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Received:3December2001Accepted:6May2002Publishedonline:23July2002Springer-Verlag2002Aneditorialregardingthisarticlecanbefoundinthesameissue(http://dx.
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org/10.
1007/s00134-002-1428-x)AbstractObjective:Todeterminethetherapeuticefficacyandsafetyofplasmapheresisinthetreatmentofpatientswithseveresepsisandsepticshock.
Design:Prospective,random-ised,clinicaltrialwithaplanned,midstudy,interimanalysis.
Setting:Intensivecareunitinauni-versityhospitalinArchangels,Rus-sia.
Patients:Consecutivepatientswithseveresepsisorsepticshock.
Interventions:Onehundredandsixpatientswererandomisedtoreceiveeitherstandardtherapyoranadd-ontreatmentwithplasmapheresis.
Measurementsandresults:Thepri-maryendpointwas28-daysurvival.
Septicshockwasdiagnosedin57%oftheplasmapheresis-treatedpa-tientsand54%ofthecontrolpa-tients.
MeanAPACHEIIIscoreatentrywas56.
4intheplasmapheresisgroupand53.
5inthecontrolgroup.
The28-day,all-causemortalityratewas33.
3%(18/54)intheplasma-pheresisgroupand53.
8%(28/52)inthecontrolgroup.
Thisrepresentsarelativeriskforfataloutcomeintheplasmapheresisgroupof0.
61,anab-soluteriskreductionof20.
5%andanumberofpatientsneededtotreatof4.
9.
Apartfromsixtransientepi-sodesofhypotensionandonealler-gicreactiontofreshfrozenplasma,noadversereactionswereattribut-abletotheplasmapheresistreatmentinthisstudy.
Conclusions:Plasma-pheresismaybeanimportantadju-vanttoconventionaltreatmenttore-ducemortalityinpatientswithse-veresepsisorsepticshock.
Plasma-pheresisisasafeprocedureinthetreatmentofsepticpatients.
Apro-spectiverandomisedmulticentretrialiswarrantedtoconfirmourresultsandtodeterminewhichsubgroupsofsepticpatientswillbenefitmostfromthistreatmentmodality.
KeywordsAcutePhysiologyandChronicHealthEvaluation·Randomisedcontrolledtrial·Plasmapheresis·Sepsis·Septicshock·OutcomeIntensiveCareMed(2002)28:1434–1439DOI10.
1007/s00134-002-1410-7ORIGINALRolfBusundVladimirKouklineUriUtrobinEdvardNedashkovskyPlasmapheresisinseveresepsisandsepticshock:aprospective,randomised,controlledtrialIntroductionSepsisisanincreasinglycommoncauseofmorbidityandmortality,particularlyinelderly,immunocompro-misedandcriticallyillpatients[1].
Estimatedmortalityfromseveresepsisandsepticshockrangesfrom20%to60%[2,3].
Sepsisthereforerepresentstheleadingcauseofdeathinintensivecareunits[4,5],andtheincidencewillprobablycontinuetorisebecauseofdemographictrendsandincreaseduseofimmunosuppressiveagents,broad-spectrumantibioticsandinvasivetechnology.
Inrecentyearsmanynewtherapiesforsepsishavebeentestedinrandomisedclinicaltrials.
Acommonconceptoftheseinnovativetherapiesistheattempttocounteractthephysiologicalresponsetosepsismediatorsbyadmin-istrationofspecificantibodies,inhibitorsandantagonistsdirectedagainstthesemediators.
However,mostoftheseinnovativetherapieshavefailedtohaveaneffectonmortality[6].
Duringsepsis,particularlyGram-negativesepsis,theentirespectrumofhosteffectormoleculesareR.
Busund()DepartmentofCardiothoracicandVascularSurgery,TromsUniversityHospital,9038Troms,Norwaye-mail:rbusund@online.
noV.
Koukline·U.
UtrobinDepartmentofExtracorporalMethods,CityEmergencyHospital#1,Archangels,RussiaE.
NedashkovskyDepartmentofAnaesthesiologyandIntensiveCare,CityEmergencyHospital#1,Archangels,Russiareleased,manyofwhichhavebeenconfirmedtobere-sponsiblefortheclinicalsyndromeofsepsis.
Thissug-geststhatwhileblockingordown-regulatinganysinglemediatormaymodifyoratleastpartiallyabrogatetheorgandysfunctionseeninsepsis,itishighlyunlikelythatanysinglemodulatoryregimentargettingonesinglemediatorwouldbesuccessfulinreducingmortalityinaclinicalsettingofseveresepsisorsepticshock.
