Bsm1www.se998.com
www.se998.com 时间:2021-03-18 阅读:()
www.
evrogen.
com1pKillerRed-memvectorrestrictionmapThedatahasnotbeenverifiedbyrestrictiondigestionwitheachenzymelistedanddoesnottakeintoaccountpossiblemethylationsites.
Enzymesthatrecognizeunambiguoussequenceslessthan6basepairslongarenotincluded–forthemorecompleteenzymelistpleaserefertotheTableofrestrictionsites.
Uniquesitesareshowninbold-blue.
Thelocationgivenspecifiesthe3'endofthecutDNA(thebasetotheleftofthecutsite).
NeuromodulinN-terminal(mem)aminoacidsareshowninblack,KillerRedaminoacidsareshowninbold-red.
Ase1Bgl1||TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG1100ATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCAat2SpAccAat2|||CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGT101200GGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCABgl1Nde1Aat2Bgl1Bmr1AAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA201300TTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATBtgZ1SnaB1Nco1|||CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGA301400GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTAat2Eci1||TAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA401500ATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATwww.
evrogen.
com2Nhe1Afe1||ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTA501600TGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATBamH1Sma1|Apa1||PspOM|||Sac2||||Xho1Hind3EcoR1Sal1Kpn1|||||BpuE1Bgl2|Sac1|BstB1|Pst1|Acc65Age1CCGGACTCAGATCTCGAGCTCAAGCTTCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGTCGCCACCATGCTGTGCTGTATGAGAAGAA601700GGCCTGAGTCTAGAGCTCGAGTTCGAAGCTTAAGACGTCAGCTGCCATGGCGCCCGGGCCCTAGGTGGCCAGCGGTGGTACGACACGACATACTCTTCTTmem>MLCCMRRTBgl2|CCAAACAGGTTGAAAAGAATGATGAGGACCAAAAGATCTCCGAGGGCGGCCCCGCCCTGTTCCAGAGCGACATGACCTTCAAAATCTTCATCGACGGCGA701800GGTTTGTCCAACTTTTCTTACTACTCCTGGTTTTCTAGAGGCTCCCGCCGGGGCGGGACAAGGTCTCGCTGTACTGGAAGTTTTAGAAGTAGCTGCCGCTmem/KillerRed>KQVEKNDEDQKISEGGPALFQSDMTFKIFIDGEAle1Bsa1SpDonSpDon|ApaL1Dra3|GGTGAACGGCCAGAAGTTCACCATCGTGGCCGACGGCAGCAGCAAGTTCCCCCACGGCGACTTCAACGTGCACGCCGTGTGCGAGACCGGCAAGCTGCCC801900CCACTTGCCGGTCTTCAAGTGGTAGCACCGGCTGCCGTCGTCGTTCAAGGGGGTGCCGCTGAAGTTGCACGTGCGGCACACGCTCTGGCCGTTCGACGGGKillerRed>VNGQKFTIVADGSSKFPHGDFNVHAVCETGKLPwww.
evrogen.
