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SHORTREPORTOpenAccessFullsequenceanalysisandcharacterizationoftheSouthKoreanNorovirusGII-4variantCUK-3Jeong-WoongPark1,Sung-GeunLee2,Young-MinLee3,Weon-HwaJheong4,SangryeolRyu1*andSoon-YoungPaik2*AbstractBackground:Manyofresearchershavefocusedontheemergingpathogen,Norovirus,sinceitsfirstidentificationasthecausingagentofnonbacterialacutegastroenteritisinhumans.
Oneofthevirulencefactorsofnorovirus,thegreatgeneticdiversityattributedtopointmutationsandrecombinations,hasbroughtforththeresultofsignificantchangesinthecirculatingnorovirusgenotypepatterns.
Findings:Inrecognitionofthenecessityfortrackingandmonitoringofgeneticdiversity,anorovirusvariantamongthemostprevalentgenotypeGII-4,NorovirusHu/GII-4/CUK-3/2008/KR(CUK-3),wasisolatedfromstoolsamplesandanalyzedonthelevelofwholegenomesequence.
WholegenomesequenceanalysisrevealedthreeORFcompositesofthewholegenome,ORF1(5100bp),ORF2(1623bp),andORF3(807bp).
EachgeneticrelationshipofCUK-3variantanalysislocatedtheORF1(5,100bp)inClusterI,ORF2(1623bp)inClusterI(2006b),ORF3(807bp)inClusterI,andthewholegenomesequence(about5.
1kb)inClusterIinthephylogenetictree.
AndthephylogeneticanalysesshowedthesamelocationofCUK-3strainwiththeGII-4/2006bclusterinthephylogenetictree.
Conclusions:InThisstudy,afirstconcerningthefull-lengthsequenceofaNoVvariantinSouthKoreaismeaningfulinthatitcanbeusednotonlyasafull-lengthNoVvariantsequencestandardforfuturecomparisonstudies,butalsoasusefulmaterialforthepublichealthfieldbyenablingthediagnosis,vaccinedevelopment,andpredictionofnewemergingvariants.
Keywords:NorovirusSequence,PhylogenetictreeFindingsNoroviruses(NoVs)arethemostimportantvirusesthatcausenonbacterialacutegastroenteritisinhumans.
Inadditiontoincreasedsusceptibility,theelderlyareatincreasedriskformoreseverediseaseanddeath,asaretheveryyoungandtheimmunocompromised[1,2].
Theyaresmall,andnon-envelopedviruseswhichandbelongtothefamilyCaliciviridae,genusNorovirus.
Noroviruseshaveasinglepositive-strandNoVRNAgenomeofabout7.
6kbinsize.
Threeopenreadingframes(ORFs)havebeenidentifiedintheNoVgen-omes.
ORF1encodesapolyproteinthatiscleavedintosixnon-structural(NS)proteins,whichcarryaminoacidsequencemotifsconservedinNTPase,proteaseandRNA-dependentRNApolymerase(RdRp)[3,4].
ORF2encodesamajorstructuralprotein,ViralProtein(VP1),whichconsistoftwodomains-theshelldomain(S)andtheprotrudingarm(P)thatisagaindividedintotwosubdomains,P1andP2.
TheSdomainishighlycon-servedwhilethePdomainisvariable.
P2ofthePdomainishypervariableandcarriesimmuneandcellularrecognitionsites[5-7].
ORF3encodesminorcapsidpro-tein,VP2,whichisrichinbasicaminoacidsandispro-posedtohavearoleinviralstability[8,9]Recently,NoVswererecognizedasnovelemergentpathogens.
Themainrouteoftransmissionissuspectedtobeperson-to-person,butfoodandwater-borne*Correspondence:sangryu@snu.
ac.
kr;paik@catholic.
ac.
kr1DepartmentofFoodandAnimalBiotechnology,DepartmentofAgriculturalBiotechnology,CenterforAgriculturalBiomaterials,andResearchInstituteforAgricultureandLifeSciences,SeoulNationalUniversity,Seoul151-921,RepublicofKorea2DepartmentofMicrobiology,CollegeofMedicine,TheCatholicUniversityofKorea,Seoul137-701,RepublicofKoreaFulllistofauthorinformationisavailableattheendofthearticleParketal.
VirologyJournal2011,8:167http://www.
virologyj.
com/content/8/1/1672011Parketal;licenseeBioMedCentralLtd.
ThisisanOpenAccessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense(http://creativecommons.
org/licenses/by/2.
0),whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.
transmissionisalsoimportant[1,10,11].
Accordingtonucleotidesequenceanalysisofthecapsidregions,noro-virusesareclassifiedintofivegenogroups,GItoGV,eachofwhichcanbefurtherdividedintoseveralclus-tersorgenotypes[12].
Amongthefivegenogroups,threegenogroups(GI,GIIandGIV)areknowntocauseclinicalillnessinhumans,andgenotypeGII-4hasbeenthepredominantcirculatingstraintothepresent[13].
Theerror-proneRNAreplicationandrecombinationbetweenvirusesiswhatdrivesnorovirusestoitsthegreatdiversity.
