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HumanOMgp/OMGProtein,HisTagCatalog#OMG-H5228www.
acrobiosystems.
com11/20/2019SynonymOMG,OMGPSourceHumanOMgp,HisTag(OMG-H5228)isexpressedfromhuman293cells(HEK293).
ItcontainsAAIle25-Asn420(Accession#AAH18050).
PredictedN-terminus:Ile25MolecularCharacterizationThisproteincarriesapolyhistidinetagattheC-terminus.
TheproteinhasacalculatedMWof45.
5kDa.
Theproteinmigratesas90-120kDaunderreducing(R)condition(SDS-PAGE)duetoglycosylation.
EndotoxinLessthan1.
0EUperμgbytheLALmethod.
Purity>92%asdeterminedbySDS-PAGE.
FormulationLyophilizedfrom0.
22μmfilteredsolutioninPBS,pH7.
4.
Normallytrehaloseisaddedasprotectantbeforelyophilization.
Contactusforcustomizedproductformorformulation.
ReconstitutionPleaseseeCertificateofAnalysisforspecificinstructions.
Forbestperformance,westronglyrecommendyoutofollowthereconstitutionprotocolprovidedintheCoA.
StorageForlongtermstorage,theproductshouldbestoredatlyophilizedstateat-20°Corlower.
Pleaseavoidrepeatedfreeze-thawcycles.
Noactivitylosswasobservedafterstorageat:-20°Cto-70°Cfor12monthsinlyophilizedstate;-70°Cfor3monthsundersterileconditionsafterreconstitution.
SDS-PAGEHumanOMgp,HisTagonSDS-PAGEunderreducing(R)condition.
ThegelwasstainedovernightwithCoomassieBlue.
Thepurityoftheproteinisgreaterthan92%.
BackgroundOligodendrocyte-myelinglycoprotein(OMGorOMGP),isacellmembraneproteinwhichcontainseightLRR(leucine-rich)repeatsandoneLRRNTdomain.
OMG/OMGPisaglycosylphosphatidylinositol-anchoredproteinexpressedbyneuronsandoligodendrocytesinthecentralnervoussystem(CNS).
OMG/OMGPplayrolesinboththedevelopingandadultcentralnervoussystem.
OMG/OMGPmayalsobeinvolvedintheformationandmaintenanceofmyelinsheaths.
References(1)MikolD.
D.
,etal.
,1990,J.
CellBiol.
110:471-479.
HumanOMgp/OMGProtein,HisTagCatalog#OMG-H5228www.
acrobiosystems.
com11/20/2019(2)MikolD.
D.
,etal.
,1988,J.
CellBiol.
106:1273-1279.
PleasecontactusviaTechSupport@acrobiosystems.
comifyouhaveanyquestiononthisproduct.
acrobiosystems.
com11/20/2019SynonymOMG,OMGPSourceHumanOMgp,HisTag(OMG-H5228)isexpressedfromhuman293cells(HEK293).
ItcontainsAAIle25-Asn420(Accession#AAH18050).
PredictedN-terminus:Ile25MolecularCharacterizationThisproteincarriesapolyhistidinetagattheC-terminus.
TheproteinhasacalculatedMWof45.
5kDa.
Theproteinmigratesas90-120kDaunderreducing(R)condition(SDS-PAGE)duetoglycosylation.
EndotoxinLessthan1.
0EUperμgbytheLALmethod.
Purity>92%asdeterminedbySDS-PAGE.
FormulationLyophilizedfrom0.
22μmfilteredsolutioninPBS,pH7.
4.
Normallytrehaloseisaddedasprotectantbeforelyophilization.
Contactusforcustomizedproductformorformulation.
ReconstitutionPleaseseeCertificateofAnalysisforspecificinstructions.
Forbestperformance,westronglyrecommendyoutofollowthereconstitutionprotocolprovidedintheCoA.
StorageForlongtermstorage,theproductshouldbestoredatlyophilizedstateat-20°Corlower.
Pleaseavoidrepeatedfreeze-thawcycles.
Noactivitylosswasobservedafterstorageat:-20°Cto-70°Cfor12monthsinlyophilizedstate;-70°Cfor3monthsundersterileconditionsafterreconstitution.
SDS-PAGEHumanOMgp,HisTagonSDS-PAGEunderreducing(R)condition.
ThegelwasstainedovernightwithCoomassieBlue.
Thepurityoftheproteinisgreaterthan92%.
BackgroundOligodendrocyte-myelinglycoprotein(OMGorOMGP),isacellmembraneproteinwhichcontainseightLRR(leucine-rich)repeatsandoneLRRNTdomain.
OMG/OMGPisaglycosylphosphatidylinositol-anchoredproteinexpressedbyneuronsandoligodendrocytesinthecentralnervoussystem(CNS).
OMG/OMGPplayrolesinboththedevelopingandadultcentralnervoussystem.
OMG/OMGPmayalsobeinvolvedintheformationandmaintenanceofmyelinsheaths.
References(1)MikolD.
D.
,etal.
,1990,J.
CellBiol.
110:471-479.
HumanOMgp/OMGProtein,HisTagCatalog#OMG-H5228www.
acrobiosystems.
com11/20/2019(2)MikolD.
D.
,etal.
,1988,J.
CellBiol.
106:1273-1279.
PleasecontactusviaTechSupport@acrobiosystems.
comifyouhaveanyquestiononthisproduct.
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