Chr966456.com
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Chromosome500000001000000001500000002000000000246810111359ChromosomalpositionSupplementaryFig.
1.
ChromosomaldistributionofS-pollenF-box-likesequencesintheC.
canephoragenome.
P.
aviumSFB3(AY571665)C.
canephorachr7(25880175-25878976)P.
mumeSLFL1(AB280956)M.
domesticaS3-SFBB3(AB539845)P.
integrifoliaS2-SLFlike1(KJ670463;AIK66486)P.
integrifoliaS2-SLF12(KJ670433;AIK66456)C.
canephorachr5(15839946-15838846);chr5(15846970-15845870)C.
canephorachr8(1899562-1898178)C.
canephorachr8(2902887-2901388)C.
canephorachr1(3389027-3387528)C.
canephorachr0(193221821-193220322)C.
canephorachr0(107197079-107195606)C.
canephorachr5(13485490-13486774)C.
canephorachr5(14885078-14883782)C.
canephorachr2(6654677-6656146)C.
canephorachr2(6657840-6658958)C.
canephorachr11(24018285-24016986)A.
hispanicumS1E-SLF(AJ515535)C.
canephorachr6(4568475-4567276)C.
canephorachr0(46856230-46855124)C.
canephorachr2(48711027-48712526)C.
canephorachr2(48652458-48653757)C.
canephorachr2(48703204-48701865)C.
canephorachr2(48661546-48660226)C.
canephorachr2(20793779-20792409)Cc02_g23650C.
canephorachr9(14737823-14736351)C.
canephorachr2(35580049-35578721)C.
canephorachr1(26012466-26011258)C.
canephorachr6(33994090-33995389)C.
canephorachr6(11614737-11613371)C.
canephorachr0(193457890-193459389)C.
canephorachr10(20687304-20685905)C.
canephorachr10(20652059-20650760)C.
canephorachr11(25166306-25167655)C.
canephorachr11(25129308-25130707)C.
canephorachr11(25168966-25170265)C.
canephorachr11(25132283-25133582)C.
canephorachr11(25182303-25180814)C.
canephorachr11(25175387-25173940)C.
canephorachr0(1548850-1550339)C.
canephorachr0(1571024-1572483)C.
canephorachr6(33998936-33997453)C.
canephorachr6(34168804-34170248)C.
canephorachr6(34181193-34182454)C.
canephorachr0(1693660-1692162)C.
canephorachr0(1685863-1684364)C.
canephorachr6(34104936-34103519)C.
canephorachr0(94010257-94008958)C.
canephorachr6(34229926-34231425)C.
canephorachr0(1696049-1694578)C.
canephorachr6(34222625-34223924)C.
canephorachr6(33716234-33714735)C.
canephorachr6(34041395-34042894)C.
canephorachr6(34065379-34066878)C.
canephorachr6(33773719-33775190)C.
canephorachr1(4800675-4799176)C.
canephorachr2(24385378-24383973)C.
canephorachr6(11158074-11159422)C.
canephorachr2(36705273669228)C.
canephorachr1(2535411-2534112)C.
canephorachr6(10535147-10533848)C.
canephorachr4(12692464-12693825)Cc04_g11960C.
canephorachr0(87907955-87909454)C.
canephorachr4(12689514-12690813)C.
canephorachr0(32496137-32497069)C.
canephorachr4(12679431-12680730)C.
canephorachr4(12675608-12676959)C.
canephorachr4(12671949-12673378)C.
canephorachr4(12661487-12662786)C.
canephorachr0(87940689-87942188)C.
canephorachr4(12686034-12687433)C.
canephorachr0(57515980-57514581)C.
canephorachr11(1606585-1605086)C.
canephorachr11(1601686-1600430)Cc11_g00560C.
canephorachr3(404004-405418)C.
canephorachr10(17318817-17320064)Cc10_g11210C.
canephorachr10(17295674-17296873)C.
canephorachr3(6921013-6922512)C.
canephorachr4(17598435-17599934)C.
canephorachr3(23351405-23352777)C.
canephorachr3(5457893-5459140)C.
canephorachr3(5461580-5462964)C.
canephorachr3(26351892-26350516)C.
canephorachr7(11788676-11787377)C.
canephorachr7(11794953-11793584)C.
canephorachr7(11773918-11772619)C.
canephorachr3(6423529-6422261)C.
canephorachr3(6427605-6426251)C.
canephorachr3(5790031-5791287)C.
canephorachr3(5826733-5827989)C.
