melpomenejufy

SupplementSupplementalFigure1.
Taxonomicinstabilityindicesbasedon100maximumlikelihoodtreeinferences.
Thedashedlineshowsthechosencutoff,anindexof5000.
Mosttaxa,92%,fallbelowthiswhileabovethisvalueincreasesquickly.
SupplementalFigure2.
Microsatelliteinferenceandthetypeofsequencing(short,Sanger,long)usingvertebrategenomedataset.
Thisshowstherelationshipbetweeninferenceof2-mer,3-mer,4-mer,5-mer,6-mer,andtotal(2-6mer)microsatellitecountandthetypesofsequencingwithphylogeneticANOVAvaluesintheupperlefthandforeachtype.
050100150200200030004000500060007000TaxaTaxanomicInstabilityIndex2mercount01000020000300004000050000PValue=0.
9773mercount0100020003000400050006000PValue=0.
9534mercount02000400060008000PValue=0.
285mercountTypeofSequencing050010001500200025003000LongSangerShortPValue=0.
0886mercountTypeofSequencing050010001500LongSangerShortPValue=0.
62626mercountTypeofSequencing0100002000030000400005000060000LongSangerShortPValue=0.
902SupplementalFigure3.
Measuresofthequalityofgenomes(scaffoldN50,contigN50,andgenomesize)andmicrosatelliteinference.
Linearmodelp-valuesareindicatedintheupperright-handcornerforeachoftheanalyses.
12345050010001500GenomeSizeinGbp26mer(bp/mb)pvalue=0.
2410e+002e+074e+07050010001500ContigN5026mer(bp/mb)pvalue=0.
7050.
0e+004.
0e+078.
0e+071.
2e+08050010001500ScaffoldN5026mer(bp/mb)pvalue=0.
177SupplementalFigure4.
BUSCOscoresandmicrosatellitecontent(bp/Mbp).
Theverticalblacklineindicatesthethresholdof90thatwasrequiredforinclusioninourstudy.
Therewere83genomesthatwerediscardedbasedonthisthreshold.
0.
00.
20.
40.
60.
81.
00e+002e+074e+07BUSCOScoreMicrosatContent(bp)SupplementalFigure5.
Tipratesformicrosatelliteevolutionacrosseachofthetaxainourphylogeny.
Thebarsattheendofeachtiprepresentstheabsolutevalueofthemicrosatelliteevolutiontipratesforeachtaxa.
BlattodeaHemipteraHymenopteraColeopteraDipteraLepidopteraOdonataEphemeropteraSupplementalTable1.
Allgenomesandaccessionnumbersforthe303insectspeciesthatareincludedintheanalyses.
GenomeassembliesweredownloadedfromNCBI(https://www.
ncbi.
nlm.
nih.
gov),ENSEMBL(https://useast.
ensembl.
org/index.
html),andBaylorHGC(https://www.
hgsc.
bcm.
edu/arthropods/i5k).
OrderSpeciesAccessionNotesLepidopteraAchalaruslyciadesMOOZ01000001.
1HymenopteraAcromyrmexechinatiorNW_011623521.
1HemipteraAcyrthosiphonpisumGL349630DipteraAedesaegyptiGCF_002204515.
2_AaegL5.
0DipteraAedesalbopictusNW_017855997.
1ColeopteraAethinatumidaNW_017852934ColeopteraAgrilusplanipennisNW_017259594ColeopteraAleocharabilineataNBZA01000001PlecopteraAmphinemurasulcicollisLVVV01000001LepidopteraAmyeloistransitellaNW_013534156DipteraAnophelesalnimanusCM008152DipteraAnophelesaquasalisNJHH01000001DipteraAnophelesarabiensisKB704125DipteraAnophelesatroparvusKI421882DipteraAnopheleschristyiKB673756DipteraAnophelescoluzziiEQ097730DipteraAnophelescracensKZ067108DipteraAnophelesculicifaciesKI422464DipteraAnophelesdarlingiADMH00000000DipteraAnophelesdirusKB672490DipteraAnophelesepiroticusKB669613DipteraAnophelesfarautiKI915040DipteraAnophelesfunestusKB668221OrderSpeciesAccessionNotesDipteraAnophelesgambiaeAAAB00000000DipteraAnopheleskoliensisJXXB01000001DipteraAnophelesmaculatusKZ061539DipteraAnophelesmelasKI919284DipteraAnophelesmerusKI915156DipteraAnophelesminimusKB663610DipteraAnophelesniliATLZ01000001DipteraAnophelespunctulatusJXXA01000001DipteraAnophelesquadriannulatusKB665398DipteraAnophelessinensisKL639024DipteraAnophelesstephensiKE388890ColeopteraAnoplophoraglabripennisKB933500LepidopteraAntheraeayamamaiPRJNA383008GigaDBDatabaseHymenopteraAphaenogasterashmeadiNJRQ01000001HymenopteraAphaenogasterfloridanaNJRP01000001HymenopteraAphaenogasterfulvaNJRO01000001HymenopteraAphaenogastermiamianaNJRN01000001HymenopteraAphaenogasterpiceaNJRM01000001HymenopteraAphaenogasterrudisNJRL01000001HymenopteraApisceranaNW_016017455HymenopteraApisdorsataNW_006263304HymenopteraApisfloreaNW_003789104HymenopteraApismelliferaNC_037638-NC_0376531dna:chromosome_groupchromosome_group:Amel_4.
5:1:1:29893408:1REFHymenopteraAthaliarosaeNW_012161901HymenopteraAttacephalotesGCF_000143395>scaffold00001dna:supercontigsupercontig:Attacep1.
