Efcientwww.idanmu.com
www.idanmu.com 时间:2021-03-19 阅读:()
METHODSINMOLECULARBIOLOGYSeriesEditorJohnM.
WalkerSchoolofLifeandMedicalSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForfurthervolumes:http://www.
springer.
com/series/7651HumanMonoclonalAntibodiesMethodsandProtocolsSecondEditionEditedbyMichaelSteinitzDepartmentofPathology,TheHebrewUniversity,HadassahMedicalSchool,Jerusalem,IsraelEditorMichaelSteinitzDepartmentofPathologyTheHebrewUniversityHadassahMedicalSchoolJerusalem,IsraelISSN1064-3745ISSN1940-6029(electronic)MethodsinMolecularBiologyISBN978-1-4939-8957-7ISBN978-1-4939-8958-4(eBook)https://doi.
org/10.
1007/978-1-4939-8958-4LibraryofCongressControlNumber:2018964050SpringerScience+BusinessMedia,LLC,partofSpringerNature2019Thisworkissubjecttocopyright.
AllrightsarereservedbythePublisher,whetherthewholeorpartofthematerialisconcerned,specicallytherightsoftranslation,reprinting,reuseofillustrations,recitation,broadcasting,reproductiononmicrolmsorinanyotherphysicalway,andtransmissionorinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdeveloped.
Theuseofgeneraldescriptivenames,registerednames,trademarks,servicemarks,etc.
inthispublicationdoesnotimply,evenintheabsenceofaspecicstatement,thatsuchnamesareexemptfromtherelevantprotectivelawsandregulationsandthereforefreeforgeneraluse.
Thepublisher,theauthors,andtheeditorsaresafetoassumethattheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofpublication.
Neitherthepublishernortheauthorsortheeditorsgiveawarranty,expressorimplied,withrespecttothematerialcontainedhereinorforanyerrorsoromissionsthatmayhavebeenmade.
Thepublisherremainsneutralwithregardtojurisdictionalclaimsinpublishedmapsandinstitutionalafliations.
ThisHumanaPressimprintispublishedbytheregisteredcompanySpringerScience+BusinessMedia,LLC,partofSpringerNature.
Theregisteredcompanyaddressis:233SpringStreet,NewYork,NY10013,U.
S.
A.
DedicationThisbookisdedicatedtoGeorgeKlein,1925–2016,agiantcreativeandstimulatingscientistofinniteknowledge,friend,andmentor.
Hislegacyisanever-fadinglighthousetohundredsofformerstudents,co-workers,colleagues,andfriends.
vPrefaceAsecondeditionofSpringerProtocolsbookHumanMonoclonalAntibodiesisanaturaloutcomeoftherapiddevelopmentsintheareaandtheremarkableinterestattractedbyour2014rsteditionofthebook.
Itisamazingwhatarapidanddramaticdevelopmenthasoccurredintheeldofhumanmonoclonalantibodiessincethersttimethesewereproducedinthelaboratory.
ItwasthepioneeringstudyinitiatedbyProfessorGeorgeKleininStockholmin1977[1]thatshowedthefeasibilityofmakingsuchantibodiesfromimmortalizedperipheralbloodantigen-committedhumanBlymphocytes.
Theextensivebasicimmuneresearchwhichhastakenplaceduringthelastyearsrapidlyconvergedintotheclinic.
TheFastanddramaticdevelopmentofnovelimmunetreatmentsintheclinicusinghumanmonoclonalantibodiesurgedtheimprovementsoftraditionalandalsocompletelynewtechniquesinvolvedintheproductionoftheantibodies.
Theintroduc-tionofmonoclonalantibodiesdirectedagainstlymphocytecell-surfacecostimulatory/immunecheckpointreceptorsthatmediatetheimmuneresponsehasbeenrevolutionary.
Theseantibodiesenableovercomeimmuneunresponsiveness(i.
e.
,tolerance)ofTcells.
Antibodiesagainstcostimulatorylymphocytereceptorswhichnowareusedintheclinicprovedverybenecialfortumorpatientsatleastinrelationtosometypesofcancer.
Continuousprogressinmoleculartechniquesenablesgenuinemanagementofantibodymolecules.
SuchreagentsintroducedintocytotoxicTcellspromisenewhorizonsforthetreatmentofcancerpatients.
ThepresentSpringerProtocolsbookreectssomeoftherecentdevelopmentsinthearea.
Itincludesseveralcompletelynewchaptersrelatedtotopicsthatwerenotdiscussedintherstedition.
Inaddition,somechaptersfromthersteditionareupdatedwithnecessaryrevisions.