com3PflM1|ATGAGCTGGAAGCCCATCTGCCACCTGATCCAGTACGGCGAGCCCTTCTTCGCCCGCTACCCCGACGGCATCAGCCATTTCGCCCAGGAGTGCTTCCCCG9011000TACTCGACCTTCGGGTAGACGGTGGACTAGGTCATGCCGCTCGGGAAGAAGCGGGCGATGGGGCTGCCGTAGTCGGTAAAGCGGGTCCTCACGAAGGGGCKillerRed>MSWKPICHLIQYGEPFFARYPDGISHFAQECFPEBspM1BfuA1Aar1Bpu10Ale1SpDon|||||AGGGCCTGAGCATCGACCGCACCGTGCGCTTCGAGAACGACGGCACCATGACCAGCCACCACACCTACGAGCTGGACGACACCTGCGTGGTGAGCCGCAT10011100TCCCGGACTCGTAGCTGGCGTGGCACGCGAAGCTCTTGCTGCCGTGGTACTGGTCGGTGGTGTGGATGCTCGACCTGCTGTGGACGCACCACTCGGCGTAKillerRed>GLSIDRTVRFENDGTMTSHHTYELDDTCVVSRI_ChiPflM1|Pvu2||Bsa1BspLUCACCGTGAACTGCGACGGCTTCCAGCCCGACGGCCCCATCATGCGCGACCAGCTGGTGGACATCCTGCCCAACGAGACCCACATGTTCCCCCACGGCCCC11011200GTGGCACTTGACGCTGCCGAAGGTCGGGCTGCCGGGGTAGTACGCGCTGGTCGACCACCTGTAGGACGGGTTGCTCTGGGTGTACAAGGGGGTGCCGGGGKillerRed>TVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPPvu2SpDon||AACGCCGTGCGCCAGCTGGCCTTCATCGGCTTCACCACCGCCGACGGCGGCCTGATGATGGGCCACTTCGACAGCAAGATGACCTTCAACGGCAGCCGCG12011300TTGCGGCACGCGGTCGACCGGAAGTAGCCGAAGTGGTGGCGGCTGCCGCCGGACTACTACCCGGTGAAGCTGTCGTTCTACTGGAAGTTGCCGTCGGCGCKillerRed>NAVRQLAFIGFTTADGGLMMGHFDSKMTFNGSRADra3Pml1||CCATCGAGATCCCCGGCCCACACTTCGTGACCATCATCACCAAGCAGATGAGGGACACCAGCGACAAGCGCGACCACGTGTGCCAGCGCGAGGTGGCCTA13011400GGTAGCTCTAGGGGCCGGGTGTGAAGCACTGGTAGTAGTGGTTCGTCTACTCCCTGTGGTCGCTGTTCGCGCTGGTGCACACGGTCGCGCTCCACCGGATKillerRed>IEIPGPHFVTIITKQMRDTSDKRDHVCQREVAYwww.
evrogen.
com4Not1Dra3Eag1Xba1BsaB1||||CGCCCACAGCGTGCCCCGCATCACCAGCGCCATCGGTAGCGACGAGGATTAAAGCGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGG14011500GCGGGTGTCGCACGGGGCGTAGTGGTCGCGGTAGCCATCGCTGCTCCTAATTTCGCCGGCGCTGAGATCTAGTATTAGTCGGTATGGTGTAAACATCTCCKillerRed>AHSVPRITSAIGSDED*Bsm1SpDonDra1Mfe1|Hpa1polyAPsi1TTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGG15011600AAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGTATTTTACTTACGTTAACAACAACAATTGAACAAATAACGTCGAATATTACCpolyApolyABts1Bsm1Afl2TTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAAGGC16011700AATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTCCGSpDonSsp1Psi1|||GTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTAT17011800CATTTAACATTCGCAATTATAAAACAATTTTAAGCGCAATTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATABsaXbDrd1BsaXa|||AAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA18011900TTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTBtgZ1Dra3|SpAcc|||CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG19012000GGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCwww.
evrogen.
com5Nae1NgoM4|BsrB1|||CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCG20012100GGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTT21012200CAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAABciV1BspH1|polyABsrB1||Ssp1Ear1Bsu36GTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTCCTGAGGCGGA22012300CAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCAGGACTCCGCCTNsi1Sph1|Eci1BfrB1|Pvu2|BseY1BstAP||AAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAA23012400TTCTTGGTCGACACCTTACACACAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTNsi1Sph1|BfrB1|SexA1BseY1BstAP||Eci1CCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCAT24012500GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAwww.
evrogen.