Further-more,manyofthemediatorsofsepsisareprobablyyetundiscovered,andourknowledgeofthemediatorsthathavebeendiscoveredisfarfromcomplete.
Plasmapheresisisanon-selectivemethodwiththepo-tentialtoremoveharmfulortoxicmediatorsfromthecirculation.
Usingfresh-frozenplasmaasreplacementfluid,consumedplasmafactorsaresubstituted,therebypossiblyrestoringtheopsoniccapacityandimprovingthecoagulationabnormalities,bothofwhicharedis-turbedinsepsis.
Since1979severalreportshavebeenpublishedonplasmapheresisandwholebloodexchangeforsepsis[7,8,9,10,11,12,13,14,15,16].
Thesere-ports,basedonasmallnumberofpatientsandwithoutappropriatecontrolpatients,areinconclusiveandcon-flictingwithrespecttowhetherplasmapheresisprovidesanybeneficialeffectsinthetreatmentofsepsis.
Conflict-ingresultsarealsoreportedinanimalstudies,whichhavesoughttoevaluatetheefficacyofplasmapheresisinsepsisandsepticshock[17,18,19].
InArchangelsplasmapheresishasbeenusedinthetreatmentofsepsisformanyyears,butthemethodhasnotbeenevaluatedbyaproperclinicaltrial.
Theaimofthepresentstudywastodeterminethetherapeuticeffica-cyandsafetyofplasmapheresisinthetreatmentofpa-tientswithseveresepsisandsepticshockinaprospec-tive,randomised,controlledtrial.
MaterialsandmethodsTheprotocolwasapprovedbytheinstitutionalreviewboardattheCityEmergencyHospital#1.
Informedconsentwasobtainedfromallconsciouspatientsenrolledinthestudy.
Delayedconsentwasobtainedfromsurvivingpatientswhowereunconsciousatthetimeofenrolment.
PatientselectionanddefinitionsEligiblepatientswereagedbetween17and70yearsandhadse-veresepsisorsepticshock.
SepsiswasdiagnosedaccordingtothecriteriaproposedbyBoneetal.
[20].
Thesystemicinflammatoryresponsetoinfectionincludedmorethanoneofthefollowingclinicalmanifestations;temperaturehigherthan38°Corlowerthan36°C,tachycardia(heartratehigherthan90beatspermin),tachypnoea(respiratoryratemorethan20breathspermin)orhy-perventilation(PaCO2lessthan4.
2kPa),andleukocytosis(whitebloodcellcountgreaterthan12,000/mm3)orleucopenia(whitebloodcellcountlessthan4000/mm3).
Severesepsiswasdefinedassepsisassociatedwithorgandysfunction,hypoperfusionabnor-mality,orsepsisinducedhypotension(systolicbloodpressurelessthan90mmHg).
Hypoperfusionmarkersusedwerelacticacidosis,oliguria,andalterationinmentalstatus.
Septicshockwasdefinedassepsis-inducedhypotension,persistingdespiteadequatefluidresuscitation,alongwiththepresenceofhypoperfusionabnormal-itiesororgandysfunction.
Patentswithhypoperfusionabnormali-tiesororgandysfunctionreceivinginotropicsupportwereconsid-eredtohavesepticshockeveniftheyhadnormalbloodpressures.
Patientstreatedforseveresepsisorsepticshockinotherhospitalsformorethan12hbeforetheyweretransferredtoCityHospital#1,andpatientswithsevereunderlyingdiseasewerenotincludedinthestudy.
Severeunderlyingdiseaseincludespatientswithter-minalcancer,terminalcardiacfailure,end-stagerenalfailureandpotentiallylethalinjuries.
Randomisation,treatmentproceduresandendpointAssoonasthediagnosisofseveresepsisorsepticshockwases-tablished,thepatientswererandomisedtoreceiveplasmapheresisinadditiontoconventionalsepsistreatmentorconventionalsepsistreatmentalone.
Thepatientswereblock-randomisedintwostagesallowinganinterimanalysisafterinclusionof50patients.
Allpa-tientsreceivedconventionalsepsistreatmentaccordingtotheindi-cationineachcase.
Thistreatmentincludedantibiotics,fluidre-suscitation(plasma,colloids,and/orcrystalloid),surgicalproce-dures,andcardiovascularandventilatorysupportwhenindicated.
Combinationtherapyofantibioticswaschosenaccordingtosourceofinfectionandmicro-organismssuspectedtobeinvolved,andcorrectedaccordingtopositivebacteriologicalcultureandre-sistancepatternswhenavailable.
Patientsinbothgroupswhodidnothavecontraindicationstoanticoagulationtherapyreceivedheparin.