Similartotherstedition,besidesthedetailedspecictechnicalprotocols,thereareafewreviewmanuscriptstoo.
Jerusalem,IsraelMichaelSteinitzReference1.
SteinitzM,KleinG,KoskimiesS,Ma¨kela¨O(1977)EBVvirusinducedBlymphocytecelllinesproducingspecicantibody.
Nature269:420–422viiContentsPrefaceviiContributors.
xi1HumanMonoclonalAntibodies:TheBenetsofHumanization1HermanWaldmann2CancerImmunotherapy:TheDawnofAntibodyCocktails.
11IlariaMarrocco,DonatellaRomaniello,andYosefYarden3IgMNaturalAutoantibodiesinPhysiologyandtheTreatmentofDisease.
53MahboobehFereidan-Esfahani,TarekNayfeh,ArthurWarrington,CharlesL.
Howe,andMosesRodriguez4MonoclonalAntibodiesfortheTreatmentofMelanoma:PresentandFutureStrategies83MadhuriBhandaruandAnandRotte5AnEfcientMethodtoGenerateMonoclonalAntibodiesfromHumanBCells109JennaJ.
Guthmiller,HaleyL.
Dugan,KarlynnE.
Neu,LindaYu-LingLan,andPatrickC.
Wilson6IsolationofAntigen-Specic,Antibody-SecretingCellsUsingaChip-BasedImmunospotArray147HiroyukiKishi,TatsuhikoOzawa,HiroshiHamana,EijiKobayashi,andAtsushiMuraguchi7PuricationofHumanMonoclonalAntibodiesandTheirFragments.
163NicoleUlmer,SebastianVogg,ThomasMu¨ller-Spa¨th,andMassimoMorbidelli8One-TubeMulticolorFlowCytometryAssay(OTMA)forComprehensiveImmunophenotypingofPeripheralBlood189Anna-JasminaDonaubauer,PaulF.
Ru¨hle,InaBecker,RainerFietkau,UdoS.
Gaipl,andBenjaminFrey9HumanizationandSimultaneousOptimizationofMonoclonalAntibody213TaichiKuramochi,TomoyukiIgawa,HiroyukiTsunoda,andKunihiroHattori10AntibodyFragmentsHumanization:BeginningwiththeEndinMind231NicolasAubreyandPhilippeBilliald11Antigen-SpecicHumanMonoclonalAntibodiesfromTransgenicMice253SusanaMagadanMompoandAfricaGonzalez-Fernandez12ReningtheQualityofMonoclonalAntibodies:GraftingUniquePeptide-BindingSiteintheFabFramework293JeremyD.
KingandJohnC.
Williams13BasicProceduresforDetectionandCytotoxicityofChimericAntigenReceptors299KeichiroMihara,TetsumiYoshida,andJoyeetaBhattacharyyaix14RapidChimerizationofAntibodies307KojiHashimoto,KoheiKurosawa,HidetakaSeo,andKunihiroOhta15PhageDisplayTechnologyforHumanMonoclonalAntibodies.
319MarcoDalFerro,SerenaRizzo,EmanuelaRizzo,FrancescaMarano,ImmacolataLuisi,OlgaTarasiuk,andDanieleSblattero16RecombinantAntibodySelectionsbyCombiningPhageandYeastDisplay339FortunatoFerrara,MariaFeliciaSoluri,andDanieleSblattero17EpitopeMappingviaPhageDisplayfromSingle-GeneLibraries353ViolaFu¨hner,PhilipAlexanderHeine,KilianJohannesCarlZilkens,DorisMeier,KristianDanielRalphRoth,GustavoMarcalSchmidtGarciaMoreira,MichaelHust,andGiulioRusso18AHigh-ThroughputMagneticNanoparticle-BasedSemi-AutomatedAntibodyPhageDisplayBiopanning377AngelaChiewWenCh'ng,AzimahAhmad,ZoltanKonthur,andTheamSoonLim19ABindingPotencyAssayforPritumumabandEcto-DomainVimentin.
401IvanBabic,SantoshKesari,andMarkC.
Glassy20AMethodtoDetecttheBindingofHyper-GlycosylatedFragmentCrystallizable(Fc)RegionofHumanIgG1toGlycanReceptors417PatriciaBlundellandRichardPleass21ACell-BasedReporterAssayMeasuringtheActivationofFcGammaReceptorsInducedbyTherapeuticMonoclonalAntibodies423MichihikoAoyama,MinoruTada,andAkikoIshii-Watabe22"BIClonals":ProductionofBispecicAntibodiesinIgGFormatinTransientlyTransfectedMammalianCells.