canephorachr10(19556757-19558105)C.
canephorachr3(6431828-6430548)C.
canephorachr3(12574015-12572586)C.
canephorachr3(12581767-12580547)C.
canephorachr3(12577158-12575915)C.
canephorachr3(29023588-29024778)C.
canephorachr3(29009645-29011053)C.
canephorachr0(49697823-49696524)C.
canephorachr0(49705370-49704071)C.
canephorachr3(6947102-6945739)C.
canephorachr3(23357427-23358916)C.
canephorachr5(2606408-2607707)C.
canephorachr3(6930822-6929623)C.
canephorachr3(6940974-6939775)C.
canephorachr6(14658988-14657876)Cc06_g16460C.
canephorachr369255896924480)Cc03_g07530C.
canephorachr2(14049442-14050941)C.
canephorachr3(26745080-26746379)C.
canephorachr9(606171-607385)C.
canephorachr9(597529-598838)C.
canephorachr0(8380632-8379271)C.
canephorachr2(43512411-43513810)C.
canephorachr0(123830968-123832267)C.
canephorachr6(7941494-7942839)C.
canephorachr11(16995892-16997191)C.
canephorachr11(17004941-17006337)C.
canephorachr11(17917353-17918652)C.
canephorachr11(18029167-18030466)C.
canephorachr11(17838449-17839758)C.
canephorachr3(8306687-8308071)C.
canephorachr11(6782592-6781293)C.
canephorachr3(8315816-8317115)C.
canephorachr3(8318372-8319777)C.
canephorachr3(8310810-8312109)C.
canephorachr3(8326165-8327564)C.
canephorachr3(8329132-8330420)C.
canephorachr5(5300205-5298906)C.
canephorachr5(1150651-1152150)C.
canephorachr8(20089590-20090983)C.
canephorachr5(574442-575745)C.
canephorachr0(172558930-172557705)C.
canephorachr3(4873496-4872049)C.
canephorachr3(5094971-5096445)C.
canephorachr3(19295738-19297060)C.
canephorachr3(4864995-4863696)C.
canephorachr3(4870172-4868873)C.
canephorachr2(40418981-40417582)C.
canephorachr11(16278427-16277128)C.
canephorachr4(3068712-3069911)C.
canephorachr11(24030212-24028869)C.
canephorachr0(168517640-168516430)C.
canephorachr11(28396673-28397926)C.
canephorachr11(23792967-23794465)C.
canephorachr11(23788468-23789767)C.
canephorachr11(23783892-23785191)C.
canephorachr11(23779693-23781155)C.
canephorachr11(23262063-23263444)C.
canephorachr2(7060347-7061846)C.
canephorachr1(26012652-26011353)C.
canephorachr3(23874677-23873278)C.
canephorachr3(29988041-29989539)C.
canephorachr3(30006907-30008399)C.
canephorachr0(175831118-175830205);chr3(29993102-29994015)C.
canephorachr7(11712121-11710802)C.
canephorachr7(11715197-11713801)C.
canephorachr7(11707987-11706582)C.
canephorachr7(11702650-11701448)C.
canephorachr7(11699880-11698596)C.
canephorachr7(11994174-11995558)C.
canephorachr7(11168040-11169361)C.
canephorachr7(12747253-12745754)C.
canephorachr3(8284879-8283580)C.
canephorachr3(8261243-8259944)C.
canephorachr3(8249843-8248544)C.
canephorachr8(9480584-9481883)C.
canephorachr8(9430751-9432081)C.
canephorachr8(9457967-9459266)C.
canephorachr11(11160909-11159410)C.
canephorachr11(26516581-26517880)C.
canephorachr11(11173581-11172082)C.
canephorachr11(26477635-26478934)C.
canephorachr2(49973180-49971721)C.
canephorachr2(49493467-49494766)C.
canephorachr0(164241516-164242815)C.
canephorachr0(44441282-44442687)C.
canephorachr6(12271381-12270182)C.
canephorachr2(21299214-21300613)C.
canephorachr4(6976334-6975135)C.
canephorachr5(13675469-13676768)C.
canephorachr5(21171675-21170398)C.
canephorachr5(21156153-21157289)Cc05_g06390C.
canephorachr5(23225337-23223841)C.
canephorachr5(23220926-23219655)C.
canephorachr5(23229981-23228704)Cc05_g08700C.
canephorachr5(23237397-23238696)C.
canephorachr5(23330193-23328737)C.
canephorachr0(21150319-21151818)C.
canephorachr0(21098275-21099641)C.
canephorachr2(48670741-48669442)C.
canephorachr0(150263925-150265224)SupplementaryFig.