0:scaffold00001:1:15679321:1REFHymenopteraAttacolombicaNW_017258044OrderSpeciesAccessionNotesDipteraBactroceradorsalisNW_011869236DipteraBactroceralatifronsNW_017534406DipteraBactroceraoleaeNW_013581209DipteraBactroceratryoniJHQJ01000001EphemeropteraBaetisrhodaniLVVX01000001DipteraBelgicaantarcticaJPYR01000001HemipteraBemisiatabaciNW_017547088LepidopteraBicyclusanynanaNW_019862748BlattodeaBlattellagermanicaPYGN01000001HymenopteraBombusimpatiensJH157811HymenopteraBombusterrestrisCM001169-CM001186>B01dna:chromosome_groupchromosome_group:Bter_1.
0:B01:1:17153651:1REFLepidopteraBombyxhuttoniASCI01000001LepidopteraBombyxmoriGCF_000151625>scaf1dna:supercontigsupercontig:ASM15162v1:scaf1:1:16203812:1REFLepidopteraCalephelisnemesisNJDD01000001LepidopteraCalephelisvirginiensisNJDC01000001DipteraCalliphoravicinaJXOT01000001OdonataCalopteryxsplendensLYUA01000001LepidopteraCalycopiscecropsLUGF01000001HymenopteraCamponotusfloridanusNW_020229214DipluraCatajapyxaquilonarisKZ537955HymenopteraCecidostibafungosaUCOJ01000001HymenopteraCecidostibasemifasciaUCQR01000001IsopteraCEME01microsat.
txtCEME01000001HymenopteraCephuscinctusNW_014332639HymenopteraCeratinacalcarataNW_017130860DipteraCeratitiscapitataNW_019376232HymenopteraCeratosolensolmsiNW_011948412OrderSpeciesAccessionNotesDipteraChaoborustrivitattusJXOU01000001LepidopteraChilosuppressalisANCD01000001DipteraChironomusripariusJXPV01000001DipteraChironomustentansHG428765HemipteraCimexhemipterusLRFB01000001HemipteraCimexlectulariusNW_019942502DipteraCirrulahiansJXOS01000001PhasmatodeaClitarchushookeriNQII01000001DipteraClogmiaalbipunctataJXOV01000001DipteraCluniomarinusCVRI01000001DipteraCoboldiafuscipesJXOR01001678DipteraCondylostyluspatibulatusJXOW01000001HymenopteraCopidosomafloridanumNW_019379452HymenopteraCotesiavestalisJZSA01000001BlattodeaCryptotermessecundusNW_019718022DipteraCulexquinquefasciatusNZ_AAWU00000000.
1>supercont3.
1dna:supercontigsupercontig:CpipJ2:supercont3.
1:1:3873040:1REFDipteraCulicoidessonorensisOGVF02000001HymenopteraCyphomyrmexcostatusNW_017265214HemipteraDactylopiuscoccusJMCM01001127LepidopteraDanausplexippusDPSCF300001ColeopteraDendroctonusponderosaeAPGK00000000.
19(INSDC)>Seq_1103023dna:supercontigsupercontig:DendPond_male_1.
0:Seq_1103023:1:4163268:1REFColeopteraDiabroticavirgiferaKZ688668HymenopteraDiachasmaalloeumNW_015144998HemipteraDiaphorinacitriNW_007377440HymenopteraDinoponeraquadricepsNW_014554123HymenopteraDiuraphisnoxiaNW_015368242DipteraDrosophilaalbomicansJH838198OrderSpeciesAccessionNotesDipteraDrosophilaamericanaCWKB01000001DipteraDrosophilaananassaeQMES00000000.
2(GenBank)>scaffold_13340dna:supercontigsupercontig:dana_caf1:scaffold_13340:1:23697760:1REFDipteraDrosophilaarizonaeNW_017127682DipteraDrosophilabiarmipesNW_016028565DipteraDrosophilabipectinataNW_016083910DipteraDrosophilabusckiiNC_030802DipteraDrosophilaelegansNW_016078481DipteraDrosophilaerectaQMER00000000.
2(GenBank)>scaffold_4929dna:supercontigsupercontig:dere_caf1:scaffold_4929:1:26641161:1REFDipteraDrosophilaeugracilisNW_016073535DipteraDrosophilaficusphilaNW_016067781DipteraDrosophilagrimshawiGCF_000005155.
2>scaffold_15110dna:supercontigsupercontig:dgri_caf1:scaffold_15110:1:24565398:1REFDipteraDrosophilahydeiNW_019378586DipteraDrosophilakikkawaiNW_016062640DipteraDrosophilamelanogasterGCF_000001625.
3>2Ldna:chromosomechromosome:BDGP6:2L:1:23513712:1REFDipteraDrosophilamirandaNC_030302.
1DipteraDrosophilamojavensisGCF_000005175.
2>scaffold_6540dna:supercontigsupercontig:dmoj_caf1:scaffold_6540:1:34148556:1REFDipteraDrosophilamontanaLUVX01000001DipteraDrosophilanasutaLYTC01000001DipteraDrosophilanavojoaNW_017181428DipteraDrosophilanovamexicanaQMEP01000001DipteraDrosophilaobscuraNW_019152098DipteraDrosophilapersimilisGCF_003286085.
1>scaffold_0dna:supercontigsupercontig:dper_caf1:scaffold_0:1:11822988:1REFDipteraDrosophilapseudoobscuraGCF_000001765.
3>2dna:chromosomechromosome:Dpse_3.
0:2:1:30819483:1REFDipteraDrosophilarhopaloaNW_016039821OrderSpeciesAccessionNotesDipteraDrosophilasechelliaGCF_004382195.
1>scaffold_0dna:supercontigsupercontig:dsec_caf1:scaffold_0:1:21120651:1REFDipteraDrosophilaserrataNW_018366212DipteraDrosophilasimulansGCF_000754195.
