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第二军医大学学报AcadJSecMilMedUniv2005Feb;26(2)盐酸赈甲醋的经皮渗透特性研究俞旋陈攻张智艳高中l\丁雪鹰l189·告公著·(1.
第二军医大学药学院药剂学教研室,上海200433;2长海医院药学部)[摘要]町的:考察盐酸吸甲醋的体外经皮渗透特性.
方战:采用Franz扩散池,裸鼠离体皮肤作为透皮模型.
HPLC法测定药物透皮浓度口计算药物在不同浓度(10-100mgml-I)条件下经皮渗透稳态流量、渗透系数以及透皮时滞,并考察几种促透剂(氮町、\1-甲基-2-咣咯烧酬、丙二醇、油酸)对吸甲醋透皮行为的影响.
结果:盐酸吸甲醋的经皮渗透稳态流量随浓度增加显著增强(PO.
O日,而随着浓度增加(注50mg.
ml-I),肌1PH渗透系数有一定的提高CPO.
0日,但是其增渗比小于8%Azo肘,推测与PG增加了MPH在Azone中的榕解度从而阻止药物向皮肤角质层扩散有关.
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178(3)121-128[收稿日期]2004-09-21[本文编辑]尹茶[11嗲回日期]2004-11-29一←--+--←'EpitopeDNAvaccinesagainsttuberculosis:spacersandubiquitinmodulatescellularimmuneresponseselicitedbyepitopeDNAvaccineWangQM.
SunSH.
HuZL.
ZhouFJ.
YinM.
XiaoCJ.
ZhangJC(DepartmentofMedicalGenetics.
SecondMilitaryMedicalUniversity.
Shanghai200433.
China)[ABSTRACTJCell-mediatedimmuneresponsesarecrucialintheprotectionagainsttuberculosis.
1nthisstudy.
weconstructedepitopeD\lAvaccines(p3-M-38)encodingcytotoxicTlymphocyte(CTL)epitopesofMPT64and38kDaproteinsofMycobacreriumtuberculosis.
1nordertoobservetheinfluenceo[spacersequence(Ala-Ala-Tyr)orubiqu>tin(UbGR)ontheefficacyofthetwoCTLepitopes.
wealsoconstructedD:-iAvaccines.
p3-M-S(spacer)-38.
p3-Ub(UbGR)-M-S-38andp3-UbM-38.
TheimmuneresponseselicitedbythefourD\lAvaccinesweretestedinC57BL/6(H-2b)mice.
ThecytotoxicityofTcellswasdetectedbyLDH-releasemethodandbyenzyme-Iinkedimmunospotassayforepitope-specificcellssecretinginterferon-gamma.
TheresultsshowedthatD\lAimmunizationwithp3-M-38vaccinecouldinduceepitope-specificCD8+CTLresponseandthatthespacersequence(AAY)onlyenhancedMepitopepresentation.
Theprotein-targetingsequence(UbGR)enhancedtheimmunogenicityofthetwoepitopes.
ThefindingthatdefinedspacersequencesatC-terminusandprotein-targetingdegradationmodulatedtheimmuneresponseofepitopestringD\lAvaccineswillbeofimportanceforthefurtherdevelopmentofmulti-epitopeDNAvaccinesagainsttuberculosis.
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60(3):219-225J