-1,3-Dichloropropene21.
Toluene22.
trans-1,3-Dichloropropene23.
1-Chloro-2-bromopropane24.
1,1,2-Trichloroethane25.
Tetrachloroethylene26.
Dibromochloromethane27.
Chlorobenzene28.
Ethylbenzene29.
Bromoform30.
1,4-Dichlorobutane31.
1,1,2,2-Tetrachloroethane32.
1,3-Dichlorobenzene33.
1,4-Dichlorobenzene34.
1,2-Dichlorobenzene1234567891011121314151617181920232425262732303129282122322824201612840MinTC33341234567891011121314,151617181920232425262732303129282122333424201612840Min797-0653,540.
25mmIDand0.
53mmIDSPB-608capillarycolumnsofferexcellentresolvingpowerandinertnessforseparatingthepesti-cideslistedinUSEPAMethod608(FigureT).
Inadditiontoroutinetestingforefficiencyandinertness,eachSPB-608columnistestedtoensureminimalbreakdownofDDTandendrin,asrequiredbytheEPAmethod.
Furthermore,youcanusea0.
53mmIDcolumnininstrumentsdesignedforpackedcolumns.
1.
α-BHC2.
Lindane(γ-BHC)3.
β-BHC4.
Heptachlor5.
δ-BHC6.
Aldrin7.
Heptachlorepoxide8.
EndosulfanI9.
4,4'-DDE10.
Dieldrin11.
Endrin12.
4,4'-DDD13.
EndosulfanII14.
4,4'-DDT15.
Endrinaldehyde16.
Endosulfansulfate0.
75mmIDColumnSampledirectlytransferredfromapurgeandtrapdevice0.
53mmIDColumnSampledirectlyinjected11SUPELCOBulletin775OrderingInformation:PackingsandColumnsforUSEPAWastewaterAnalysesForchemicalstandardsforthesemethods,refertothecurrentSupelcocatalog.
EPAMethodNo.
ClassofPollutantsPackingsandColumnsCat.
No.
GasorLiquidChromatography601Purgeable60/80CarbopackB/1%SP-1000,15g11815HalocarbonsPackingforConfirmationColumn:n-Octaneon80/100PorasilC,75cc11733-UCapillaryGCColumns:VOCOL,60mx0.
75mmIDglassNA*VOCOL,30mx0.
53mmIDfusedsilica25320-U602Purgeable5%SP-1200/1.
75%Bentone34onAromatics100/120SUPELCOPORT,20g12134PackingforConfirmationColumn:5%1,2,3-TCEPon60/80ChromosorbWAW,20g11765-UCapillaryGCColumns:VOCOL,60mx0.
75mmIDglassNA*VOCOL,30mx0.
53mmIDfusedsilica25320-U603Acrolein&80/100PorapakQS,24g20343AcrylonitrilePackingforConfirmationColumn:80/100Chromosorb101,50g20214604PhenolsFreePhenols:1%SP-1240-DAon100/120SUPELCOPORT,20g11832Derivatives:5%SP-2250on80/100SUPELCOPORT,20g11737CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304605BenzidinesHPLCColumn:SUPELCOSILLC-1,25cmx4.
6mm58296CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304606Phthalates1.
5%SP-2250/1.
95%SP-2401on100/120SUPELCOPORT,20g11947PackingforConfirmationColumn:3%SP-2100on100/120SUPELCOPORT,20g11738CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304607Nitrosamines10%CARBOWAX20M/2%KOHon80/100ChromosorbWAW,20g11739CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304608Organochlorine1.
5%SP-2250/1.
95%SP-2401onPesticides&100/120SUPELCOPORT,20g11947PCBsPackingforConfirmationColumn:3%SP-2100on100/120SUPELCOPORT,20g11738CapillaryGCColumns:SPB-608,30mx0.
25mmIDfusedsilica24103-USPB-608,15mx0.
53mmIDfusedsilica25310-U609Nitroaromatics&1.
5%SP-2250/1.
95%SP-2401onIsophorone100/120SUPELCOPORT,20g11947PackingforConfirmationColumn:3%SP-2100on100/120SUPELCOPORT,20g11738CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304610PAHs3%SP-2250on100/120SUPELCOPORT,20g11744HPLCColumn:SUPELCOSILLC-PAH,25cmx4.
6mm58229CapillaryGCColumn:SPB-5,30mx0.
53mmIDfusedsilica25305-U611Haloethers3%SP-1000on100/120SUPELCOPORT,20g11746CapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica25304SUPELCOBulletin77512BULLETIN775BNVPackingsandColumnsforUSEPAWastewaterAnalyses(contd.
)EPAMethodNo.
ClassofPollutantsPackingsandColumnsCat.
No.
612Chlorinated1.
5%OV-1/2.
4%OV-225on80/100SUPELCOPORTcustomHydrocarbonsCapillaryGCColumn:SPB-5,15mx0.
53mmIDfusedsilica253046132,3,7,8-TCDDCapillaryGCColumns:SP-2331,60mx0.
25mmIDfusedsilica24104-USP-2331,60mx0.
32mmIDfusedsilica24105GC/MSMethods624Purgeable60/80CarbopackB/1%SP-1000,15g11815HalocarbonsPackingforConfirmationColumn:60/80CarbopackC/0.
2%CARBOWAX1500,15g11826PackingforPrecolumn:3%SP-1000on60/80ChromosorbWAW,20g11741CapillaryGCColumns:VOCOL,60mx0.
75mmIDglassNA*VOCOL,30mx0.
53mmIDfusedsilica25320-U625Acids(Phenols),3%SP-2250on100/120SUPELCOPORT,20g11756Base-Neutrals,Acids:Organochlorine1%SP-1240-DAon100/120SUPELCOPORT,20g11832Pesticides,Acids,Base-Neutrals,OrganochlorinePesticides,PCBs:PCBsPTE-5,30mx0.