2>2Ldna:chromosomechromosome:ASM75419v3:2L:1:23539531:1REFDipteraDrosophilasubobscuraNGKO01001875DipteraDrosophilasuzukiiNW_016019885DipteraDrosophilatakahashiiNW_016034088DipteraDrosophilavirilisGCF_003285735.
1>scaffold_13049dna:supercontigsupercontig:dvir_caf1:scaffold_13049:1:25233164:1REFDipteraDrosophilawillistoniGCF_000005925.
1>scf2_1100000004943dna:supercontigsupercontig:dwil_caf1:scf2_1100000004943:1:16660200:1REFDipteraDrosophilayakubaGCF_000005975.
2>2Ldna:chromosomechromosome:dyak_caf1:2L:1:22324452:1REFPhasmatodeaDryococelusaustralisNKXL01000001HymenopteraDufoureanovaeangliaeNW_015373878EphemeropteraEphemeradanicaKZ497563DipteraEphydragracilisJXPQ01000001DipteraEristalisdimidiataJXPC01000001HymenopteraEufrieseamexicanaNW_016907855HymenopteraEuglossaDilemmaNIJG01000001HymenopteraEupelmusannulatusUDEW01000001HymenopteraEupelmusurozonusUELX01000001DipteraEutretadianaJXPB01000001HemipteraFerrisiavirgataFIZR01000001DipteraFopiusarisanusNW_011887740ThysanopteraFrankliniellaoccidentalisKZ555990LepidopteraGalleriamellonellaNTHM01000001HemipteraGerrisbuenoiKZ651037DipteraGlossinaausteniKK502400DipteraGlossinabrevipalpisKK351011OrderSpeciesAccessionNotesDipteraGlossinafuscipesGCA_000671735.
1>Scaffold0dna:supercontigsupercontig:GfusI1:Scaffold0:1:3329503:1DipteraGlossinamorsitansGCA_001014515.
1>scf7180000652170dna:supercontigsupercontig:GmorY1:scf7180000652170:1:25362821:1DipteraGlossinapallidipesGCA_000688715.
1>Scaffold0dna:supercontigsupercontig:GpalI1:Scaffold0:1:5836930:1DipteraGlossinapalpalisKN796171HymenopteraGoniozuslegneriNCVS01000001HymenopteraHabropodalaboriosaNW_017100116DipteraHaematobiairritansPGFW01000001HemipteraHalyomorphahalysNW_020110170HymenopteraHarpergnathossaltatorNW_020229871LepidopteraHeliconiuscydnoFAPN01000001LepidopteraHeliconiuselevatusFAPS01000001LepidopteraHeliconiusethillaFAPJ01000001LepidopteraHeliconiushecaleFAPG01000001LepidopteraHeliconiushecubaFAQH01000001LepidopteraHeliconiusheurippaFAPY01000001LepidopteraHeliconiushieraxFAQL01000001LepidopteraHeliconiusismeniusFAPP01000001LepidopteraHeliconiusmelpomeneHE671591LepidopteraHeliconiusnumataFASQ01000001LepidopteraHeliconiuspachinusFASS01000001LepidopteraHeliconiuspardalinusFASU01000001LepidopteraHeliconiustimaretaFAUB01000001LepidopteraHeliconiuswallaceiFAUM01000001LepidopteraHeliconiusxanthoclesFAUE01000001LepidopteraHelicoverpaarmigeraNW_018395390LepidopteraHelicoverpapunctigeraHpuns12202012Accessedfromi5kprojectOrderSpeciesAccessionNotesLepidopteraHelicoverpazeaKZ116068LepidopteraHeliothisvirescensNWSH01000001DipteraHermetiaillucensJXPW01000001DipteraHolcocephalafuscaJXPE01000001HemipteraHomalodiscavitripennisKZ826510ColeopteraHypothenemushampeiLBGY01000001PlecopteraIsoperlagrammaticaLVVW010000001LepidopteraLaparusdorisFAQD01000001HymenopteraLasioglossumalbipesANOB01000001HymenopteraLasiusnigerLBMM01000001OrthopteraLaupalakohalensisNNCF01000001PlecopteraLedniatumanaQKMV01000001HymenopteraLepidotrigonaventralisNIPQ01000001LepidopteraLeptideasinapsisFZQP01000001ColeopteraLeptinotarsadecemlineataNW_019289415HymenopteraLeptopilinaboulardiPQAT01000001HymenopteraLeptopilinaclavipesJUFY01000001LepidopteraLeremaacciusLGAG01000001OdonataLibellulafulvaGCA_000376725.
2TrichopteraLimnephiluslunatusKZ319399HymenopteraLinepithemahunileNW_012157838DipteraLiriomyzatrifoliiJXHJ01000001OrthopteraLocustamigratoriaAVCP010000001DipteraLuciliacuprinaJRES01001160refDipteraLuciliasericataJXPF01000001DipteraLutzomyialongipalpisJH689321ArchaeognathaMachilishrabeiGCA_003456935.
1OrderSpeciesAccessionNotesHemipteraMaconellicoccushirsutusFIZS01000001HymenopteraMacrocentruscingulumMVJL01000001LepidopteraMamestraconfigurataNDFZ01000001LepidopteraManducasextaJH668279refDipteraMayetioladestructorGL501517refPhasmatodeaMedauroideaextradentataPNEQ01000001HymenopteraMegachilerotundataNW_003797049DipteraMegaseliaabditaJXPG01000001DipteraMegaseliascalarisGCA_000341915.