Furthermore,theaccumulatedmutationsofthehypervariableP2domainoftheVP1proteinpro-duceddifferentGII-4NoVs[14].
Themostrepresenta-tiveoftheresultingvariantGII-4strain,GII-4/2006b,with3nucleotideinsertionsintheP2domainatposi-tion6265,emergedinthesummerof2002whichleadtoamajorgastroenteritisoutbreakaswellasanepi-demicgastroenteritisworldwideinthewinterof2002/2003[15,16].
InSouthKorea,gastroenteritisoutbreaksbyGII-4/2006bvariantshavebeenreportedfromSep-temberof2007toJulyof2008hadbeenreported[17].
Inthispaper,thewholegenomesequenceofanotherisolatedvariantoftheemergingstraintype,theGII-4variant,wasanalyzedandcomparedwithothervariantstorevealthegeneticrelationshipandtopredicttheten-dencyofGII-4variantsinSouthKorea.
NoVpositive-stoolsamplewasisolatedfrompatientswithacutegastroenteritisinDaejeon,SouthKoreainNovember2008.
ThesamplewasobtainedfromtheWaterborneVirusBank(Seoul,Korea).
Thestoolsamplewasstoredat-70°.
TheviralgenomicRNAwasextractedfrom140μlof10%fecalsuspensionwiththeQIAampViralRNAMinikit(Qiagen,Hilden,Germany)inaccor-dancewiththemanufacturer'sinstructions.
ForthedetectionofNoV,reversetranscriptionPCR(RT-PCR)wasperformedwiththeOneStepRT-PCRkit(Qiagen,Hilden,Germany)withprimersbasedonthesequenceofthenoroviruscapsidregion(Table1).
Toanalyzethewholegenomesequenceofthedetectednorovirusstrain,reversetranscriptionPCR(RT-PCR)wasperformedwiththeOneStepRT-PCRkit(Qiagen,Hilden,Germany)with10pairsofnewlydesignedprimersets(Table1).
Tenfragmentswereamplified;eightfragmentsforORF1,onefragmentforORF2,andonefragmentforORF3.
ThePCRproductswerethenanalyzedbyelectrophoresison1.
5%agarosegelandethidiumbromidestaining.
Theamplifiedfrag-mentswerepurifiedwithHiYieldGel/PCRDNAExtractionkit(RBC,Taipei,Taiwan)fromthegel.
Then,theproductswereclonedintothepGEM-TEasyvector(Promega,USA)andweresequencedbyGenotech(Dae-jeon,SouthKorea).
SequencedataanalysisofthecompositesequenceoftenplasmidsalignedwithClustalWmethodusingtheDNAStarsoftware(DNAStarInc.
)revealedthatthewholegenomewascomposedofthreeORFs,5100bp(ORF1),1623bp(ORF2),and807bp(ORF3).
Thedendrogramswereconstructedwiththeneighbor-joiningmethod.
ThenucleotidesequencedatareportedinthisstudyhavebeendepositedintoGenBank(acces-sionnumber;FJ514242).
AcomparisonofthenucleotideandaminoacidsequencesoftheentireORF2withreferencestrainsshowedthatCUK-3strainhadthehighestsequencesimilaritytotheNoVGII-4referencestrainX76717.
ThenucleotidesimilaritywithCUK-3was60.
1-88.
0%withthereferencestrains.
Aproteindatabasesearch(BLASTX)fortheproductofORF2yielded59.
3-92.
0%similarity.
However,thecut-offvalueforaminoacidsimilaritybetweentheCUK-3variantandtheGII-4referencestrainsshowedlowidentity(92.
0%)forthegenomicsequences(Table2).
ToanalyzethegeneticrelationshipbetweentheCUK-3variantandtheothervariantsreportedworldwide,thesequencesofORFsequencesandthewholegenomewereprocessedwithmultiplesequencealignment.
EachanalysisofCUK-3locatedtheORF1(5,100bp)inClus-terI,ORF2(1623bp)inClusterI(2006b),andORF3(807bp)inClusterIaswellasthewholegenomesequence(about5.
1kb)inClusterI(Figure1).
AsshownonthecaseanalysisofORF1,ClusterIcon-tainsHiroshoma1,Kumamoto1,Saga1,Hokkaodo1,Aomori4,Miayagi5,Akita1,Aichi3andshowedthehighestsimilarityrange(98.
0~98.
9%)(Figure1A).
AsshownonthecaseanalysisofORF2,ClusterI(2006b)containsemergedtheGII-4varintsinJapanandshowedthehighestsimilarityrange(97.
7-99.
0%),whereasClusterII(2006a)showedlowsimilarityrange(91.
7-92.
3%)(Figure1B).
AsshownonthecaseanalysisofORF3,ClusterIshowedthehighestsimilarityrange(97.
9-99.
3%).
Inter-estingly,02/03groupcontainsemergedtheGII-4vaiantsinEuropeshowedmorehighersimilarityrange(93.
1-93.
6%)thanClusterIIcontainsemergedtheGII-4variantsinJapan(Figure1C).
