Rehydratewww

www.haole012.com  时间:2021-03-01  阅读:()
www.
ptglab.
comPRODUCT-SPECIFICPROTOCOLSIHC(10782-2-AP)SampletypeSamplepreparationAntigenRetrievalBlockingbufferPrimaryantibodydilutionIncubationtimeSignaldetectionmousebraintissueParaffin-embeddedTris-EDTAbuffer(pH9)5%goatseruminTBS1:20001.
5hatroomtempDako/AgilentEnvisionkitmousebraintissueParaffin-embeddedNone5%goatseruminTBS1:2001.
5hatroomtempDako/AgilentEnvisionkitmousebraintissueParaffin-embeddedNone5%goatseruminTBS1:2001.
5hatroomtempDako/AgilentEnvisionkitratbraintissueParaffin-embeddedNone5%goatseruminTBS1:2001.
5hatroomtempDako/AgilentEnvisionkitPROTOCOL(moredetailsavailableuponrequest):1.
Deparaffinizesectionsin2bathsofxylenefor10mineach.
2.
Rehydratesectionsbysequentialincubationwith100%,95%,80%,and60%ethanol,5minforeachbath.
3.
Rinsesectionswithdistilledwater3timesfor3mineach.
AntigenRetrieval(checktableabove)4.
Transfersectionstoacontainerandcoverwithantigenretrievalsolutionaccordingtothetableabove.
5.
Heatslidesinamicrowaveonmediumpowerfor10min.
6.
Allowslidestocoolinthebufferfor35min.
IncubationwithPrimaryAntibodyandSignalDetection7.
Rinseslides3timeswith1XTBSfor3mineach.
8.
Incubateslideswith3%H2O2solution(dilutedindistilledwater)for10mintoquenchendogenousperoxidaseactivity.
9.
Rinseslides3timeswith1XTBSfor3mineach.
10.
Blockthesectionsatroomtemperaturefor1hinBlockingbuffer.
11.
Incubatesectionswithprimaryantibodyin1XTBS12.
Rinseslides3timeswith1XTBSfor3mineach.
13.
Performsignaldetectionprotocolaccordingtothemanufacturer'sinstructions.
14.
Immerseslidesinabathofhematoxylintostainthenucleifor3min.
15.
Rinseslidesgentlyinadistilledwaterbath.
16.
Transferslidestoasolutioncontaining1%HCland99%ethanolfor10sec,andthenintodistilledwaterimmediately.
17.
Immerseslidessequentiallyinto60%,80%,90%,and100%ethanolbathfor5mineach.
18.
Incubateslidesinxylenebathfor5min,remove,thenincubateagainfor5min.
19.
Mountthesectionwithasmalldropofneutralbalsamandaddacoverslip.
Air-dryslidesinthehood.
20.
Examineslidesunderamicroscope.
Visitptglab.
comforfullproductrange

ReliableSite:美国服务器租用,洛杉矶/纽约/迈阿密等机房;E3-1240V6/64GB/1TSSD,$95/月

reliablesite怎么样?reliablesite是一家于2006年成立的老牌美国主机商,主要提供独服,数据中心有迈阿密、纽约、洛杉矶等,均免费提供20Gbps DDoS防护,150TB月流量,1Gbps带宽。月付19美金可升级为10Gbps带宽。洛杉矶/纽约/迈阿密等机房,E3-1240V6/64GB内存/1TB SSD硬盘/DDOS/150TB流量/1Gbps带宽/DDOS,$95/月,...

蓝竹云挂机宝25元/年,美国西雅图 1核1G 100M 20元

蓝竹云怎么样 蓝竹云好不好蓝竹云是新商家这次给我们带来的 挂机宝25元/年 美国西雅图云服务器 下面是套餐和评测,废话不说直接开干~~蓝竹云官网链接点击打开官网江西上饶挂机宝宿主机配置 2*E5 2696V2 384G 8*1500G SAS RAID10阵列支持Windows sever 2008,Windows sever 2012,Centos 7.6,Debian 10.3,Ubuntu1...

Hostodo独立日提供四款特价年付VPS套餐 最低年付$13.99

前天,还有在"Hostodo商家提供两款大流量美国VPS主机 可选拉斯维加斯和迈阿密"文章中提到有提供两款流量较大的套餐,这里今天看到有发布四款庆祝独立日的七月份的活动,最低年付VPS主机13.99美元,如果有需要年付便宜VPS主机的可以选择商家。目前,Hostodo机房可选拉斯维加斯和迈阿密两个数据中心,且都是基于KVM虚拟+NVMe整列,年付送DirectAdmin授权,需要发工单申请。(如何...

www.haole012.com为你推荐
今日油条油条是怎样由来咏春大师被ko大师:咏春是不会败的 教练:能不偷袭吗,咏春拳教练广东GDP破10万亿广东省2019年各市gdp是多少?22zizi.comwww 地址 didi22怎么打不开了,还有好看的吗>com同ip网站一个域名能对应多个IP吗www.sesehu.comwww.121gao.com 是谁的网站啊www.javmoo.comJAV编程怎么做?杨丽晓博客明星的最新博文广告法请问违反了广告法,罚款的标准是什么dpscycleDPScycle插件为什么没有猎人模块 最好详细点
vps论坛 华为云服务 瓦工 国外私服 42u机柜尺寸 网络星期一 贵州电信宽带测速 圣诞节促销 qq数据库 免费mysql howfile 环聊 秒杀品 免费个人主页 免费网络空间 移动王卡 脚本大全 ncp restart 更多