2>scaffold9042dna:scaffoldscaffold:Msca1:scaffold9042:1:114435:1REFHymenopteraMegastigmusdorsalisUELU01000001HymenopteraMegastigmusstigmatizansUELV01000001HemipteraMelanaphissacchariNW_020270366HymenopteraMeliponaquadrifasciataKQ435686LepidopteraMelitaeacinxiaGCA_000716385.
1>scaffold1689dna:scaffoldscaffold:MelCinx1.
0:scaffold1689:1:668473:1REFStrepsipteraMengenillmoldrzykiAGDA01000001HymenopteraMicroplitisdemolitorNW_014463221DipteraMochlonyxcinctipesJXPH01000001HymenopteraMonomoriumpharaonisNW_012205351DipteraMuscadomesticaNW_004754939HemipteraMyzuspersicaeNW_019100468HymenopteraNasoniagiraultiGL273804HymenopteraNasonialongicornisGL277950HymenopteraNasoniavitripennisGCF_009193385.
2ref>1dna:chromosomechromosome:Nvit_2.
1:1:1:33571687:1REFDipteraNeobellieriabullataJXPI01000001HymenopteraNeodiprionleconteiNW_015380904LepidopteraNerudaaoedeFAPZ01000001OrderSpeciesAccessionNotesColeopteraNicrophorusvespilloidesNW_017095466HemipteraNilaparvatalugensNW_019105540HemipteraOncopeltusfasciatusKZ806866ColeopteraOnthophagustaurusNW_019280316HymenopteraOoceraeabiroiNW_011924876LepidopteraOperophterabrumataJTDY01000001HymenopteraOrmyrusnitidulusUCOL01000001HymenopteraOrmyruspomaceusUCOM01000001HymenopteraOrussusabietinusNW_019394231ColeopteraOryctesborbonicusLJIG01000001HemipteraPachypsyllavenustaKZ545295LepidopteraPapilioglaucusJWHW01000001LepidopteraPapiliomachaonNW_014478584LepidopteraPapiliomemnonBDMF01000001LepidopteraPapiliopolytesNW_013524711LepidopteraPapilioxuthusNW_013528584HemipteraParacoccusmarginatusFIZT01000001DipteraPaykulliamaculataNDXZ01000001PthirapteraPediculushumanusDS235882BlatoodeaPeriplanetaamericanaPGRX01000001HemipteraPhilaenusspumariusMPAX010000001DipteraPhlebotomuspapatasiJH660948LepidopteraPhoebissennaeLQNK01000631DipteraPhormiareginaMINK01000001DipteraPhorticavariegataJXPM01000001LepidopteraPierisrapaeNW_019093120HemipteraPiezodorusguildiniiJTEQ01000001LepidopteraPlodiainterpunctellaLT849405OrderSpeciesAccessionNotesLepidopteraPlutellaxylostellaNW_011952010HymenopteraPogomyrmexbarbatusNW_011929472ColeopteraPogonuschalceusCM008230HymenopteraPolistescanadensisNW_014569547HymenopteraPolistesdominulaNW_015148970ColeopteraPriacmaserrataAGRH01000001DipteraProctacanthuscoquillettiMNCL01000001HemipteraPseudococcuslongispinusFIZU01000001HymenopteraPseudomyrmexgracilisNW_018020584DipteraRhagoletiszephyriaNW_016157085HemipteraRhodniusprolixusKQ034056DipteraScaptodrosophilalebanonesisQMEN01000001HemipteraSchizaphisgraminumQEWZ01000001HemipteraSiphaflavaNW_020271713ColeopteraSitophilusoryzaePPTJ01000001HymenopteraSolenopsisinvictaGCF_000188075.
2>Si_gnG.
scaffold02694dna:supercontigsupercontig:Si_gnG:Si_gnG.
scaffold02694:1:6355204:1REFDipteraSphyracephalabrevicornisJXPL01000001LepidopteraSpodopterafrugiperdaNJHR01000001LepidopteraSpodopteralituraNC_036187DipteraStomoxyscalcitransNW_013171869HymenopteraSynergusumbraculusUCOK01000001DipteraTeleopsisdalmanniNLCU01024205HymenopteraTemnothoraxcurvispinosusNW_020209781DipteraTephritiscalifornicaJXPN01000001DipteraThemiraminorJXPZ01000001PhasmatodeaTimemacristinaeCM009472OrderSpeciesAccessionNotesDipteraTipulaoleraceaJXPP01000001HemipteraTrabutinamanniparaFKYK01000001HymenopteraTrachymyrmexcornetziNW_017280593HymenopteraTrachymyrmexseptentrionalisNW_017300354HymenopteraTrachymyrmexzetekiNW_017253421ColeopteraTriboliumcastaneumGCF_000002335.
3>LGXdna:chromosome_groupchromosome_group:Tcas5.
2:LGX:1:8676460:1REFHymenopteraTrichogrammapretiosumNW_019640787HymenopteraTrichomalopsissarcophagaeNNAY01000001LepidopteraTrichoplusianiGCF_003590095.
1HemipteraTrionymusperrisiiFIZV01000001DipteraTrupaneajonesiJXQA01000001LepidopteraVanessatameameaPEHJ01000001HymenopteraVollenhoviaemeryiNW_011953989HymenopteraWasmanniaauropunctataNW_012020309OrthopteraXenocatantopsbrachycerusOFSG01000001DipteraZaprionusindianusLWKS01000001DipteraZeugodacuscucurbitaeNW_011863665BlattodeaZootermopsisnevadensisGCF_000696155.
1>scaffold24dna:supercontigsupercontig:ZooNev1.
0:scaffold24:1:5111804:1REFSupplementalTable2.
Speciesandgenesequencesusedforconstructionofthephylogenetictree.
Accessionnumbersareincludedforeachofthe8genes(argk,COI,COII,cytb,ef1a,12S,18S,28S).