431DanaLitvak-Greenfeld,LilachVaks,StavDror,LimorNahary,andItaiBenhar23ProductionofStabilizedAntibodyFragmentsintheE.
coliBacterialCytoplasmandinTransientlyTransfectedMammalianCells455RacheliBirnboim-Perach,YehuditGrinberg,LilachVaks,LimorNahary,andItaiBenharIndex481xContentsContributorsAZIMAHAHMADInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,MalaysiaMICHIHIKOAOYAMADivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanNICOLASAUBREYUMRUniversite-INRAISP1282,BioMAP,UniversitedeTours,Tours,FranceIVANBABICDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USAINABECKERDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyITAIBENHARSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelMADHURIBHANDARUDepartmentofDermatologyandSkinScience,UniversityofBritishColumbia,Vancouver,BC,CanadaJOYEETABHATTACHARYYADepartmentofCardiacResearch,CumballaHillHospitalandHeartInstitute,Mumbai,IndiaPHILIPPEBILLIALDSchoolofPharmacy,Paris-SudUniversity,Chatenay-Malabry,FranceRACHELIBIRNBOIM-PERACHSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelPATRICIABLUNDELLLiverpoolSchoolofTropicalMedicine,Liverpool,UKANGELACHIEWWENCH'NGInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,MalaysiaMARCODALFERRODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyANNA-JASMINADONAUBAUERDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanySTAVDRORSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelHALEYL.
DUGANCommitteeonImmunology,UniversityofChicago,Chicago,IL,USAMAHBOOBEHFEREIDAN-ESFAHANIDepartmentofNeurology,MayoClinic,Rochester,MN,USAFORTUNATOFERRARASpecicaInc.
,SantaFe,NM,USARAINERFIETKAUDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyBENJAMINFREYDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyVIOLAFU¨HNERAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyUDOS.
GAIPLDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyMARKC.
GLASSYDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USA;TranslationalNeuro-OncologyLaboratory,UCSDMooresCancerCenter,LaJolla,CA,USA;NascentBiotech,Inc.
,SanDiego,CA,USAxiAFRICAGONZALEZ-FERNANDEZImmunology,CentrodeInvestigacionesBiomedicas(CINBIO),CentrodeInvestigacionSingulardeGalicia,InstitutodeInvestigacionSanitariaGaliciaSur,UniversidaddeVigo,Vigo,SpainYEHUDITGRINBERGSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelJENNAJ.
GUTHMILLERDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USAHIROSHIHAMANADepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanKOJIHASHIMOTODepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanKUNIHIROHATTORIResearchDivision,ChugaiPharmaceutical,Kamakura,Kanagawa,JapanPHILIPALEXANDERHEINEAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyCHARLESL.
HOWEDepartmentofNeurology,MayoClinic,Rochester,MN,USAMICHAELHUSTAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyTOMOYUKIIGAWAChugaiPharmabodyResearchPte.
Ltd.
,Singapore,SingaporeAKIKOISHII-WATABEDivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanSANTOSHKESARIDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USAJEREMYD.
KINGDepartmentofMolecularMedicine,BeckmanResearchInstituteatCityofHope,Duarte,CA,USAHIROYUKIKISHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanEIJIKOBAYASHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanZOLTANKONTHURDepartmentofBiomolecularSystems,MaxPlanckInstituteofColloidsandInterfaces,Potsdam,GermanyTAICHIKURAMOCHIChugaiPharmabodyResearchPte.
Ltd.
,Singapore,SingaporeKOHEIKUROSAWADepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanLINDAYU-LINGLANCommitteeonImmunology,UniversityofChicago,Chicago,IL,USATHEAMSOONLIMInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,Malaysia;AnalyticalBiochemistryResearchCentre,UniversitiSainsMalaysia,Penang,MalaysiaDANALITVAK-GREENFELDSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelIMMACOLATALUISIDepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalySUSANAMAGADANMOMPOImmunology,CentrodeInvestigacionesBiomedicas(CINBIO),CentrodeInvestigacionSingulardeGalicia,InstitutodeInvestigacionSanitariaGaliciaSur,UniversidaddeVigo,Vigo,SpainFRANCESCAMARANODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyILARIAMARROCCODepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelxiiContributorsDORISMEIERAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyKEICHIROMIHARADepartmentofHematologyandOncology,ResearchInstituteforRadiationBiologyandMedicine,HiroshimaUniversity,Hiroshima,JapanMASSIMOMORBIDELLIETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandGUSTAVOMARCALSCHMIDTGARCIAMOREIRAAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyTHOMASMU¨LLER-SPA¨THETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandATSUSHIMURAGUCHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanLIMORNAHARYSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelTAREKNAYFEHDepartmentofNeurology,MayoClinic,Rochester,MN,USAKARLYNNE.
NEUDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USA;CommitteeonImmunology,UniversityofChicago,Chicago,IL,USAKUNIHIROOHTADepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanTATSUHIKOOZAWADepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanRICHARDPLEASSLiverpoolSchoolofTropicalMedicine,Liverpool,UKSERENARIZZODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyEMANUELARIZZODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyMOSESRODRIGUEZDepartmentofNeurology,MayoClinic,Rochester,MN,USADONATELLAROMANIELLODepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelKRISTIANDANIELRALPHROTHAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyANANDROTTEDepartmentofClinicalPharmacology,GenentechResearchandEarlyDevelopment,SouthSanFrancisco,CA,USA;DepartmentofClinicalandRegulatoryAffairs,NevroCorp.
,RedwoodCity,CA,USAPAULF.
RU¨HLEDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyGIULIORUSSOAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyDANIELESBLATTERODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyHIDETAKASEODepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanMARIAFELICIASOLURIDepartmentofHealthSciencesandIRCAD,UniversityofPiemonteOrientale(UPO),Novara,ItalyMINORUTADADivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanOLGATARASIUKDepartmentofHealthSciencesandIRCAD,UniversityofEasternPiedmont,Novara,ItalyContributorsxiiiHIROYUKITSUNODAResearchDivision,ChugaiPharmaceutical,Kamakura,Kanagawa,JapanNICOLEULMERETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandLILACHVAKSSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelSEBASTIANVOGGETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandHERMANWALDMANNSirWilliamDunnSchoolofPathology,OxfordUniversity,Oxford,UKARTHURWARRINGTONDepartmentofNeurology,MayoClinic,Rochester,MN,USAJOHNC.
WILLIAMSDepartmentofMolecularMedicine,BeckmanResearchInstituteatCityofHope,Duarte,CA,USAPATRICKC.
WILSONDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USA;CommitteeonImmunology,UniversityofChicago,Chicago,IL,USAYOSEFYARDENDepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelTETSUMIYOSHIDADepartmentofHematologyandOncology,ResearchInstituteforRadiationBiologyandMedicine,HiroshimaUniversity,Hiroshima,JapanKILIANJOHANNESCARLZILKENSYUMABGmbH,Braunschweig,GermanyxivContributors
WalkerSchoolofLifeandMedicalSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForfurthervolumes:http://www.
springer.
com/series/7651HumanMonoclonalAntibodiesMethodsandProtocolsSecondEditionEditedbyMichaelSteinitzDepartmentofPathology,TheHebrewUniversity,HadassahMedicalSchool,Jerusalem,IsraelEditorMichaelSteinitzDepartmentofPathologyTheHebrewUniversityHadassahMedicalSchoolJerusalem,IsraelISSN1064-3745ISSN1940-6029(electronic)MethodsinMolecularBiologyISBN978-1-4939-8957-7ISBN978-1-4939-8958-4(eBook)https://doi.
org/10.
1007/978-1-4939-8958-4LibraryofCongressControlNumber:2018964050SpringerScience+BusinessMedia,LLC,partofSpringerNature2019Thisworkissubjecttocopyright.
AllrightsarereservedbythePublisher,whetherthewholeorpartofthematerialisconcerned,specicallytherightsoftranslation,reprinting,reuseofillustrations,recitation,broadcasting,reproductiononmicrolmsorinanyotherphysicalway,andtransmissionorinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdeveloped.
Theuseofgeneraldescriptivenames,registerednames,trademarks,servicemarks,etc.
inthispublicationdoesnotimply,evenintheabsenceofaspecicstatement,thatsuchnamesareexemptfromtherelevantprotectivelawsandregulationsandthereforefreeforgeneraluse.
Thepublisher,theauthors,andtheeditorsaresafetoassumethattheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofpublication.
Neitherthepublishernortheauthorsortheeditorsgiveawarranty,expressorimplied,withrespecttothematerialcontainedhereinorforanyerrorsoromissionsthatmayhavebeenmade.
Thepublisherremainsneutralwithregardtojurisdictionalclaimsinpublishedmapsandinstitutionalafliations.
ThisHumanaPressimprintispublishedbytheregisteredcompanySpringerScience+BusinessMedia,LLC,partofSpringerNature.
Theregisteredcompanyaddressis:233SpringStreet,NewYork,NY10013,U.
S.
A.
