target888ye.com
888ye.com 时间:2021-04-09 阅读:()
www.
lucigen.
comMA266E-RibonucleaseR,E.
coliFeb20191RibonucleaseR,E.
coliCat.
No.
RNR072502www.
lucigen.
com1.
IntroductionRibonucleaseR(RNaseR)fromE.
coli,isamagnesium-dependent3′→5′exoribonucleasethatdigestsessentiallyalllinearRNAsbutdoesnotdigestlariatorcircularRNAstructures,ordouble-strandedRNAwith3′-overhangsshorterthan7nucleotides.
1,2MostcellularRNAswillbedigestedcompletelybyRNaseR,withtheexceptionoftRNAs,5SRNAandintronlariats.
The3′-tailsoflariatswillbetrimmedbyRNaseRtothebranchpointnucleotide,wherethereisa2′,5′-phosphodiesterlinkage.
Lariatsareproducedduringpre-mRNAsplicingofintronregionsandcanbeisolatedfromamixtureoftotalRNAbydigestionwithRNaseR.
TheMasterPureCompleteDNAandRNAPurificationKitandMasterPureYeastRNAPurificationKitsareidealforsuchtotalRNApreparations.
RNAisolatedinthiswaycanbeusedasatemplatetoproducelabeledcDNAwhichisthenusedasatargetformicroarrayscontainingpotentialintronsequencesorfortilingarrayscontainingoverlappingregionsofcompletechromosomesorgenomes.
ThecDNAproducedinthiswaywillnotbealinearrepresentationoftheintron,butthesequencescontainedinitwillbeintron-derived.
RNaseRisprovidedasa250Usize(20U/L;1μg/L)andissuppliedwitha10XRNaseRReactionBuffer.
2.
ProductDesignationsandKitComponentsProductKitSizeCatalogNumberReagentDescriptionPartNumbersVolumeRNaseR250UnitsRNR07250RibonucleaseR(20U/L)E0111-20D112.
5L10XRNaseRReactionBufferSS000769-D1250L3.
ProductSpecificationsStorage:Storeonlyat-20°Cinafreezerwithoutadefrostcycle.
StorageBuffer:RNaseRissuppliedina50%glycerolsolutioncontaining50mMTris-HCl(pH7.
5),100mMNaCl,0.
1mMEDTA,0.
1%TritonX-100and1mMdithiothreitol.
UnitDefinition:Oneunitconverts1μgofpoly-r(A)intoacid-solublenucleotidesin10minutesat37°Cin20mMTris-HCl(pH8.
0),100mMKCland0.
1mMMgCl2.
10XRNaseRReactionBuffer:is:0.
2MTris-HCl(pH8.
0),1MKCland1mMMgCl2.
Note:RNaseRrequireslow(0.
1-1.
0mM)magnesiumconcentrationsforactivity.
LowEDTAconcentrationsinsubstrateRNAsolutionscannegativelyaffectRNaseRactivity.
AdditionalMgCl2,upto1mMfinalconcentrationcanbeusedtocompensateforEDTAinthesubstrate.
Optimalactivityisat37°C.
QualityControl:RNaseRisfunction-testedinareactioncontainingamixtureoflinearandcircularizedRNAoligonucleotides.
OnlythelinearRNAisdigested.
ContaminatingActivityAssays:RNaseRisfreeofdetectableendoribonucleaseandDNaseactivities.
techsupport@lucigen.
com888-575-969534.
ApplicationsAlternativesplicingstudiesGeneexpressionstudiesIntroncDNAproductionIntronicscreeningofcDNAlibrariesIsolationofsplicingintermediatesandlariats5.
References1.
Suzuki,H.
etal.
,(2006)Nucl.
AcidsRes.
34(8)e63.
2.
Vincent,H.
A.
andDeutscher,M.
P.
,(2006)J.
Biol.
Chem.
281(40)29769.
EpicentreisatrademarkofIllumina,Inc.
and/oritsaffiliate(s)intheU.
S.
andothercountries,andisusedunderlicense.
MasterPureisatrademarkofLucigen.
TritonisaregisteredtrademarkofRohm&Haas,Philadelphia,Pennsylvania.
lucigen.
comMA266E-RibonucleaseR,E.
coliFeb20191RibonucleaseR,E.
coliCat.
No.
RNR072502www.
lucigen.
com1.
IntroductionRibonucleaseR(RNaseR)fromE.
coli,isamagnesium-dependent3′→5′exoribonucleasethatdigestsessentiallyalllinearRNAsbutdoesnotdigestlariatorcircularRNAstructures,ordouble-strandedRNAwith3′-overhangsshorterthan7nucleotides.
1,2MostcellularRNAswillbedigestedcompletelybyRNaseR,withtheexceptionoftRNAs,5SRNAandintronlariats.
The3′-tailsoflariatswillbetrimmedbyRNaseRtothebranchpointnucleotide,wherethereisa2′,5′-phosphodiesterlinkage.
Lariatsareproducedduringpre-mRNAsplicingofintronregionsandcanbeisolatedfromamixtureoftotalRNAbydigestionwithRNaseR.
TheMasterPureCompleteDNAandRNAPurificationKitandMasterPureYeastRNAPurificationKitsareidealforsuchtotalRNApreparations.
RNAisolatedinthiswaycanbeusedasatemplatetoproducelabeledcDNAwhichisthenusedasatargetformicroarrayscontainingpotentialintronsequencesorfortilingarrayscontainingoverlappingregionsofcompletechromosomesorgenomes.