com6Eci1Sfi1Eci1Bmr1|Eci1Nco1polyABgl1CCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTA25012600GGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAvr2Stu1|BspM1SpDonBseR1||Cla1BsaB1BfuA1TTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGATCGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGA26012700AAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTCTAGCTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTEag1Bmr1||TTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGT27012800AACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACANar1Kas1|Drd1Msc1||||CAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGAC28012900GTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGPvu2Fsp1|PflF1Eco57||||GGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCAC29013000CCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGwww.
evrogen.
com7BtgZ1BspM1|SpDonBciV1BsrD1BfuA1|CTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATC30013100GAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGSap1Ear1BpuE1||GCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAG31013200CGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCSph1Nco1BtgZ1|||GCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTC32013300CGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGBseY1Nae1|Sap1NgoM4||Rsr2Eci1Ear1Eco57ATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCT33013400TAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGABtgZ1BssS1BsrB1|BsrB1BstB1TCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATG34013500AGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCGCCCTGAGACCCCAAGCTTTACwww.
evrogen.
com8BssS1Nae1BspM1NgoM4|BfuA1SpAccBpm1|||||ACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCT35013600TGGCTGGTTCGCTGCGGGTTGGACGGTAGTGCTCTAAAGCTAAGGTGGCGGCGGAAGATACTTTCCAACCCGAAGCCTTAGCAAAAGGCCCTGCGGCCGABpm1SpAcc|Avr2|||GGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCG36013700CCTACTAGGAGGTCGCGCCCCTAGAGTACGACCTCAAGAAGCGGGTGGGATCCCCCTCCGATTGACTTTGTGCCTTCCTCTGTTATGGCCTTCCTTGGGCpolyApolyA||CGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACC37013800GCGATACTGCCGTTATTTTTCTGTCTTATTTTGCGTGCCACAACCCAGCAAACAAGTATTTGCGCCCCAAGCCAGGGTCCCGACCGTGAGACAGCTATGGBsa1|CCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCG38013900GGTGGCTCTGGGGTAACCCCGGTTATGCGGGCGCAAAGAAGGAAAAGGGGTGGGGTGGGGGGTTCAAGCCCACTTCCGGGTCCCGAGCGTCGGTTGCAGCBstAPAlwN1Bsu36Dra1Dra1||||GGGCGGCAGGCCCTGCCATAGCCTCAGGTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTT39014000CCCGCCGTCCGGGACGGTATCGGAGTCCAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAABspH1|TGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTT40014100ACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAwww.
evrogen.
com9BpuE1Eco57||CTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG41014200GACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACSpAcc|GCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCT42014300CGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGAAlwN1BpuE1||GCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGC43014400CGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGApaL1BseY1SpAcc|||TGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCG44014500ACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCBciV1Eci1|BssS1SpAcc||||AAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCC45014600TTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGDrd1BpuE1SpAccEci1||||TGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGG46014700ACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCwww.
evrogen.
com10SpDonNsi1BspLU|BfrB1|||||TTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCATGCAT47014787AAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGTACGTAFound:Aar1Aat2Acc65Afe1Afl2Age1Ale1AlwN1Apa1ApaL1Ase1Avr2BamH1BciV1BfrB1BfuA1Bgl1Bgl2Bmr1Bpm1Bpu10BpuE1Bsa1BsaB1BsaXaBsaXbBseR1BseY1Bsm1BspH1BspLUBspM1BsrB1BsrD1BssS1BstAPBstB1Bsu36BtgZ1Bts1_ChiCla1Dra1Dra3Drd1Eag1Ear1Eci1Eco57EcoR1Fsp1Hind3Hpa1Kas1Kpn1Mfe1Msc1Nae1Nar1Nco1Nde1NgoM4Nhe1Not1Nsi1PflF1PflM1Pml1polyAPsi1PspOMPst1Pvu2Rsr2Sac1Sac2Sal1Sap1SexA1Sfi1Sma1SnaB1SpAccSpDonSph1Ssp1Stu1Xba1Xho1Unique:Aar1Acc65Afe1Afl2Age1Apa1Ase1BamH1Bpu10BsaXaBsaXbBseR1BsrD1Bts1_ChiCla1EcoR1Fsp1Hind3Hpa1Kas1Kpn1Mfe1Msc1Nar1Nde1Nhe1Not1PflF1Pml1PspOMPst1Rsr2Sac1Sac2Sal1SexA1Sfi1Sma1SnaB1Stu1Xba1Xho1www.