DedicationThisbookisdedicatedtoGeorgeKlein,1925–2016,agiantcreativeandstimulatingscientistofinniteknowledge,friend,andmentor.
Hislegacyisanever-fadinglighthousetohundredsofformerstudents,co-workers,colleagues,andfriends.
vPrefaceAsecondeditionofSpringerProtocolsbookHumanMonoclonalAntibodiesisanaturaloutcomeoftherapiddevelopmentsintheareaandtheremarkableinterestattractedbyour2014rsteditionofthebook.
Itisamazingwhatarapidanddramaticdevelopmenthasoccurredintheeldofhumanmonoclonalantibodiessincethersttimethesewereproducedinthelaboratory.
ItwasthepioneeringstudyinitiatedbyProfessorGeorgeKleininStockholmin1977[1]thatshowedthefeasibilityofmakingsuchantibodiesfromimmortalizedperipheralbloodantigen-committedhumanBlymphocytes.
Theextensivebasicimmuneresearchwhichhastakenplaceduringthelastyearsrapidlyconvergedintotheclinic.
TheFastanddramaticdevelopmentofnovelimmunetreatmentsintheclinicusinghumanmonoclonalantibodiesurgedtheimprovementsoftraditionalandalsocompletelynewtechniquesinvolvedintheproductionoftheantibodies.
Theintroduc-tionofmonoclonalantibodiesdirectedagainstlymphocytecell-surfacecostimulatory/immunecheckpointreceptorsthatmediatetheimmuneresponsehasbeenrevolutionary.
Theseantibodiesenableovercomeimmuneunresponsiveness(i.
e.
,tolerance)ofTcells.
Antibodiesagainstcostimulatorylymphocytereceptorswhichnowareusedintheclinicprovedverybenecialfortumorpatientsatleastinrelationtosometypesofcancer.
Continuousprogressinmoleculartechniquesenablesgenuinemanagementofantibodymolecules.
SuchreagentsintroducedintocytotoxicTcellspromisenewhorizonsforthetreatmentofcancerpatients.
ThepresentSpringerProtocolsbookreectssomeoftherecentdevelopmentsinthearea.
Itincludesseveralcompletelynewchaptersrelatedtotopicsthatwerenotdiscussedintherstedition.
Inaddition,somechaptersfromthersteditionareupdatedwithnecessaryrevisions.
Similartotherstedition,besidesthedetailedspecictechnicalprotocols,thereareafewreviewmanuscriptstoo.
Jerusalem,IsraelMichaelSteinitzReference1.
SteinitzM,KleinG,KoskimiesS,Ma¨kela¨O(1977)EBVvirusinducedBlymphocytecelllinesproducingspecicantibody.
Nature269:420–422viiContentsPrefaceviiContributors.
xi1HumanMonoclonalAntibodies:TheBenetsofHumanization1HermanWaldmann2CancerImmunotherapy:TheDawnofAntibodyCocktails.
11IlariaMarrocco,DonatellaRomaniello,andYosefYarden3IgMNaturalAutoantibodiesinPhysiologyandtheTreatmentofDisease.
53MahboobehFereidan-Esfahani,TarekNayfeh,ArthurWarrington,CharlesL.
Howe,andMosesRodriguez4MonoclonalAntibodiesfortheTreatmentofMelanoma:PresentandFutureStrategies83MadhuriBhandaruandAnandRotte5AnEfcientMethodtoGenerateMonoclonalAntibodiesfromHumanBCells109JennaJ.
Guthmiller,HaleyL.
Dugan,KarlynnE.
Neu,LindaYu-LingLan,andPatrickC.
Wilson6IsolationofAntigen-Specic,Antibody-SecretingCellsUsingaChip-BasedImmunospotArray147HiroyukiKishi,TatsuhikoOzawa,HiroshiHamana,EijiKobayashi,andAtsushiMuraguchi7PuricationofHumanMonoclonalAntibodiesandTheirFragments.
163NicoleUlmer,SebastianVogg,ThomasMu¨ller-Spa¨th,andMassimoMorbidelli8One-TubeMulticolorFlowCytometryAssay(OTMA)forComprehensiveImmunophenotypingofPeripheralBlood189Anna-JasminaDonaubauer,PaulF.
Ru¨hle,InaBecker,RainerFietkau,UdoS.