2.
BayesianphylogenyshowingtherelationshipoftheC.
canephoraS-pollenF-box-likesequencesandthereferencesequences(P.
aviumSFB3(AY571665);P.
mumeSLFLlike1(AB280956);M.
domesticaSFBB3(AB539845);P.
integrifoliaS2-SLF12(KJ670433-AIK66456);P.
integrifoliaS2SLF-like1(KJ670463-AIK66486),andA.
hispanicumSLF-S1E(AJ515535)).
Putativenon-functional(pseudogene)sequencesareunderlined.
Onlyposteriorcredibilityvaluesabove70areshown.
chrstandsforchromosome.
SequencescolouredinbluearetheputativeC.
canephoraS-pollencandidates.
P.
aviumSFB3(AY571665)C.
canephoraChr7(11712121-11710802)C.
canephoraChr7(11702650-11701448)C.
canephoraChr4(12692464-12693825)Cc04_g11960C.
canephoraChr3(6423529-6422261)C.
canephoraChr3(5790031-5791287)C.
canephoraChr3(5826733-5827989)C.
canephoraChr3(6431828-6430548)C.
canephoraChr3(12581767-12580547)C.
canephoraChr3(29023588-29024778)C.
canephoraChr6(14658988-14657876)Cc06_g16460C.
canephoraChr3(6925589-6924480)Cc03_g07530C.
canephoraChr3(5457893-5459140)C.
canephoraChr3(26351892-26350516)C.
canephoraChr3(19295738-19297060)C.
canephoraChr9(606171-607385)C.
canephoraChr0(8380632-8379271)C.
canephoraChr2(20793779-20792409)Cc02_g23650C.
canephoraChr9(14737823-14736351)C.
canephoraChr2(35580049-35578721)C.
canephoraChr11(25166306-25167655)C.
canephoraChr11(24030212-24028869)C.
canephoraChr11(1601686-1600430)Cc11_g00560C.
canephoraChr10(17318817-17320064)Cc10_g11210C.
canephoraChr11(28396673-28397926)C.
canephoraChr5(21171675-21170398)C.
canephoraChr5(21156153-21157289)Cc05_g06390C.
canephoraChr5(23229981-23228704)Cc05_g08700C.
canephoraChr5(23220926-23219655)C.
canephoraChr0(46856230-46855124)C.
canephoraChr5(15839946-15838846)/Chr5(15846970-15845870)C.
canephoraChr2(6657840-6658958)A.
hispanicumSLF-S1E(AJ515535)P.
mumeSLFLlike1(AB280956)M.
domesticaSFBB3(AB539845)P.
integrifoliaS2-SLF12(KJ670433-AIK66456)P.
integrifoliaS2SLF-like1(KJ670463-AIK66486)SupplementaryFig.
3.
Bayesianphylogenyshowingtherelationshipofthefunctional(complete)C.
canephoraS-pollenF-box-likesequencesandthereferencesequences(P.
aviumSFB3[AY571665];P.
mumeSLFLlike1[AB280956];M.
domesticaSFBB3[AB539845];P.
integrifoliaS2-SLF12[KJ670433-AIK66456];P.
integrifoliaS2SLF-like1[KJ670463-AIK66486],andA.
hispanicumSLF-S1E[AJ515535]).
Onlyposteriorcredibilityvaluesabove70areshown.
ColouredinbluearetheS-pollencandidates.
M.
domesticaSH-RNase(AB032247)P.
pyrifoliaS9-RNase(AB104909)P.
pyrifoliaS4-RNase(AB009385)L.
peruvianumS3-RNase(X76065)N.
alataS1-RNase(RND63887)P.
hybridaS3-RNase(PHU07363)A.
hispanicumS2-RNase(X96465)A.
hispanicumS4-RNase(X96466)A.
hispanicumS3-RNase(AJ315593)C.
canephorachr5:13567555-13569232C.
canephorachr5:15837393-15838196;Cc05_g03730C.
canephorachr5:15921499-15922290;Cc05_g03770C.
andrambovatensisS8Shrt-RNase(JN035342)C.
millotiiS8-RNase(JN035333)C.
andrambovatensisS36-RNase(JN035344)C.
bonnieriS25-RNase(JN035353)C.
resinosaS25-RNase(JN035363)C.
bonnieriS26-RNase(JN035354)C.