ThecDNAproducedinthiswaywillnotbealinearrepresentationoftheintron,butthesequencescontainedinitwillbeintron-derived.
RNaseRisprovidedasa250Usize(20U/L;1μg/L)andissuppliedwitha10XRNaseRReactionBuffer.
2.
ProductDesignationsandKitComponentsProductKitSizeCatalogNumberReagentDescriptionPartNumbersVolumeRNaseR250UnitsRNR07250RibonucleaseR(20U/L)E0111-20D112.
5L10XRNaseRReactionBufferSS000769-D1250L3.
ProductSpecificationsStorage:Storeonlyat-20°Cinafreezerwithoutadefrostcycle.
StorageBuffer:RNaseRissuppliedina50%glycerolsolutioncontaining50mMTris-HCl(pH7.
5),100mMNaCl,0.
1mMEDTA,0.
1%TritonX-100and1mMdithiothreitol.
UnitDefinition:Oneunitconverts1μgofpoly-r(A)intoacid-solublenucleotidesin10minutesat37°Cin20mMTris-HCl(pH8.
0),100mMKCland0.
1mMMgCl2.
10XRNaseRReactionBuffer:is:0.
2MTris-HCl(pH8.
0),1MKCland1mMMgCl2.
Note:RNaseRrequireslow(0.
1-1.
0mM)magnesiumconcentrationsforactivity.
LowEDTAconcentrationsinsubstrateRNAsolutionscannegativelyaffectRNaseRactivity.
AdditionalMgCl2,upto1mMfinalconcentrationcanbeusedtocompensateforEDTAinthesubstrate.
Optimalactivityisat37°C.
QualityControl:RNaseRisfunction-testedinareactioncontainingamixtureoflinearandcircularizedRNAoligonucleotides.
OnlythelinearRNAisdigested.
ContaminatingActivityAssays:RNaseRisfreeofdetectableendoribonucleaseandDNaseactivities.
techsupport@lucigen.
com888-575-969534.
ApplicationsAlternativesplicingstudiesGeneexpressionstudiesIntroncDNAproductionIntronicscreeningofcDNAlibrariesIsolationofsplicingintermediatesandlariats5.
References1.
Suzuki,H.
etal.
,(2006)Nucl.
AcidsRes.
34(8)e63.
2.
Vincent,H.
A.
andDeutscher,M.
P.
,(2006)J.
Biol.
Chem.
281(40)29769.
EpicentreisatrademarkofIllumina,Inc.
and/oritsaffiliate(s)intheU.
S.
andothercountries,andisusedunderlicense.
MasterPureisatrademarkofLucigen.
TritonisaregisteredtrademarkofRohm&Haas,Philadelphia,Pennsylvania.
- target888ye.com相关文档
- borne888ye.com
- chlorine888ye.com
- 南方基金管理股份有限公司关于民族证券并入方正证券期间基金业务安排的提示性公告
- establish888ye.com
- Output888ye.com
- panels888ye.com
gcorelabs：美国GPU服务器，8张RTX2080Ti，2*Silver-4214/256G内存/1T SSD/
gcorelabs提供美国阿什本数据中心的GPU服务器（显卡服务器），默认给8路RTX2080Ti，服务器网卡支持2*10Gbps（ANX），CPU为双路Silver-4214（24核48线程），256G内存，1Gbps独享带宽仅需150欧元、10bps带宽仅需600欧元，不限流量随便跑吧。 官方网站 ：https://gcorelabs.com/hosting/dedicated/gpu/ ...
易探云美国云服务器评测，主机低至33元/月,336元/年
美国服务器哪家平台好?美国服务器无需备案，即开即用，上线快。美国服务器多数带防御，且有时候项目运营的时候，防御能力是用户考虑的重点，特别是网站容易受到攻击的行业。现在有那么多美国一年服务器，哪家的美国云服务器好呢？美国服务器用哪家好?这里推荐易探云，有美国BGP、美国CN2、美国高防、美国GIA等云服务器，线路优化的不错。易探云刚好就是做香港及美国云服务器的主要商家之一，我们来看一下易探云美国云服...
GigsGigsCloud 春节优惠2022 指定云服务器VPS主机85折循环优惠码
GigsGigsCloud商家在之前介绍的还是比较多的，因为之前我一直有几台机器在使用，只是最近几年网站都陆续转型删除掉不少的网站和闲置域名，包括今年也都减少网站开始转型自媒体方向。GigsGigsCloud 商家产品还是比较有特色的，有提供香港、新加坡等亚洲机房的云服务器、VPS和独立服务器等。第一、新春优惠活动优惠码：CNY2022-15OFF截止到正月初二，我们可以使用上述优惠码在购买指定G...
888ye.com为你推荐
-
网易网盘关闭入口网易网盘怎么用????openeuler谁知道open opened close closed的区别吗kaixin.com人人网和开心网互通，可我用的是kaixin001的开心，和kaixin*com不是一个呀！李子柒年入1.6亿魔兽rpg箱庭世界1.6怎么进入魔门原代码源代码是什么rawtools闪迪32Gsd卡，无法格式化，显示只有30M，并且是raw格式。如何恢复？haole018.com为什么www.haole008.com在我这里打不开啊,是不是haole008换新的地址了?mole.61.com摩尔庄园的米米号和密码我都忘了 只记得注册的邮箱 怎么办-_-www.vtigu.com如图，已知四边形ABCD是平行四边形，下列条件：①AC=BD,②AB=AD，③∠1=∠2④AB⊥BC中，能说明平行四边形www.dm8.cc有谁知道海贼王最新漫画网址是多少？？