Activatedpartialthromboplastintime,usedtomonitortheanticoagulationtherapy,waskeptbelow80sexceptduringtheperiodswhenpatientswereundergoingplasmapheresis.
Plasma-pheresiswasinitiatedwithin6hafterthediagnosiswasestab-lished.
Itwasrepeatedoncewithin24hin27patientsinwhomtheclinicalconditiondidnotimprove,orinwhomtheclinicalconditionstilldeterioratedafterthefirstprocedureasjudgedbythepresenceorprogressionofhaemodynamicinstabilityandthedevelopmentoforgandysfunction.
PlasmapheresiswasperformedemployingaPF-0.
5(Lvov,Russia),andaDK2-0.
3(Rjazan,Rus-sia)continuousflowplasmapheresismachinesusingveno-venousaccess.
Heparindosesof200–300U/kgbodyweightwasusedasanticoagulant.
Activatedclottingtimewaskeptbetween250and300s.
duringplasmapheresis.
Duringeachexchangesessionavol-umeof30–40ml/kgbodyweightofpatient'splasmawasex-changedwithanequalvolumeoffresh-frozenplasmafromheal-thydonors,dilutedwithanequalvolumeof5%humanalbuminsolution.
Thedurationofthefirstplasmapheresissessionwas133±23minandthesecondsession137±21min.
Themeanex-changeplasmavolumeduringthefirstsessionwas1820±402mland1763±312mlduringthesecondsession.
Thepatientswerefollowedfor28daysoruntiltheydied.
ForcomparisonofdiseaseseveritytheAcutePhysiologyandChronicHealthEvaluation(APACHE)IIIscorewascalculatedatstudyen-try,after24handafter48h[21].
Primaryendpointwas28-daysurvival.
PatientcharacteristicsThestudyincluded106consecutivepatients(60men,and46women;meanage44±15years)treatedbetweenDecember1994andMarch1997.
Atstudyentry56%ofthepatientswereinsepticshock(31/54intheplasmapheresisgrouppatientsand28/52inthecontrolgroup).
MeanbaselineAPACHEIIIscorewas54.
9±17.
4(56.
4intheplasmapheresisgroupand53.
5inthecontrolgroup).
Thelargestnumberofinfectionsoriginatedintheabdomen,with33patientsintheplasmapheresisgroupand16inthecontrolgroup.
Thenextlargestgrouphadrespiratorytractinfections14351436(n=12)followedbyurinarytractinfections(n=10).
Exceptforageanddistributionofsitesofinfection,therewerenostatisticallysignificantdifferencesbetweenthegroupswithrespecttothesebaselinecharacteristics(Table1).
Surgicalprocedureswereper-formedon65%(35/54)ofplasmapheresistreatedpatientsandon73%(38/52)ofcontrolpatients.
Inotropeswereusedin56%(30/54)ofpatientsintheplasmapheresisgroupandin52%(27/52)ofpatientsinthecontrolgroup.
Mechanicalventilationwasrequiredby46%intheplasmapheresisgroupandby67%inthecontrolgroup(Table2).
StatisticalanalysisStatisticalanalysiswasperformedusingSPSSforWindows,ver-sion10.
0(SPSS,Chicago,Ill.
,USA).
Univariatecomparisonsofbaselinecharacteristicsweremadebyunpairedttestforcontinu-ousvariablesandFisher'sexacttestforcategoricalvariables.
Pearson'sχ2wasusedtotestdifferencesininfectiousoriginbe-tweenthetwogroups.
ChangesinAPACHEIIIscorefrombase-linevalueswereassessedbypairedttest.
Fisher'sexacttestwasusedtotestdifferencesinsurvivalbetweenthegroups.
Multiplelogisticregressionwasusedtoassesstheeffectofthetreatmentvariableandthebaselinedemographicandprognosticvariablesonsurvival.
Thereportedmortalityratesrepresentallcausemortalityinthetwogroups,andtheanalysiswascompletedonanintentionto-treat-basis.
Dataarepresentedasmean±SD.
Differenceswereconsideredsignificantatpvalueslessthan0.
05.
Allreportedpvaluesaretwo-sided.
ResultsEfficacyandsafetyofplasmapheresisTheinterimanalysisrevealedamortalityof14/25inthecontrolgroupcomparedto8/25intheplasmapheresisgroup(n.
s.
).
Consequentlythestudywascontinued.
Nofataladversereactionswereattributabletotheplasma-pheresisprocedureinthisstudy.
Sixpatientshadshortandundramaticperiodsofhypotensionduringtheplas-mapheresisprocedure,andonepatienthadanallergicre-actiontofresh-frozenplasma.