25mmIDfusedsilica(0.
25mphasefilm)24135-U*Nolongeravailable;werecommenda0.
53mmIDfusedsilicacolumnforthisapplication.
Forchemicalstandardsforthesemethods,refertothecurrentSupelcocatalog.
References1.
SamplingandAnalysisProceduresforScreeningofIndustrialEffluentsforPriorityPollutantsUSEPAEffluentGuidelinesDivision,Washington(April,1977).
2.
FederalRegisterVol.
44,No.
233,Dec.
3,1979.
3.
FederalRegisterVol.
45,No.
98,May19,1980.
4.
Bellar,T.
A.
,andJ.
J.
Lichtenberg,J.
Am.
WaterWorksAssoc.
,66:739-744(1974).
5.
ManualofAnalyticalMethodsfortheAnalysisofPesticidesinHumanandEnvironmentalSamplesEPA-600/8-80-038,USEPA,HealthEffectsResearchLaboratory,ResearchTrianglePark,NC(1980).
ReferencesnotavailablefromSupelco.
TrademarksCarbopack,PTE,SP,SPB,SUPELCOPORT,SUPELCOSIL,VOCOL–Sigma-AldrichCo.
Bentone–NationalLeadCompany,BaroidSalesDivisionCARBOWAX–UnionCarbideCorporationChromosorb–CeliteCorp.
Durapak,Porapak,Porasil–WatersAssociates,Inc.
Florisil–U.
S.
SilicaCo.
Gas-Chrom–AppliedScienceLaboratories,Inc.
LiChrosorb–EMLaboratories,Inc.
OV–OhioValleySpecialtyChemicalCompanyTenax–EnkaResearchInstituteArnhemFusedsilicacolumnsmanufacturedunderHPUSpatentno.
4,293,415.
Formoreinformation,orcurrentprices,contactyournearestSupelcosubsidiarylistedbelow.
Toobtainfurthercontactinformation,visitourwebsite(www.
sigma-aldrich.
com),seetheSupelcocatalog,orcontactSupelco,Bellefonte,PA16823-0048USA.
ARGENTINA·Sigma-AldrichdeArgentina,S.
A.
·BuenosAires1119AUSTRALIA·Sigma-AldrichPty.
Ltd.
·CastleHillNSW2154AUSTRIA·Sigma-AldrichHandelsGmbH·A-1110WienBELGIUM·Sigma-AldrichN.
V.
/S.
A.
·B-2880BornemBRAZIL·Sigma-AldrichQuimicaBrasilLtda.
·01239-010SoPaulo,SPCANADA·Sigma-AldrichCanada,Ltd.
·2149WinstonParkDr.
,Oakville,ONL6H6J8CZECHREPUBLIC·Sigma-Aldrichs.
r.
o.
·18600Praha8DENMARK·Sigma-AldrichDenmarkA/S·DK-2665VallensbaekStrandFINLAND·Sigma-AldrichFinland/YA-KemiaOy·FIN-00700HelsinkiFRANCE·Sigma-AldrichChimie·38297Saint-Quentin-FallavierCedexGERMANY·Sigma-AldrichChemieGmbH·D-82041DeisenhofenGREECE·Sigma-Aldrich(o.
m.
)Ltd.
·Ilioupoli16346,AthensHUNGARY·Sigma-AldrichKft.
·H-1067BudapestINDIA·Sigma-AldrichCo.
·Bangalore560048IRELAND·Sigma-AldrichIrelandLtd.
·Dublin24ISRAEL·SigmaIsraelChemicalsLtd.
·Rehovot76100ITALY·Sigma-Aldrichs.
r.
l.
·20151MilanoJAPAN·Sigma-AldrichJapanK.
K.
·Chuo-ku,Tokyo103KOREA·Sigma-AldrichKorea·SeoulMALAYSIA·Sigma-Aldrich(M)Sdn.
Bhd.
·SelangorMEXICO·Sigma-AldrichQuímicaS.
A.
deC.
V.
·50200TolucaNETHERLANDS·Sigma-AldrichChemieBV·3330AAZwijndrechtNORWAY·Sigma-AldrichNorway·Torshov·N-0401OsloPOLAND·Sigma-AldrichSp.
zo.
o.
·61-663PoznaPORTUGAL·Sigma-AldrichQuimica,S.
A.
·Sintra2710RUSSIA·Sigma-AldrichRussia·Moscow103062SINGAPORE·Sigma-AldrichPte.
Ltd.
SOUTHAFRICA·Sigma-Aldrich(pty)Ltd.
·JetPark1459SPAIN·Sigma-AldrichQuimica,S.
A.
·28100Alcobendas,MadridSWEDEN·Sigma-AldrichSwedenAB·13570StockholmSWITZERLAND·Supelco·CH-9471BuchsUNITEDKINGDOM·Sigma-AldrichCompanyLtd.
·Poole,DorsetBH124QHUNITEDSTATES·Supelco·SupelcoPark·Bellefonte,PA16823-0048·Phone800-247-6628or814-359-3441·Fax800-447-3044or814-359-3044·email:supelco@sial.
comHSupelcoisamemberoftheSigma-Aldrichfamily.
SupelcoproductsaresoldthroughSigma-Aldrich,Inc.
Sigma-AldrichwarrantsthatitsproductsconformtotheinformationcontainedinthisandotherSigma-Aldrichpublications.
Purchasermustdeterminethesuitabilityoftheproductforaparticularuse.
Additionaltermsandconditionsmayapply.
Pleaseseethereversesideoftheinvoiceorpackingslip.