evrogen.
com11Notfound:Acl1Ahd1Asc1AsiS1Bae1aBae1bBbs1BbvC1Bcg1aBcg1bBcl1Blp1BmgB1Bsg1BsiW1BsmB1BspE1BsrG1BssH2BstE2BstX1BstZ1EcoKEcoN1EcoRVScFRTFse1FspA1I_CeuloxPMlu1Nru1Pac1Pme1PshA1Pvu1SanD1Sbf1Sca1Sgf1SgrA1Spe1Srf1Swa1T3RNAT7RNAT7TerPISceXcm1Xmn1Excludedbysitecomplexity:Acc1Aci1Afl3Alu1Alw1Apo1Ava1Ava2Ban1Ban2Bbv1BceA1Bfa1Bme15BsaA1BsaH1BsaJ1BsaW1BseM2BsiE1BsiH1Bsl1BsmA1BsmF1Bsp12BspCaBspCbBsr1BsrF1BssK1BstF5BstN1BstU1BstY1Btg1Cac8CviJ1Dde1Eae1EcoO1Fau1Fnu4HFok1Hae2Hae3Hga1Hha1Hinc2Hinf1HinP1Hpa2Hph1Hpy99Hpy1Hpy3HpyC3HpyC4HpyC5Mae3Mbo2Mnl1Mse1Msl1MspA1Mwo1Nci1Nla3Nla4Nsp1Ple1PpuM1Rsa1Sau3ASau96SfaN1Sfc1Sml1Sty1Taq1Tat1Tfi1Tse1Tsp45Tsp50TspR1
evrogen.
com1pKillerRed-memvectorrestrictionmapThedatahasnotbeenverifiedbyrestrictiondigestionwitheachenzymelistedanddoesnottakeintoaccountpossiblemethylationsites.
Enzymesthatrecognizeunambiguoussequenceslessthan6basepairslongarenotincluded–forthemorecompleteenzymelistpleaserefertotheTableofrestrictionsites.
Uniquesitesareshowninbold-blue.
Thelocationgivenspecifiesthe3'endofthecutDNA(thebasetotheleftofthecutsite).
NeuromodulinN-terminal(mem)aminoacidsareshowninblack,KillerRedaminoacidsareshowninbold-red.
Ase1Bgl1||TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG1100ATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCAat2SpAccAat2|||CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGT101200GGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCABgl1Nde1Aat2Bgl1Bmr1AAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA201300TTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATBtgZ1SnaB1Nco1|||CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGA301400GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTAat2Eci1||TAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA401500ATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATwww.
evrogen.