Asshownonthecaseanalysisoffulllength,theCUK-3strainshowedthehighestsimilarity(98.
3%)withthe2006epidemicstrain,Aichi3/2006/JPstrain,whereasEhime/5-30/2005/JPstrainandLordsdale/1993/UKstrainshowedthelowestsimilarity(89.
2%)(Figure1D).
TheCUK-3straincanbeplacedonthesamebranchofthephylogenetictreeastheGII-4/2006bcluster,basedonthenucleotidesequenceoftheRdRpregionandtheaminoacidsequenceofthecapsidprotein(datanotshown).
Also,theanalysisoftheGDDmotif(AANNTG)oftheRdRpregionoftheGII-4variantsreportedworldwideindicatedthattheGDDmotifofCUK-3strainwassimilartothatoftheGII-4variantsofParketal.
VirologyJournal2011,8:167http://www.
virologyj.
com/content/8/1/167Page2of5theepidemicstrainsin2006.
Thesestrainswerecom-moninHongKong,Japan,andotherregionsofNorthEastAsiaduringthesameperiod[12,18].
Suchsimilari-tiesinanalysesbetweenCUK-3andGII-4/2006bstrainareevidenceofthereemergenceofGII-4variant.
InEuropeandAsia,mostnorovirusoutbreaksthatoccurredafter2002havebeencausedbyGII-4variantstrains(Figure2).
Inaddition,biennialemergencesofpandemicNoVGII-4variantshavebeenreportedin2002,2004,2006and2008[12,18,19].
TheseGII-4var-iantshavealsobeenidentifiedinSouthKoreaandarerapidlyspreadingworldwide.
AminoacidsequencesofthecapsidregionencodedbyORF2wascomparedwithatotalof39GII-4variantsreportedfromvariouscountriesincludingUS,UK,Japan,China,Germanyetc.
Dateof540aminoacidanalysesshowedthepatternofchangesoftwoaminoacidinfirstP1domain,aa238(SGII-4->2002TGII-4-2002FGII-4-2002PGII-4-2002PGII-4-1995YGII-4->2002FGII-4-2002EGII-4-2006TGII-4-1995QGII-4->2002)andaa504(PGII-4->2002QGII-4-<2002).
Theseanalyticdatashowedthattherehadalreadybeennumerousmutations,whichleadtotheproductionofawiderangeofvariants,alreadybefore2002andpro-ducedvariousvariants.
Andasshownonthecaseofaa394ofP2domainwhichinsertednovelaminoacid,GorS,andthensubstitutedtoTafter2006(datanotshown).
AlthoughpreviousstudieshaveindicatedthatthesequencefortheNoVcapsidregionisusefulforthediag-nosisofNoVvariants,nostudieshaveanalyzedtheRdRpregionoftheSouthKoreanNoVGII-4variant.
Thisisthefirstreportthatdescribesthefull-lengthsequenceofaNoVGII-4variantthatwasisolatedfromclinicalsamplesinSouthKorea.
Wesuggestthatthissequencecanbeusedasastandardforthecomparisonoffull-lengthNoVGII-4variantsequencewithotherstrains.
TheinformationacquiredfromwholegenomesequencinginthisstudycanbeusefulnotonlyforamoreaccuratediagnosesofNoVsbutalsoforthebasicresearchfortheelucidationgeneticfunctions.
Further-more,itwillbehelpfulforthepredictionofnewlyappearingpandemicvariantsthroughcomparisonwithGII-4variantsinneighboringcountries,fundamentalresearchforvaccinedevelopment,andeventually,forthefieldofpublichealththroughwiththeprovisionofnewemergingstrainsofNoV.
AcknowledgementsThisworkwassupportedbyMid-careerResearcherProgram(2008-0061575)andBasicScienceResearchProgram(2009-0062720)throughNRFgrantfundedbytheMEST.
Authordetails1DepartmentofFoodandAnimalBiotechnology,DepartmentofAgriculturalBiotechnology,CenterforAgriculturalBiomaterials,andResearchInstituteforAgricultureandLifeSciences,SeoulNationalUniversity,Seoul151-921,RepublicofKorea.
2DepartmentofMicrobiology,CollegeofMedicine,TheCatholicUniversityofKorea,Seoul137-701,RepublicofKorea.
3DepartmentofMicrobiology,CollegeofMedicineandMedicalResearchInstitute,ChungbukNationalUniversity,Cheongju,Chungcheongbuk-do361-763,RepublicofKorea.
4EnvironmentalInfrastructureResearchDepartment,NationalInstituteofEnvironmentalResearch,Incheon,404-708,RepublicofKorea.
Authors'contributionsSRRandSYPconceivedthisstudy.
YML,WHJanddesignedandconductedtheexperiments.
SGLandJWPanalyzedthesequencedataandcarriedoutthemolecularphylogeneticanalysis.
SGLandSYPwrotethemanuscript.
Allauthorsreadandapprovedthefinalmanuscript.
CompetinginterestsTheauthorsdeclarethattheyhavenocompetinginterests.
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