Gaipl,andBenjaminFrey9HumanizationandSimultaneousOptimizationofMonoclonalAntibody213TaichiKuramochi,TomoyukiIgawa,HiroyukiTsunoda,andKunihiroHattori10AntibodyFragmentsHumanization:BeginningwiththeEndinMind231NicolasAubreyandPhilippeBilliald11Antigen-SpecicHumanMonoclonalAntibodiesfromTransgenicMice253SusanaMagadanMompoandAfricaGonzalez-Fernandez12ReningtheQualityofMonoclonalAntibodies:GraftingUniquePeptide-BindingSiteintheFabFramework293JeremyD.
KingandJohnC.
Williams13BasicProceduresforDetectionandCytotoxicityofChimericAntigenReceptors299KeichiroMihara,TetsumiYoshida,andJoyeetaBhattacharyyaix14RapidChimerizationofAntibodies307KojiHashimoto,KoheiKurosawa,HidetakaSeo,andKunihiroOhta15PhageDisplayTechnologyforHumanMonoclonalAntibodies.
319MarcoDalFerro,SerenaRizzo,EmanuelaRizzo,FrancescaMarano,ImmacolataLuisi,OlgaTarasiuk,andDanieleSblattero16RecombinantAntibodySelectionsbyCombiningPhageandYeastDisplay339FortunatoFerrara,MariaFeliciaSoluri,andDanieleSblattero17EpitopeMappingviaPhageDisplayfromSingle-GeneLibraries353ViolaFu¨hner,PhilipAlexanderHeine,KilianJohannesCarlZilkens,DorisMeier,KristianDanielRalphRoth,GustavoMarcalSchmidtGarciaMoreira,MichaelHust,andGiulioRusso18AHigh-ThroughputMagneticNanoparticle-BasedSemi-AutomatedAntibodyPhageDisplayBiopanning377AngelaChiewWenCh'ng,AzimahAhmad,ZoltanKonthur,andTheamSoonLim19ABindingPotencyAssayforPritumumabandEcto-DomainVimentin.
401IvanBabic,SantoshKesari,andMarkC.
Glassy20AMethodtoDetecttheBindingofHyper-GlycosylatedFragmentCrystallizable(Fc)RegionofHumanIgG1toGlycanReceptors417PatriciaBlundellandRichardPleass21ACell-BasedReporterAssayMeasuringtheActivationofFcGammaReceptorsInducedbyTherapeuticMonoclonalAntibodies423MichihikoAoyama,MinoruTada,andAkikoIshii-Watabe22"BIClonals":ProductionofBispecicAntibodiesinIgGFormatinTransientlyTransfectedMammalianCells.
431DanaLitvak-Greenfeld,LilachVaks,StavDror,LimorNahary,andItaiBenhar23ProductionofStabilizedAntibodyFragmentsintheE.
coliBacterialCytoplasmandinTransientlyTransfectedMammalianCells455RacheliBirnboim-Perach,YehuditGrinberg,LilachVaks,LimorNahary,andItaiBenharIndex481xContentsContributorsAZIMAHAHMADInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,MalaysiaMICHIHIKOAOYAMADivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanNICOLASAUBREYUMRUniversite-INRAISP1282,BioMAP,UniversitedeTours,Tours,FranceIVANBABICDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USAINABECKERDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyITAIBENHARSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelMADHURIBHANDARUDepartmentofDermatologyandSkinScience,UniversityofBritishColumbia,Vancouver,BC,CanadaJOYEETABHATTACHARYYADepartmentofCardiacResearch,CumballaHillHospitalandHeartInstitute,Mumbai,IndiaPHILIPPEBILLIALDSchoolofPharmacy,Paris-SudUniversity,Chatenay-Malabry,FranceRACHELIBIRNBOIM-PERACHSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelPATRICIABLUNDELLLiverpoolSchoolofTropicalMedicine,Liverpool,UKANGELACHIEWWENCH'NGInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,MalaysiaMARCODALFERRODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyANNA-JASMINADONAUBAUERDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanySTAVDRORSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelHALEYL.
DUGANCommitteeonImmunology,UniversityofChicago,Chicago,IL,USAMAHBOOBEHFEREIDAN-ESFAHANIDepartmentofNeurology,MayoClinic,Rochester,MN,USAFORTUNATOFERRARASpecicaInc.
,SantaFe,NM,USARAINERFIETKAUDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyBENJAMINFREYDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyVIOLAFU¨HNERAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyUDOS.
GAIPLDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyMARKC.
GLASSYDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USA;TranslationalNeuro-OncologyLaboratory,UCSDMooresCancerCenter,LaJolla,CA,USA;NascentBiotech,Inc.