com2Nhe1Afe1||ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTA501600TGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATBamH1Sma1|Apa1||PspOM|||Sac2||||Xho1Hind3EcoR1Sal1Kpn1|||||BpuE1Bgl2|Sac1|BstB1|Pst1|Acc65Age1CCGGACTCAGATCTCGAGCTCAAGCTTCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGTCGCCACCATGCTGTGCTGTATGAGAAGAA601700GGCCTGAGTCTAGAGCTCGAGTTCGAAGCTTAAGACGTCAGCTGCCATGGCGCCCGGGCCCTAGGTGGCCAGCGGTGGTACGACACGACATACTCTTCTTmem>MLCCMRRTBgl2|CCAAACAGGTTGAAAAGAATGATGAGGACCAAAAGATCTCCGAGGGCGGCCCCGCCCTGTTCCAGAGCGACATGACCTTCAAAATCTTCATCGACGGCGA701800GGTTTGTCCAACTTTTCTTACTACTCCTGGTTTTCTAGAGGCTCCCGCCGGGGCGGGACAAGGTCTCGCTGTACTGGAAGTTTTAGAAGTAGCTGCCGCTmem/KillerRed>KQVEKNDEDQKISEGGPALFQSDMTFKIFIDGEAle1Bsa1SpDonSpDon|ApaL1Dra3|GGTGAACGGCCAGAAGTTCACCATCGTGGCCGACGGCAGCAGCAAGTTCCCCCACGGCGACTTCAACGTGCACGCCGTGTGCGAGACCGGCAAGCTGCCC801900CCACTTGCCGGTCTTCAAGTGGTAGCACCGGCTGCCGTCGTCGTTCAAGGGGGTGCCGCTGAAGTTGCACGTGCGGCACACGCTCTGGCCGTTCGACGGGKillerRed>VNGQKFTIVADGSSKFPHGDFNVHAVCETGKLPwww.
evrogen.
com3PflM1|ATGAGCTGGAAGCCCATCTGCCACCTGATCCAGTACGGCGAGCCCTTCTTCGCCCGCTACCCCGACGGCATCAGCCATTTCGCCCAGGAGTGCTTCCCCG9011000TACTCGACCTTCGGGTAGACGGTGGACTAGGTCATGCCGCTCGGGAAGAAGCGGGCGATGGGGCTGCCGTAGTCGGTAAAGCGGGTCCTCACGAAGGGGCKillerRed>MSWKPICHLIQYGEPFFARYPDGISHFAQECFPEBspM1BfuA1Aar1Bpu10Ale1SpDon|||||AGGGCCTGAGCATCGACCGCACCGTGCGCTTCGAGAACGACGGCACCATGACCAGCCACCACACCTACGAGCTGGACGACACCTGCGTGGTGAGCCGCAT10011100TCCCGGACTCGTAGCTGGCGTGGCACGCGAAGCTCTTGCTGCCGTGGTACTGGTCGGTGGTGTGGATGCTCGACCTGCTGTGGACGCACCACTCGGCGTAKillerRed>GLSIDRTVRFENDGTMTSHHTYELDDTCVVSRI_ChiPflM1|Pvu2||Bsa1BspLUCACCGTGAACTGCGACGGCTTCCAGCCCGACGGCCCCATCATGCGCGACCAGCTGGTGGACATCCTGCCCAACGAGACCCACATGTTCCCCCACGGCCCC11011200GTGGCACTTGACGCTGCCGAAGGTCGGGCTGCCGGGGTAGTACGCGCTGGTCGACCACCTGTAGGACGGGTTGCTCTGGGTGTACAAGGGGGTGCCGGGGKillerRed>TVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPPvu2SpDon||AACGCCGTGCGCCAGCTGGCCTTCATCGGCTTCACCACCGCCGACGGCGGCCTGATGATGGGCCACTTCGACAGCAAGATGACCTTCAACGGCAGCCGCG12011300TTGCGGCACGCGGTCGACCGGAAGTAGCCGAAGTGGTGGCGGCTGCCGCCGGACTACTACCCGGTGAAGCTGTCGTTCTACTGGAAGTTGCCGTCGGCGCKillerRed>NAVRQLAFIGFTTADGGLMMGHFDSKMTFNGSRADra3Pml1||CCATCGAGATCCCCGGCCCACACTTCGTGACCATCATCACCAAGCAGATGAGGGACACCAGCGACAAGCGCGACCACGTGTGCCAGCGCGAGGTGGCCTA13011400GGTAGCTCTAGGGGCCGGGTGTGAAGCACTGGTAGTAGTGGTTCGTCTACTCCCTGTGGTCGCTGTTCGCGCTGGTGCACACGGTCGCGCTCCACCGGATKillerRed>IEIPGPHFVTIITKQMRDTSDKRDHVCQREVAYwww.