perrieriS26-RNase(JN035332)C.
canephoraS40-RNase(JN035306)C.
millotiiS17-RNase(JN035335)C.
millotiiS18-RNase(JN035336)C.
montisS18-RNase(JN035348)C.
bonnieriS45-RNase(JN035356)C.
montissacriS34-RNase(JN035351)C.
tricalysioidesS39-RNase(JN035359)C.
andrambovatensisS37-RNase(JN035345)C.
millotiiS10-RNase(JN035334)C.
resinosaS53-RNase(JN035366)C.
tsirananaeS10-RNase(JN035320)C.
bonnieriS13-RNase(JN035352)C.
montisS13-RNase(JN035347)C.
perrieriS13-RNase(JN035329)C.
resinosaS13-RNase(JN035362)C.
eugenioidesS13-RNase(JN035326)C.
millotiiS19-RNase(JN035337)C.
tsirananaeS49-RNase(JN035318)C.
bonnieriS27-RNase(JN03535)C.
canephoraS43-RNase(JN035309)C.
millotiiS33-RNase(JN035339)C.
resinosaS1-RNase(JN035360)C.
brevipesS1-RNase(JN035313)C.
eugenioidesS1-RNase(JN035324)C.
montisS21-RNase(JN035349)C.
montisS9-RNase(JN035346)C.
tsirananaeS9-RNase(JN035319)C.
perrieriS12-RNase(JN035328)C.
resinosaS4-RNase(JN035361)C.
eugenioidesS4-RNase(JN035325)C.
andrambovatensisS4-RNase(JN035341)C.
montisS24-RNase(JN035350)C.
perrieriS15-RNase(JN035330)C.
resinosaS29-RNase(JN035365)C.
tricalysioidesS11-RNase(JN035357)C.
resinosaS28-RNase(JN035364)C.
tricalysioidesS38-RNase(JN035358)C.
psetdozangtebariaeS38-RNase(JN035310)C.
tsirananaeS6-RNase(JN035315)C.
tsirananaeS7-RNase(JN035316)C.
psetdozangtebariaeS7-RNase(JN035311)C.
canephoraS3-RNase(JN035305)C.
canephoraS41-RNase(JN035307)C.
canephorachr2:6617450-6620971;Cc02_g08300C.
canephoraS42-RNase(JN035308)P.
ebracteolatusS1-RNase(JN035312)C.
perrieriS16-RNase(JN035331)C.
millotiiS32-RNase(JN035338)P.
dulcisSM-RNase(DQ099895)P.
aviumS3-RNase(AJ298312)SupplementaryFig.
4.
BayesianphylogenyshowingtherelationshipofthefourgenomicC.
canephorasequences(inblueandbrown;thatshowoneintronandthatcodesforaputativeproteinwithanisoeletricpointabove9)withfunctionalS-RNasesfromPrunus(Rosaceae),Maleae(Rosaceae),Solanaceae,andPlantaginaceae,aswellaswiththeC.
canephoraputativeS-RNasessequencesthatshowevidenceforpositiveselection.
ThesequencecolouredinbrownshowshighsimilaritytoarelicC.
canephorasequence,andthusisnotacandidateS-RNase(seetextfordetails).
Onlyposteriorcredibilityvaluesabove70areshown.
InbracketsareGenBankaccessionnumbers.
1.
ChromosomaldistributionofS-pollenF-box-likesequencesintheC.
canephoragenome.
P.
aviumSFB3(AY571665)C.
canephorachr7(25880175-25878976)P.
mumeSLFL1(AB280956)M.
domesticaS3-SFBB3(AB539845)P.
integrifoliaS2-SLFlike1(KJ670463;AIK66486)P.
integrifoliaS2-SLF12(KJ670433;AIK66456)C.
canephorachr5(15839946-15838846);chr5(15846970-15845870)C.
canephorachr8(1899562-1898178)C.
canephorachr8(2902887-2901388)C.
canephorachr1(3389027-3387528)C.
canephorachr0(193221821-193220322)C.
canephorachr0(107197079-107195606)C.
canephorachr5(13485490-13486774)C.
canephorachr5(14885078-14883782)C.
canephorachr2(6654677-6656146)C.
canephorachr2(6657840-6658958)C.
canephorachr11(24018285-24016986)A.
hispanicumS1E-SLF(AJ515535)C.
canephorachr6(4568475-4567276)C.
canephorachr0(46856230-46855124)C.
canephorachr2(48711027-48712526)C.