OnlyinclusiveofthosespecieswhichmettheBUSCOscorethreshold(0.
9or90%)SpeciesArgkCOICOIICytbEf1a12S18S28SAchalaruslyciadesMG646077.
1AcromyrmexechinatiorXM_011066862.
1AcyrthosiphonpisumXM_008187305.
2AB470712.
1FJ982392.
1FJ752022.
1FJ982418.
1JX965977.
1U27819.
1AedesaegyptiXM_001657339.
2KX420491.
1KC913582.
1AJ970959.
1KY000702.
1AY356400.
1U65375.
1AedesalbopictusXM_019692640.
1MF767287.
1AF324898.
1AJ971002.
1AF034471.
1HQ010437.
1AethinatumidaXM_020016979.
1KU179767.
1KC966688.
1KP133876.
1KP134008.
1AgrilusplanipennisXM_018470050.
2GU013563.
1XM_018479674.
2XM_018468835.
2AleocharabilineataKM847308.
1AmyeloistransitellaXM_013328496.
1AnophelesalnimanusAnophelesaquasalisAF548902.
1U92375.
1AF417769.
1AF417804.
1AnophelesarabiensisDQ465294.
1AF417741.
1AnophelesatroparvusGQ906807.
1KU877020.
1AM072973.
1LK054523.
1AnopheleschristyiAnophelescoluzziiMH025880.
1AnophelescracensDQ897948.
1AnophelesculicifaciesMH512896.
1EF208913.
1KP197030.
1KP893744.
1DQ173155.
1AnophelesdarlingiKP193458.
1U92383.
1AF417770.
1AF417805.
1AnophelesdirusDQ897946.
1JX070696.
1KF431914.
1AF417779.
1AF417814.
1AnophelesepiroticusAY789202.
1AnophelesfarautiKF202472.
1DQ674734.
1HQ840893.
1AF121065.
1AF121054.
1AF417815.
1AnophelesfunestusKU380404.
1AJ512749.
1DQ287361.
1DQ287369.
1AF417780.
1AF417816.
1AnophelesgambiaeDQ465336.
1AF417742.
1U35784.
1AM157179.
1KC177663.
1AnopheleskoliensisKU662306.
1U94304.
1HQ840920.
1AF121072.
1AF121061.
1AnophelesmaculatusEU256336.
1EF208910.
1AF440198.
1AY120851.
1AnophelesmelasU35794.
1AnophelesmerusU35791.
1AnophelesminimusKU497613.
1JQ042279.
1KF431913.
1AF293664.
1AF417781.
1DQ523567.
1AnophelesquadriannulatusKR014849.
1U35792.
1AnophelessinensisMG028766.
1KX840670.
1MG028854.
1AF293657.
1KJ617054.
1AnophelesstephensiMF124611.
1DQ026675.
1AF311254.
1AF034465.
1AF417784.
1AF417820.
1AnoplophoraglabripennisXM_018717621.
1AB439154.
1KF737699.
1KF142012.
1AntheraeayamamaiGU707195.
1AY037835.
1AphaenogasterashmeadiKP730068.
1AphaenogasterfloridanaKP730073.
1AphaenogasterfulvaKP730082.
1AphaenogastermiamianaKP730088.
1AphaenogasterpiceaKP730106.
1AphaenogasterrudisFJ824372.
1MG341765.
1EU658912.
1FJ824191.
1FJ824281.
1SpeciesArgkCOICOIICytbEf1a12S18S28SApisceranaXM_017049717.
1KT960839.
1EF467437.
1HM750231.
1ApisdorsataXM_006609081.
1KU752355.
1AF153126.
1KP259252.
1HM750230.
1ApisfloreaXM_012489495.
1AB284150.
1MG548267.
1KP259265.
1HQ108053.
1ApismelliferaAF023619.
1JN293758.
1EU513289.
1KC413715.
1AthaliarosaeXM_012404176.
2KF642888.
1KF528568.
1AB064266.
1AttacephalotesXM_012202340.
1MG948895.
1AttacolombicaXM_018192232.
1EU847939.
1EU448260.
1KF500036.
1BactroceradorsalisXM_011214398.
2KX051723.
1EU926791.
1JF521166.
1GU339154.
1U01251.
1BactroceralatifronsXM_018934259.
1MF140511.
1AB687549.
1MG683593.
1BactroceraoleaeXM_014233915.
1KY111527.
1MG683538.
1AY037337.
1BactroceratryoniMH135262.
1MG020808.
1JQ420932.
1MG683620.
1AY037345.
1BelgicaantarcticaJQ672717.
1DQ507305.
1DQ459549.
1BemisiatabaciXM_019046375.
1MF289534.
1BicyclusanynanaXM_024078988.
1KR139591.
1AY040163.
1KM923784.
1BlattellagermanicaFJ855501.
1S72627.
1DQ874268.
1KY014629.
1AY536379.
1FJ806322.
1BombusimpatiensXM_012391881.
2GU806825.
1AF281169.
1BombusterrestrisXM_003401454.
3JQ843670.
1X90406.
1KP670308.
1BombyxmoriXM_021351952.
1AB649195.
1AY343547.
1NM_001044045.
1AY037827.
1KF982847.
1CalephelisnemesisCalephelisvirginiensisCalopteryxsplendensDQ411707.
1DQ008208.
1CalycopiscecropsGU089704.
1CamponotusfloridanusXM_025406946.
1AF186362.
1CatajapyxaquilonarisEU368600.
1EF199978.
2CecidostibafungosaHM574094.
1JQ417157.
1CecidostibasemifasciaHM574093.
1CephuscinctusXM_015732313.
2HM432906.
1XM_015752756.