Twopatientsintheplas-mapheresisgroupdiedofbleeding.
Onepatientwithme-diastinitisduetoastabwoundthatpenetratedtheab-dominalaorta,theomentumandthelowerpartoftheoe-sophagusdiedofmassiverebleedingfromtheabdominalaorta4daysafterhislastplasmapheresisprocedure.
Thesecondpatientthatdiedfrombleedinghadahaemor-rhagicpancreatitisanddied17daysaftertheplasma-pheresisprocedure.
Thusnoneofthemcouldberelatedtotheplasmapheresisprocedure.
Duringthefirst24hAPACHEIIIscoredecreasedby20%intheplasmapher-esisgroup(pAPCHEIIIscoredroppedinbothgroupsfromday1today2.
However,thechangeinAPACHEIIIFig.
1Cumulativesurvivalin106patientswithseveresepsisorsepticshockrandomlyassignedtoplasmapheresis(solidline)ornot(dottedline)inadditiontostandardsepsistreatmentTable3Prognosticscore,outcomeandcauseofdeathin106pa-tientswithseveresepsisorsepticshockrandomlyassignedtoplasmapheresisornotinadditiontostandardsepsistreatment(n.
a.
notassessed,ARDSadultrespiratorydistresssyndrome,DICdis-seminatedintravascularcoagulation)VariableaPlasmapheresisControlp(n=54)(n=52)MeanAPACHEIIIscoreDay156.
4±18.
853.
5±15.
80.
40Day244.
5±18.
5*49.
0±19.
70.
24Differencedays1–211.
5±15.
64.
5±15.
70.
0328-daymortalityTotalstudypopulation18(33.
3%)28(53.
8%)0.
05Abdominalgroup11/33(33.
3%)11/16(68.
5%)0.
03Othergroups7/21(33.
3%)17/36(47.
2%)0.
4CauseofdeathRespiratoryfailure/ARDS24n.
a.
Cardiovascular710n.
a.
Multiorganfailure613n.
a.
Brainherniation01n.
a.
DIC10n.
a.
Bleeding20n.
a.
aNumberofpatientsexceptwherestatedotherwise*p<0.
001vs.
baselineTable4Multiplelogisticregressionanalysisevaluatingthead-justedeffectsofunbalancedbaselinecharacteristicsandplasma-pheresisonmortalityinpatientswithseveresepsisorsepticshockIndependentvariableOddsratio95%CIpAge(10years)a1.
481.
03–2.
120.
03Siteofinfection0.
04AbdominalReferenceFemalegenital0.
540.
07–4.
00Urological0.
150.
02–0.
93Lung4.
040.
74–22.
2Skin/softtissue0.
410.
07–2.
53Brain1.
600.
30–8.
62Other1.
710.
33–8.
88Plasmaexchange0.
410.
15–1.
090.
07aDenotesoddsratiobyincreaseof10yearsscorefromday1today2wassignificantlybetterintheplasmapheresisgroup.
TheAPACHEscorehasbeenshowntobeareliablepredictorofoutcomeincriticallyillpatientsingeneral[24]aswellasinpatientswithsur-gicalandpostoperativeintra-abdominalinfections[25,26].
MeanAPACHEIIIscoreinourserieswas54.
9(17.
4)withacorrespondingoverallmortalityof43%,whichissomewhathigherthanwouldbeexpectedformtheAPACHEscoreifwecompareourmaterialwithpre-viousreports[24].
However,internationalcomparisonsmaybebiasedbydifferencesinlaboratorytests,differ-encesinpatientpopulationsandcaseselectionsforICUtreatment.
AsthismayinfluencethecalibrationoftheAPACHEestimates[27],themostusefulandreliablees-timatetodeterminethepatient'sresponsetotherapyistherelativetrendorchangeinAPACHEscorefromonedaytothenext(Table3).
ThisconceptissupportedbyKnausetal.
[21],whostatethatchangesintheAPACHEIIIscoreoneachsubsequentdayofICUtherapyprovidedailyupdatesintheriskestimates.
Bothclinicalandexperimentalstudieshaveshownthatplasmapheresislowerscirculatinglevelsofendo-toxinandcytokinessuchastumornecrosisfactorαandinterleukin1β[10,18,28,29,30].
Mostauthorsclaimthatthebeneficialeffectofplasmapheresisisduetotheremovalofthesemediators.
However,thebeneficialef-fectofplasmapheresisisprobablynotexplainedsolelybytheremovaloftoxicmediators.