canephorachr2(48652458-48653757)C.
canephorachr2(48703204-48701865)C.
canephorachr2(48661546-48660226)C.
canephorachr2(20793779-20792409)Cc02_g23650C.
canephorachr9(14737823-14736351)C.
canephorachr2(35580049-35578721)C.
canephorachr1(26012466-26011258)C.
canephorachr6(33994090-33995389)C.
canephorachr6(11614737-11613371)C.
canephorachr0(193457890-193459389)C.
canephorachr10(20687304-20685905)C.
canephorachr10(20652059-20650760)C.
canephorachr11(25166306-25167655)C.
canephorachr11(25129308-25130707)C.
canephorachr11(25168966-25170265)C.
canephorachr11(25132283-25133582)C.
canephorachr11(25182303-25180814)C.
canephorachr11(25175387-25173940)C.
canephorachr0(1548850-1550339)C.
canephorachr0(1571024-1572483)C.
canephorachr6(33998936-33997453)C.
canephorachr6(34168804-34170248)C.
canephorachr6(34181193-34182454)C.
canephorachr0(1693660-1692162)C.
canephorachr0(1685863-1684364)C.
canephorachr6(34104936-34103519)C.
canephorachr0(94010257-94008958)C.
canephorachr6(34229926-34231425)C.
canephorachr0(1696049-1694578)C.
canephorachr6(34222625-34223924)C.
canephorachr6(33716234-33714735)C.
canephorachr6(34041395-34042894)C.
canephorachr6(34065379-34066878)C.
canephorachr6(33773719-33775190)C.
canephorachr1(4800675-4799176)C.
canephorachr2(24385378-24383973)C.
canephorachr6(11158074-11159422)C.
canephorachr2(36705273669228)C.
canephorachr1(2535411-2534112)C.
canephorachr6(10535147-10533848)C.
canephorachr4(12692464-12693825)Cc04_g11960C.
canephorachr0(87907955-87909454)C.
canephorachr4(12689514-12690813)C.
canephorachr0(32496137-32497069)C.
canephorachr4(12679431-12680730)C.
canephorachr4(12675608-12676959)C.
canephorachr4(12671949-12673378)C.
canephorachr4(12661487-12662786)C.
canephorachr0(87940689-87942188)C.
canephorachr4(12686034-12687433)C.
canephorachr0(57515980-57514581)C.
canephorachr11(1606585-1605086)C.
canephorachr11(1601686-1600430)Cc11_g00560C.
canephorachr3(404004-405418)C.
canephorachr10(17318817-17320064)Cc10_g11210C.
canephorachr10(17295674-17296873)C.
canephorachr3(6921013-6922512)C.
canephorachr4(17598435-17599934)C.
canephorachr3(23351405-23352777)C.
canephorachr3(5457893-5459140)C.
canephorachr3(5461580-5462964)C.
canephorachr3(26351892-26350516)C.
canephorachr7(11788676-11787377)C.
canephorachr7(11794953-11793584)C.
canephorachr7(11773918-11772619)C.
canephorachr3(6423529-6422261)C.
canephorachr3(6427605-6426251)C.
canephorachr3(5790031-5791287)C.
canephorachr3(5826733-5827989)C.
canephorachr10(19556757-19558105)C.
canephorachr3(6431828-6430548)C.
canephorachr3(12574015-12572586)C.
canephorachr3(12581767-12580547)C.
canephorachr3(12577158-12575915)C.
canephorachr3(29023588-29024778)C.
canephorachr3(29009645-29011053)C.
canephorachr0(49697823-49696524)C.
canephorachr0(49705370-49704071)C.
canephorachr3(6947102-6945739)C.
canephorachr3(23357427-23358916)C.
canephorachr5(2606408-2607707)C.
canephorachr3(6930822-6929623)C.
canephorachr3(6940974-6939775)C.
canephorachr6(14658988-14657876)Cc06_g16460C.
canephorachr369255896924480)Cc03_g07530C.
canephorachr2(14049442-14050941)C.
canephorachr3(26745080-26746379)C.
canephorachr9(606171-607385)C.
canephorachr9(597529-598838)C.
canephorachr0(8380632-8379271)C.
canephorachr2(43512411-43513810)C.
canephorachr0(123830968-123832267)C.
canephorachr6(7941494-7942839)C.
canephorachr11(16995892-16997191)C.
canephorachr11(17004941-17006337)C.
canephorachr11(17917353-17918652)C.
canephorachr11(18029167-18030466)C.