,SanDiego,CA,USAxiAFRICAGONZALEZ-FERNANDEZImmunology,CentrodeInvestigacionesBiomedicas(CINBIO),CentrodeInvestigacionSingulardeGalicia,InstitutodeInvestigacionSanitariaGaliciaSur,UniversidaddeVigo,Vigo,SpainYEHUDITGRINBERGSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelJENNAJ.
GUTHMILLERDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USAHIROSHIHAMANADepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanKOJIHASHIMOTODepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanKUNIHIROHATTORIResearchDivision,ChugaiPharmaceutical,Kamakura,Kanagawa,JapanPHILIPALEXANDERHEINEAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyCHARLESL.
HOWEDepartmentofNeurology,MayoClinic,Rochester,MN,USAMICHAELHUSTAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyTOMOYUKIIGAWAChugaiPharmabodyResearchPte.
Ltd.
,Singapore,SingaporeAKIKOISHII-WATABEDivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanSANTOSHKESARIDepartmentofTranslationalNeurosciencesandNeurotherapeutics,JohnWayneCancerInstitute,SantaMonica,CA,USAJEREMYD.
KINGDepartmentofMolecularMedicine,BeckmanResearchInstituteatCityofHope,Duarte,CA,USAHIROYUKIKISHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanEIJIKOBAYASHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanZOLTANKONTHURDepartmentofBiomolecularSystems,MaxPlanckInstituteofColloidsandInterfaces,Potsdam,GermanyTAICHIKURAMOCHIChugaiPharmabodyResearchPte.
Ltd.
,Singapore,SingaporeKOHEIKUROSAWADepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanLINDAYU-LINGLANCommitteeonImmunology,UniversityofChicago,Chicago,IL,USATHEAMSOONLIMInstituteforResearchinMolecularMedicine,UniversitiSainsMalaysia,Penang,Malaysia;AnalyticalBiochemistryResearchCentre,UniversitiSainsMalaysia,Penang,MalaysiaDANALITVAK-GREENFELDSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelIMMACOLATALUISIDepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalySUSANAMAGADANMOMPOImmunology,CentrodeInvestigacionesBiomedicas(CINBIO),CentrodeInvestigacionSingulardeGalicia,InstitutodeInvestigacionSanitariaGaliciaSur,UniversidaddeVigo,Vigo,SpainFRANCESCAMARANODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyILARIAMARROCCODepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelxiiContributorsDORISMEIERAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyKEICHIROMIHARADepartmentofHematologyandOncology,ResearchInstituteforRadiationBiologyandMedicine,HiroshimaUniversity,Hiroshima,JapanMASSIMOMORBIDELLIETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandGUSTAVOMARCALSCHMIDTGARCIAMOREIRAAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyTHOMASMU¨LLER-SPA¨THETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandATSUSHIMURAGUCHIDepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanLIMORNAHARYSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelTAREKNAYFEHDepartmentofNeurology,MayoClinic,Rochester,MN,USAKARLYNNE.
NEUDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USA;CommitteeonImmunology,UniversityofChicago,Chicago,IL,USAKUNIHIROOHTADepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanTATSUHIKOOZAWADepartmentofImmunology,GraduateSchoolofMedicineandPharmaceuticalSciences,UniversityofToyama,Toyama,JapanRICHARDPLEASSLiverpoolSchoolofTropicalMedicine,Liverpool,UKSERENARIZZODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyEMANUELARIZZODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyMOSESRODRIGUEZDepartmentofNeurology,MayoClinic,Rochester,MN,USADONATELLAROMANIELLODepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelKRISTIANDANIELRALPHROTHAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyANANDROTTEDepartmentofClinicalPharmacology,GenentechResearchandEarlyDevelopment,SouthSanFrancisco,CA,USA;DepartmentofClinicalandRegulatoryAffairs,NevroCorp.
,RedwoodCity,CA,USAPAULF.
RU¨HLEDepartmentofRadiationOncology,Universita¨tsklinikumErlangen,Friedrich-Alexander-Universita¨tErlangen-Nu¨rnberg,Erlangen,GermanyGIULIORUSSOAbteilungBiotechnologie,Institutfu¨rBiochemie,BiotechnologieundBioinformatik,TechnischeUniversita¨tBraunschweig,Braunschweig,GermanyDANIELESBLATTERODepartmentofLifeSciences,UniversityofTrieste,Trieste,ItalyHIDETAKASEODepartmentofLifeSciences,GraduateSchoolofArtsandSciences,TheUniversityofTokyo,Tokyo,JapanMARIAFELICIASOLURIDepartmentofHealthSciencesandIRCAD,UniversityofPiemonteOrientale(UPO),Novara,ItalyMINORUTADADivisionofBiologicalChemistryandBiologicals,NationalInstituteofHealthSciences,Kawasaki,Kanagawa,JapanOLGATARASIUKDepartmentofHealthSciencesandIRCAD,UniversityofEasternPiedmont,Novara,ItalyContributorsxiiiHIROYUKITSUNODAResearchDivision,ChugaiPharmaceutical,Kamakura,Kanagawa,JapanNICOLEULMERETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandLILACHVAKSSchoolofMolecularCellBiologyandBiotechnology,TheGeorgeS.