2CeratinacalcarataXM_018034016.
2KJ166268.
1GU321567.
1AY995679.
1CeratitiscapitataXM_004526740.
3MG764540.
1DQ011889.
1DQ006914.
1MG683583.
1KC177300.
1CeratosolensolmsiXM_011506811.
1AY842421.
1ChironomusripariusKR756187.
1DQ657920.
1AY820919.
1ChironomustentansKY225374.
1AF109712.
1CimexlectulariusXM_014383694.
2KR044731.
1XM_014405864.
1KJ461298.
1ClitarchushookeriGU194457.
1KY263955.
1CluniomarinusLN851838.
1CoboldiafuscipesMG296087.
1KC177601.
1KC177484.
1KC177282.
1KC177651.
1CopidosomafloridanumXM_014364357.
2KR877543.
1KF850043.
1CotesiavestalisKY832157.
1KX604901.
1KX605227.
1JX399880.
1AJ535953.
1CryptotermessecundusXM_023849251.
1EF442718.
1AF189120.
1DQ441676.
1DQ882635.
1CulexquinquefasciatusXM_001842457.
1KR768063.
1JQ716608.
1HQ709409.
1HM807289.
1CulicoidessonorensisKR683671.
1KP310110.
1CyphomyrmexcostatusXM_018545298.
1DanausplexippusEU141261.
1KT133515.
1KX110382.
1KP007541.
1DQ157894.
1AF392051.
1AF454921.
1GQ229491.
1DendroctonusponderosaeXM_019909387.
1JF888167.
1KJ531053.
1DiabroticavirgiferaXM_028298329.
1MH323083.
1AY533603.
1AY243734.
2DiachasmaalloeumXM_015265737.
1KR890748.
1SpeciesArgkCOICOIICytbEf1a12S18S28SDinoponeraquadricepsXM_014611839.
1DrosophilaamericanaJN019935.
1AY646740.
1GU299316.
1EU188739.
1JF735897.
1DrosophilaananassaeMG557557.
1KM487042.
1EU601719.
1EU189427.
1XR_046314.
1JF735891.
1DrosophilaarizonaeXM_018018451.
1MF443102.
1DQ436072.
1DrosophilabiarmipesXM_017097033.
1AY098456.
1AF474094.
1DrosophilabipectinataXM_017247855.
1AB032131.
1GQ376042.
1KM487044.
1DrosophilabusckiiXM_017991261.
1MF882465.
1KF601930.
1DrosophilaelegansXM_017266287.
1AB032130.
1AF461307.
1DrosophilaerectaXM_001971531.
3KX771108.
1GQ244453.
1JX500968.
1DrosophilaeugracilisXM_017228296.
1AY098461.
1AF474079.
1DrosophilaficusphilaXM_017198069.
1AB032133.
1AY757273.
1DrosophilagrimshawiGU597459.
1GU597491.
1EU189429.
1GU597390.
1DrosophilahydeiXM_023320454.
1MG086288.
1AF145888.
1DrosophilakikkawaiXM_017176053.
1KY973965.
1KP731163.
1DrosophilamelanogasterU26939.
1KC750827.
1GQ376046.
1AM403328.
1NM_079872.
4X97155.
1NR_133559.
1NR_133562.
1DrosophilamirandaXM_017285861.
1U51608.
1M95148.
1EF216276.
1XM_017287623.
1DrosophilamojavensisDQ383730.
1AY437281.
1EU494122.
1EU494550.
1XR_047783.
1GQ244442.
1DrosophilamontanaMG080424.
1DQ426799.
1JF735907.
1DrosophilanasutaGU597466.
1AB932783.
1EU494105.
1AF332704.
1GU597436.
1DrosophilanovamexicanaJF735929.
1JF735934.
1AY646768.
1AF184036.
1DrosophilaobscuraXM_022363945.
1U51614.
1AF081356.
1EF216277.
1XM_022368481.
1DrosophilapersimilisXM_002016998.
2AF451101.
1M95143.
1EF216278.
1XM_026990083.
1DrosophilapseudoobscuraAF451073.
1M95150.
1EF216279.
1AF220088.
1XR_053284.
1AF184037.
1DrosophilarhopaloaXM_017113570.
1XM_017136819.
1DrosophilasechelliaKJ426007.
1GQ244458.
1XR_048770.
1DrosophilaserrataXM_020962843.
1HQ631573.
1GQ376043.
1XM_020948685.
1DrosophilasimulansKT112992.
1GQ222022.
1AY037174.
1HQ110541.
1DrosophilasubobscuraMG082253.
1AF187689.
1EF216282.
1AF220094.
1EF216305.
1DrosophilasuzukiiXM_017078652.
1AB032128.
1LN867083.
1DrosophilatakahashiiXM_017144515.
1JF735911.
1AF474089.
1DrosophilavirilisMH423345.
1HQ110559.
1AY646772.
1AY560540.
1XR_049301.
1AF184035.
1DrosophilawillistoniXM_023178113.
1KT194321.
1HQ110560.
1EU494155.
1X97154.
1DrosophilayakubaDQ382818.
1M26990.
1HQ110545.
1DufoureanovaeangliaeXM_015577015.
1HM414187.
1EphemeradanicaKY261917.
1KF855909.
1KF855788.
1KF855833.
1KF855809.
1EphydragracilisEufrieseamexicanaXM_017898377.
1EuglossaDilemmaEupelmusannulatusMG343741.
1KR348775.
1EupelmusurozonusKR348766.
1KR348814.
1FerrisiavirgataKJ530607.
1LC278436.
1HQ893809.
1AY426079.
1LC278437.
1FopiusarisanusXM_011304607.
1KY689110.
1KC753514.
1KC594927.