Usingfresh-frozenplasmaasreplacementfluid,theprocedurealsoreplen-ishesdeficienciessuchastheimmunoglobulinsIgMandIgA[11]andcoagulationfactorsandinhibitorssuchasproteinsCandSandantitrombinIII.
Plasmapheresismaythusrestorecoagulationabnormalitiesandimproveopsoniccapacityandserumbactericidalactivity.
Thismayleadtoenhancementofthehumoralandcellularin-flammatoryresponseandnormalisationofDICandclot-tingparameters[7,9,10].
Supportforthisisgiveninplacebo-controlledtrialswhichhavetestedsupplemen-talimmunoglobulintherapyonpatientswithpostopera-tivesepsisandsepticshock[31].
Theroleofanticoagu-lationtherapyinsepsisisstillunsettled,andwecannotruleoutthepossibilitythattheadditionalheparindeliv-eredduringtheplasmapheresisprocedureaffectedout-come;however,thiscanberesolvedonlyinfuturetri-als.
UsingtheratherbroadsepsisdefinitionproposedbyBoneetal.
[20]asinclusioncriteria,weobtainedahet-erogeneousstudypopulationincludingpatientswithbothGram-negativeandGram-positivesepsisofvariousori-gins.
Aposthocanalysisinthegroupofpatientswithabdominalsepsis(comprising46%oftheoverallstudypopulation)revealedasignificantlyhighersurvivalrateintheplasmapheresisgroupthaninthecontrolgroup,whilethedifferenceinsurvivalfortherestofthestudypopulationwasnotsignificant.
ThebacterialspecieswiththegreatestpotentialforinvasivenessinabdominalsepsisareconsideredtobetheGram-negativebacilliEscherichiacoliandBacteroidesfragilis[32].
Thema-jorityofsepticpatientssuccessfullytreatedbyplasma-pheresishavesufferedfromsystemicmeningococcaldis-easeorotherGram-negativesepsis[7,8,10,12,13,16].
Mostoftheexperimentalstudiesinfavourofplasma-pheresishavebeenperformedonanimalschallengedwithGram-negativebacilliorendotoxin.
Ourdatadem-onstratingimprovedsurvivalinpatientswithabdominalsepsisfollowingplasmapheresisareinlinewiththesepreviousreportsandsuggestthatthebeneficialeffectofplasmapheresisinsepticpatientsmaybelimitedtopa-tientswithGram-negativesepsis.
Duetonegativebacteriologicalculturesormissingvaluesonmorethanone-halfofthepatients,wewerenotabletoconfirmthattheantibioticsgivenhadanyef-fectonthecausativemicro-organism.
Studieshaveshownthattreatmentwithappropriateantibioticsmayreduceshockandmortalityrateby50%[33].
Itisun-likely,however,thatthereisanydifferenceinresistancepatternbetweenthetwogroupswhobothhavebeentreatedwithcombinationtherapyofbroad-spectrumanti-bioticsinaccordancewiththelocaltraditions.
Further-more,accordingtoAstizandRackow[5],bacteraemiaoccursonlyin40–60%ofpatientswithsepticshock,andthecausativeorganismisnotisolatedin10–30%ofpa-tients,possiblybecauseofpreviousexposuretoantibiot-ics.
Thepresenteddataonmechanicalventilationrepre-sentsventilatortreatmentafterrandomisation.
Aswedonothavethebaselinedataonmechanicalventilation,wecannotruleoutthepossibilitythatdifferencesinme-chanicalventilationbetweenthetwogroupsmayhaveaffectedoutcome.
Thisisunlikely,however,sincethebaselineAPACHEIIIscorefortherespiratoryvariablesrespiratoryrateandPaO2werenotsignificantlydifferentbetweenthegroups.
Inconclusion,ourstudyshowsthatplasmapheresiscanbeperformedsafelyinpatientswithseveresepsisorsepticshock.
Thedataalsosupportthehypothesisthatplasmapheresisreducesmortalityinthesepatients,al-thoughunbalancedbaselinecharacteristicspreventsusfrommakinggeneralrecommendationsbasedonthisstudy.
Alargerscale,phaseIII,prospectiverandomisedmulticentretrialisneededtoconfirmourresultsandtodeterminewhichgroupsofsepticpatientswillparticular-lybenefitfromthistreatmentmodality.
AcknowledgementsWethankJanWilscow,MD,foradviceonstatisticalproceduresandProf.
DagG.
SrlieandProf.
MadsGilbertforhelpfulcomments.
GordonS.
Doig,PhD,BiostatisticalSupportUnit,ResearchOffice,RoyalNorthShoreHospital,Syd-ney,providedadditionalcommentsonpresentation,analysisandinterpretationinthefinalmanuscript.
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