WiseFacultyofLifeSciences,Tel-AvivUniversity,RamatAviv,IsraelSEBASTIANVOGGETHZurich,InstituteforChemicalandBioengineering,Zurich,SwitzerlandHERMANWALDMANNSirWilliamDunnSchoolofPathology,OxfordUniversity,Oxford,UKARTHURWARRINGTONDepartmentofNeurology,MayoClinic,Rochester,MN,USAJOHNC.
WILLIAMSDepartmentofMolecularMedicine,BeckmanResearchInstituteatCityofHope,Duarte,CA,USAPATRICKC.
WILSONDepartmentofMedicine,SectionofRheumatology,TheKnappCenterforLupusandImmunology,UniversityofChicago,Chicago,IL,USA;CommitteeonImmunology,UniversityofChicago,Chicago,IL,USAYOSEFYARDENDepartmentofBiologicalRegulation,WeizmannInstituteofScience,Rehovot,IsraelTETSUMIYOSHIDADepartmentofHematologyandOncology,ResearchInstituteforRadiationBiologyandMedicine,HiroshimaUniversity,Hiroshima,JapanKILIANJOHANNESCARLZILKENSYUMABGmbH,Braunschweig,GermanyxivContributors
- Efcientwww.idanmu.com相关文档
- zahraniwww.idanmu.com
- (1717MU)木瓜奇迹里的战士,木瓜奇迹mu官网
- lenkovchwww.idanmu.com
- Similarlywww.idanmu.com
- kultuewww.idanmu.com
- Alimanwww.idanmu.com
RAKsmart 2021新年新增韩国服务器及香港美国日本VPS半价
RAKsmart 商家我们肯定不算陌生，目前主要的营销客户群肯定是我们。于是在去年的时候有新增很多很多的机房，比如也有测试过的日本、香港、美国机房，这不今年有新增韩国机房（记得去年是不是也有增加过）。且如果没有记错的话，之前VPS主机也有一次磁盘故障的问题。 这不今天有看到商家新增韩国服务器产品，当然目前我还不清楚商家韩国服务器的线路和速度情况，后面我搞一台测试机进行...
PIGYun中秋特惠：香港/韩国VPS月付14元起
PIGYun发布了九月份及中秋节特惠活动，提供8折优惠码，本月商家主推中国香港和韩国机房，优惠后最低韩国每月14元/中国香港每月19元起。这是一家成立于2019年的国人商家，提供中国香港、韩国和美国等地区机房VPS主机，基于KVM架构，采用SSD硬盘，CN2+BGP线路(美国为CUVIP-AS9929、GIA等)。下面列出两款主机配置信息。机房：中国香港CPU：1core内存：1GB硬盘：10GB...
HostKvm：夏季优惠,香港云地/韩国vps终身7折,线路好/机器稳/适合做站
hostkvm怎么样?hostkvm是一家国内老牌主机商家，商家主要销售KVM架构的VPS，目前有美国、日本、韩国、中国香港等地的服务，站长目前还持有他家香港CN2线路的套餐，已经用了一年多了，除了前段时间香港被整段攻击以外，一直非常稳定，是做站的不二选择，目前商家针对香港云地和韩国机房的套餐进行7折优惠，其他套餐为8折，商家支持paypal和支付宝付款。点击进入：hostkvm官方网站地址hos...
www.idanmu.com为你推荐
-
固态硬盘是什么什么是固态硬盘?敬汉卿姓名被抢注身份证号码被别人抢注了12306帐号怎么办摩根币摩根币是怎么骗人的？甲骨文不满赔偿如果合同期不满被单位辞退，用人单位是否需要赔偿sss17.com一玩棋牌吧（www.17wqp.com）怎么样？789se.comwuwu8.com这个站长是谁？qq530.com求教：如何下载http://www.qq530.com/ 上的音乐www.78222.com我看一个网站.www.snw58.com里面好有意思呀，不知道里面的信息是不是真实的斗城网女追男有多易？喜欢你，可我不知道你喜不喜欢我！！平安夜希望有他陪我过yinrentangzimotang氨基酸洗发水的功效咋样？