1FrankliniellaoccidentalisMH319815.
1MG365569.
1KY775411.
1KJ916245.
1GU148015.
1JX002704.
1XM_026437035.
1GalleriamellonellaXM_026900087.
1MK187421.
1M83967.
1AF286298.
1GerrisbuenoiMG406973.
1KX821870.
1KX821900.
1KX821796.
1KX821825.
1KX821840.
1GlossinaausteniEU591834.
1EF531133.
1SpeciesArgkCOICOIICytbEf1a12S18S28SGlossinabrevipalpisEU591869.
1MK034891.
1EF531134.
1GlossinafuscipesMK094173.
1EU562281.
1MK034887.
1GlossinamorsitansEF531200.
1KR820741.
1KC177594.
1MK034895.
1KC177312.
1KC177834.
1GlossinapallidipesHQ702375.
1MK034890.
1EF531136.
1GlossinapalpalisKF597291.
1KJ013528.
1MK034896.
1AF322431.
1EF531137.
1GoniozuslegneriGU213948.
1HabropodalaboriosaXM_017938075.
1KY321466.
1HalyomorphahalysXM_014425440.
2KY570297.
1KY710681.
1KF273412.
1HarpergnathossaltatorXM_011149430.
3HeliconiusmelpomeneKP073002.
1EU449025.
1DQ448447.
1HelicoverpaarmigeraJN185455.
1KP210095.
1HQ677778.
1EF410045.
1AF389421.
1KT945996.
1HelicoverpapunctigeraKX422509.
1EF410058.
1HelicoverpazeaHM068068.
1GU438926.
1HQ677777.
1HQ177612.
1HQ178018.
1KT946005.
1HypothenemushampeiKX818264.
1LasioglossumalbipesGU706031.
1LasiusnigerEU142995.
1MG344523.
1MF993323.
1JQ681115.
1KT443340.
1LepidotrigonaventralisDQ813047.
1FJ042126.
1LeptideasinapsisLeptinotarsadecemlineataXM_023158001.
1AY165708.
1LeptopilinaboulardiKY077405.
1LeptopilinaclavipesKR899847.
1LeremaacciusGU089979.
1LibellulafulvaAF195745.
1DQ008205.
1FJ596602.
1LinepithemahunileXM_012359713.
1FJ161727.
1EF363097.
1AH012894.
2LuciliacuprinaXM_023444944.
1KX053871.
1DQ345111.
1DQ407477.
1MaconellicoccushirsutusKY373171.
1KY678264.
1AY426033.
1AY427403.
1MacrocentruscingulumJF963516.
1HQ177822.
1HQ177281.
1ManducasextaJN678236.
1U88190.
1MayetioladestructorMG130753.
1AF488423.
1MH282938.
1KJ917326.
1KC177649.
1MegachilerotundataXM_012291469.
1MG351078.
1MegastigmusdorsalisAY317240.
1AY898702.
1AY317197.
1AY317161.
1MegastigmusstigmatizansAY898703.
1MelanaphissacchariXM_025334412.
1MG838315.
1KT376991.
1MeliponaquadrifasciataEU163050.
1FJ975764.
1EF529493.
1MicroplitisdemolitorXM_008546537.
2HM904940.
1DQ538655.
1MochlonyxcinctipesMonomoriumpharaonisXM_012676742.
2KC419419.
1JQ695802.
1MuscadomesticaXM_011296089.
2MG091420.
1HM016775.
1DQ657064.
1DQ657113.
1AY573084.
1GQ465780.
1MyzuspersicaeXM_022308072.
1KY509874.
1AF143592.
1HQ528298.
1EF419315.
1JX965999.
1NasoniagiraultiEF638434.
1NasonialongicornisFJ554529.
1NasoniavitripennisXM_016989842.
1EF638430.
1GQ410677.
1NeodiprionleconteiXM_015666009.
1XM_015656300.
1NicrophorusvespilloidesXM_017930012.
1MH115515.
1EU147362.
1NilaparvatalugensXM_022337262.
1AB325705.
1JX556844.
1HQ116528.
1JF773148.
1OncopeltusfasciatusMG406644.
1M83959.
1U15188.
1LN623664.
1SpeciesArgkCOICOIICytbEf1a12S18S28SOnthophagustaurusXM_023061824.
1MH020533.
1EU162431.
1XM_023055227.
1KC294268.
1DQ430885.
1OoceraeabiroiXM_011351977.
3OperophterabrumataMG466845.
1AF232887.
1OrmyrusnitidulusHM574027.
1JQ417107.
1OrmyruspomaceusHM574022.
1JQ417171.
1OrussusabietinusXM_012429232.
2KC976945.
1KF528558.
1JF505477.
1GQ410604.
1OryctesborbonicusKJ915151.
1PapilioglaucusEU141279.
1KM548369.
1PapiliomachaonXM_014507128.
1MK186611.
1LC189137.
1GU372637.
1PapiliopolytesXM_013277807.
1HQ962338.
1LC189134.
1HM246439.
1GU196787.
1PapilioxuthusNM_001312616.
1JQ922032.
1LC189138.
1JQ924447.
1NM_001312698.
1ParacoccusmarginatusKY373146.
1EU188580.
1AY427410.
1PaykulliamaculataMG967799.
1FJ025742.
1FJ025694.
1FJ025404.
1FJ025496.
1PediculushumanusKX054334.
1GU323333.
1FJ267441.
1MH429017.
1AY077775.
1PhoebissennaeMF547337.
1PhormiareginaGU013648.
1DQ345102.
1KF908169.
1AF262957.
1AF366685.
1PhorticavariegataPierisrapaeGU215936.
1JQ996397.
1GQ268364.
1JQ924443.