evrogen.
com4Not1Dra3Eag1Xba1BsaB1||||CGCCCACAGCGTGCCCCGCATCACCAGCGCCATCGGTAGCGACGAGGATTAAAGCGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGG14011500GCGGGTGTCGCACGGGGCGTAGTGGTCGCGGTAGCCATCGCTGCTCCTAATTTCGCCGGCGCTGAGATCTAGTATTAGTCGGTATGGTGTAAACATCTCCKillerRed>AHSVPRITSAIGSDED*Bsm1SpDonDra1Mfe1|Hpa1polyAPsi1TTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGG15011600AAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGTATTTTACTTACGTTAACAACAACAATTGAACAAATAACGTCGAATATTACCpolyApolyABts1Bsm1Afl2TTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAAGGC16011700AATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTCCGSpDonSsp1Psi1|||GTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTAT17011800CATTTAACATTCGCAATTATAAAACAATTTTAAGCGCAATTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATABsaXbDrd1BsaXa|||AAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA18011900TTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTBtgZ1Dra3|SpAcc|||CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG19012000GGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCwww.
evrogen.
com5Nae1NgoM4|BsrB1|||CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCG20012100GGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTT21012200CAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAABciV1BspH1|polyABsrB1||Ssp1Ear1Bsu36GTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTCCTGAGGCGGA22012300CAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCAGGACTCCGCCTNsi1Sph1|Eci1BfrB1|Pvu2|BseY1BstAP||AAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAA23012400TTCTTGGTCGACACCTTACACACAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTNsi1Sph1|BfrB1|SexA1BseY1BstAP||Eci1CCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCAT24012500GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAwww.
evrogen.
com6Eci1Sfi1Eci1Bmr1|Eci1Nco1polyABgl1CCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTA25012600GGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAvr2Stu1|BspM1SpDonBseR1||Cla1BsaB1BfuA1TTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGATCGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGA26012700AAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTCTAGCTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTEag1Bmr1||TTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGT27012800AACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACANar1Kas1|Drd1Msc1||||CAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGAC28012900GTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGPvu2Fsp1|PflF1Eco57||||GGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCAC29013000CCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGwww.
evrogen.
com7BtgZ1BspM1|SpDonBciV1BsrD1BfuA1|CTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATC30013100GAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGSap1Ear1BpuE1||GCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAG31013200CGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCSph1Nco1BtgZ1|||GCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTC32013300CGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGBseY1Nae1|Sap1NgoM4||Rsr2Eci1Ear1Eco57ATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCT33013400TAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGABtgZ1BssS1BsrB1|BsrB1BstB1TCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATG34013500AGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCGCCCTGAGACCCCAAGCTTTACwww.
evrogen.
com8BssS1Nae1BspM1NgoM4|BfuA1SpAccBpm1|||||ACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCT35013600TGGCTGGTTCGCTGCGGGTTGGACGGTAGTGCTCTAAAGCTAAGGTGGCGGCGGAAGATACTTTCCAACCCGAAGCCTTAGCAAAAGGCCCTGCGGCCGABpm1SpAcc|Avr2|||GGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCG36013700CCTACTAGGAGGTCGCGCCCCTAGAGTACGACCTCAAGAAGCGGGTGGGATCCCCCTCCGATTGACTTTGTGCCTTCCTCTGTTATGGCCTTCCTTGGGCpolyApolyA||CGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACC37013800GCGATACTGCCGTTATTTTTCTGTCTTATTTTGCGTGCCACAACCCAGCAAACAAGTATTTGCGCCCCAAGCCAGGGTCCCGACCGTGAGACAGCTATGGBsa1|CCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCG38013900GGTGGCTCTGGGGTAACCCCGGTTATGCGGGCGCAAAGAAGGAAAAGGGGTGGGGTGGGGGGTTCAAGCCCACTTCCGGGTCCCGAGCGTCGGTTGCAGCBstAPAlwN1Bsu36Dra1Dra1||||GGGCGGCAGGCCCTGCCATAGCCTCAGGTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTT39014000CCCGCCGTCCGGGACGGTATCGGAGTCCAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAABspH1|TGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTT40014100ACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAwww.