1GU372646.
1DQ150034.
1KC413860.
1PlodiainterpunctellaAJ315030.
1KT145708.
1KJ836335.
2PlutellaxylostellaJX390653.
1PogomyrmexbarbatusXM_011644610.
2AF202484.
1PogonuschalceusJN170715.
1KU918208.
1KJ371137.
1GU556144.
1GU556114.
1PolistescanadensisXM_014758359.
1AY663544.
1PolistesdominulaXM_015332541.
1KR880422.
1KJ147264.
1KJ147292.
1GU596549.
1ProctacanthuscoquillettiKT733476.
1PseudococcuslongispinusKY373111.
1JX677901.
1HQ893808.
1KT199023.
1KT199048.
1PseudomyrmexgracilisXM_020427193.
1KU985485.
1AY703529.
1RhagoletiszephyriaXM_017638032.
1MG825319.
1EU109172.
1U01267.
1RhodniusprolixusAF449138.
1AF421339.
1AF394519.
1AJ421962.
1ScaptodrosophilalebanonesisEU493686.
1HQ110572.
1EU494188.
1EU494586.
1HQ110555.
1SchizaphisgraminumMG512288.
1AF469802.
1AJ315874.
1JX966014.
1AH003128.
2SiphaflavaXM_025556687.
1KR044391.
1AF254094.
1SitophilusoryzaeKU494238.
1KX190772.
1AM946646.
1AY131070.
1SolenopsisinvictaXM_026137080.
1EF620560.
1AY334566.
1SpodopterafrugiperdaKC262642.
1MF197868.
1HQ677792.
1HQ177674.
1HQ178084.
1SpodopteralituraXM_022978992.
1MG783870.
1HQ177695.
1HQ178113.
1StomoxyscalcitransXM_013248543.
1MG097496.
1EU627744.
1EU851308.
1FJ025698.
1DQ656886.
1FJ025499.
1SynergusumbraculusDQ012655.
1GU386442.
1TeleopsisdalmanniKT186763.
1KC177592.
1KT186734.
1KC177302.
1KC177739.
1TemnothoraxcurvispinosusMF436500.
1MG340300.
1XM_025036124.
1TimemacristinaeEU251511.
1JN615409.
1TrabutinamanniparaEU188595.
1EU188518.
1TrachymyrmexcornetziXM_018522051.
1KT783624.
1XM_018521294.
1SpeciesArgkCOICOIICytbEf1a12S18S28STrachymyrmexseptentrionalisXM_018483182.
1EU561600.
1XM_018498567.
1TrachymyrmexzetekiXM_018447315.
1KM224757.
1XM_018460735.
1TriboliumcastaneumXM_962718.
3KU913057.
1KJ003215.
1NM_001114363.
1KJ002951.
1KP419322.
1TrichogrammapretiosumXM_014382207.
2KM998973.
1AY676062.
1DQ181936.
1TrichomalopsissarcophagaeTrichoplusianiXM_026882819.
1GU439174.
1HQ677815.
1AB125896.
1KY514086.
1VanessatameameaXM_026642261.
1HQ734891.
1VollenhoviaemeryiXM_012022523.
1MG255049.
1EF012949.
1XM_012025884.
1WasmanniaauropunctataXM_011709607.
1MF926000.
1KX585845.
1EF012950.
1ZaprionusindianusEF632372.
1EF632396.
1GU597433.
1ZeugodacuscucurbitaeMH751503.
1MG020794.
1MG683520.
1ZootermopsisnevadensisXM_022061494.
1MF358687.
1GQ922444.
1EU253931.
1EU253721.
1EU253799.
1SupplementalTable3.
Phylogenyfossilnodeconstraintsusedforphylogenyconstructionwiththemeansand95%confidenceintervals.
NodeMean2.
5%Quantile97.
5%QuantileRoot407.
0383431Hemiptera289.
0232346Ephemeroptera/Odonata353.
0296410Hymenoptera252.
0221283Diptera157.
0108206Lepidoptera147.
0116178Blattodea/Phasmatodea250.
8195307Coleoptera258.
0246270SupplementalTable4.
P-valuesforANOVAsfor2-6merandtotalmicrosatellitecontentbyorder.
WhileordersappearedtohavesignificancewithastandardANOVA,allsignificancewasremovedwhencorrectedforphylogenetichistory.
Thep-valuesshownforthephylogeneticallycorrectedANOVAarethemeanofthep-valuescalculatedusingeachofthe100treesfromourposteriordistribution.
Innocaseswereanysignificantp-valuesfoundontheindividualphylogeneticallycorrectedANOVASfromwhichthesemeanswerecalculated.
MeasureStandardANOVAPhylogeneticallycorrectedANOVA2mer6.
76E-60.
8673mer9.
58E-100.
6914mer9.
72E-80.
7825mer0.
0050.
9646mer9.
72E-80.
7742-6mer0.
04E-20.
932bp/Mbp0.
4870.
999SupplementalTable5.
P-valuesforANOVAsfor2-6merandtotalmicrosatellitecontentbycentromeretype.
Innocasesweremonocentricandholocentricspeciessignificantlydifferent.
Thep-valuesshownforthephylogeneticallycorrectedANOVAarethemeanofthep-valuescalculatedusingeachofthe100treesfromourposteriordistribution.
Innocaseswereanysignificantp-valuesfoundontheindividualphylogeneticallycorrectedANOVAsfromwhichthesemeanswerecalculated.
MeasureStandardANOVAPhylogeneticallycorrectedANOVA2mer0.
1680.
2543mer0.
1750.
2654mer0.
3640.
4585mer0.
7170.
7716mer0.
8070.
8412-6mer0.
1850.
270Bp/Mbp0.
1090.
187