evrogen.
com9BpuE1Eco57||CTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG41014200GACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACSpAcc|GCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCT42014300CGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGAAlwN1BpuE1||GCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGC43014400CGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGApaL1BseY1SpAcc|||TGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCG44014500ACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCBciV1Eci1|BssS1SpAcc||||AAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCC45014600TTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGDrd1BpuE1SpAccEci1||||TGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGG46014700ACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCwww.
evrogen.
com10SpDonNsi1BspLU|BfrB1|||||TTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCATGCAT47014787AAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGTACGTAFound:Aar1Aat2Acc65Afe1Afl2Age1Ale1AlwN1Apa1ApaL1Ase1Avr2BamH1BciV1BfrB1BfuA1Bgl1Bgl2Bmr1Bpm1Bpu10BpuE1Bsa1BsaB1BsaXaBsaXbBseR1BseY1Bsm1BspH1BspLUBspM1BsrB1BsrD1BssS1BstAPBstB1Bsu36BtgZ1Bts1_ChiCla1Dra1Dra3Drd1Eag1Ear1Eci1Eco57EcoR1Fsp1Hind3Hpa1Kas1Kpn1Mfe1Msc1Nae1Nar1Nco1Nde1NgoM4Nhe1Not1Nsi1PflF1PflM1Pml1polyAPsi1PspOMPst1Pvu2Rsr2Sac1Sac2Sal1Sap1SexA1Sfi1Sma1SnaB1SpAccSpDonSph1Ssp1Stu1Xba1Xho1Unique:Aar1Acc65Afe1Afl2Age1Apa1Ase1BamH1Bpu10BsaXaBsaXbBseR1BsrD1Bts1_ChiCla1EcoR1Fsp1Hind3Hpa1Kas1Kpn1Mfe1Msc1Nar1Nde1Nhe1Not1PflF1Pml1PspOMPst1Rsr2Sac1Sac2Sal1SexA1Sfi1Sma1SnaB1Stu1Xba1Xho1www.
evrogen.
com11Notfound:Acl1Ahd1Asc1AsiS1Bae1aBae1bBbs1BbvC1Bcg1aBcg1bBcl1Blp1BmgB1Bsg1BsiW1BsmB1BspE1BsrG1BssH2BstE2BstX1BstZ1EcoKEcoN1EcoRVScFRTFse1FspA1I_CeuloxPMlu1Nru1Pac1Pme1PshA1Pvu1SanD1Sbf1Sca1Sgf1SgrA1Spe1Srf1Swa1T3RNAT7RNAT7TerPISceXcm1Xmn1Excludedbysitecomplexity:Acc1Aci1Afl3Alu1Alw1Apo1Ava1Ava2Ban1Ban2Bbv1BceA1Bfa1Bme15BsaA1BsaH1BsaJ1BsaW1BseM2BsiE1BsiH1Bsl1BsmA1BsmF1Bsp12BspCaBspCbBsr1BsrF1BssK1BstF5BstN1BstU1BstY1Btg1Cac8CviJ1Dde1Eae1EcoO1Fau1Fnu4HFok1Hae2Hae3Hga1Hha1Hinc2Hinf1HinP1Hpa2Hph1Hpy99Hpy1Hpy3HpyC3HpyC4HpyC5Mae3Mbo2Mnl1Mse1Msl1MspA1Mwo1Nci1Nla3Nla4Nsp1Ple1PpuM1Rsa1Sau3ASau96SfaN1Sfc1Sml1Sty1Taq1Tat1Tfi1Tse1Tsp45Tsp50TspR1
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