canephorachr11(17838449-17839758)C.
canephorachr3(8306687-8308071)C.
canephorachr11(6782592-6781293)C.
canephorachr3(8315816-8317115)C.
canephorachr3(8318372-8319777)C.
canephorachr3(8310810-8312109)C.
canephorachr3(8326165-8327564)C.
canephorachr3(8329132-8330420)C.
canephorachr5(5300205-5298906)C.
canephorachr5(1150651-1152150)C.
canephorachr8(20089590-20090983)C.
canephorachr5(574442-575745)C.
canephorachr0(172558930-172557705)C.
canephorachr3(4873496-4872049)C.
canephorachr3(5094971-5096445)C.
canephorachr3(19295738-19297060)C.
canephorachr3(4864995-4863696)C.
canephorachr3(4870172-4868873)C.
canephorachr2(40418981-40417582)C.
canephorachr11(16278427-16277128)C.
canephorachr4(3068712-3069911)C.
canephorachr11(24030212-24028869)C.
canephorachr0(168517640-168516430)C.
canephorachr11(28396673-28397926)C.
canephorachr11(23792967-23794465)C.
canephorachr11(23788468-23789767)C.
canephorachr11(23783892-23785191)C.
canephorachr11(23779693-23781155)C.
canephorachr11(23262063-23263444)C.
canephorachr2(7060347-7061846)C.
canephorachr1(26012652-26011353)C.
canephorachr3(23874677-23873278)C.
canephorachr3(29988041-29989539)C.
canephorachr3(30006907-30008399)C.
canephorachr0(175831118-175830205);chr3(29993102-29994015)C.
canephorachr7(11712121-11710802)C.
canephorachr7(11715197-11713801)C.
canephorachr7(11707987-11706582)C.
canephorachr7(11702650-11701448)C.
canephorachr7(11699880-11698596)C.
canephorachr7(11994174-11995558)C.
canephorachr7(11168040-11169361)C.
canephorachr7(12747253-12745754)C.
canephorachr3(8284879-8283580)C.
canephorachr3(8261243-8259944)C.
canephorachr3(8249843-8248544)C.
canephorachr8(9480584-9481883)C.
canephorachr8(9430751-9432081)C.
canephorachr8(9457967-9459266)C.
canephorachr11(11160909-11159410)C.
canephorachr11(26516581-26517880)C.
canephorachr11(11173581-11172082)C.
canephorachr11(26477635-26478934)C.
canephorachr2(49973180-49971721)C.
canephorachr2(49493467-49494766)C.
canephorachr0(164241516-164242815)C.
canephorachr0(44441282-44442687)C.
canephorachr6(12271381-12270182)C.
canephorachr2(21299214-21300613)C.
canephorachr4(6976334-6975135)C.
canephorachr5(13675469-13676768)C.
canephorachr5(21171675-21170398)C.
canephorachr5(21156153-21157289)Cc05_g06390C.
canephorachr5(23225337-23223841)C.
canephorachr5(23220926-23219655)C.
canephorachr5(23229981-23228704)Cc05_g08700C.
canephorachr5(23237397-23238696)C.
canephorachr5(23330193-23328737)C.
canephorachr0(21150319-21151818)C.
canephorachr0(21098275-21099641)C.
canephorachr2(48670741-48669442)C.
canephorachr0(150263925-150265224)SupplementaryFig.
2.
BayesianphylogenyshowingtherelationshipoftheC.
canephoraS-pollenF-box-likesequencesandthereferencesequences(P.
aviumSFB3(AY571665);P.
mumeSLFLlike1(AB280956);M.
domesticaSFBB3(AB539845);P.
integrifoliaS2-SLF12(KJ670433-AIK66456);P.
integrifoliaS2SLF-like1(KJ670463-AIK66486),andA.
hispanicumSLF-S1E(AJ515535)).
Putativenon-functional(pseudogene)sequencesareunderlined.
Onlyposteriorcredibilityvaluesabove70areshown.
chrstandsforchromosome.
SequencescolouredinbluearetheputativeC.
canephoraS-pollencandidates.
P.
aviumSFB3(AY571665)C.
canephoraChr7(11712121-11710802)C.
canephoraChr7(11702650-11701448)C.
canephoraChr4(12692464-12693825)Cc04_g11960C.
canephoraChr3(6423529-6422261)C.
canephoraChr3(5790031-5791287)C.
canephoraChr3(5826733-5827989)C.
canephoraChr3(6431828-6430548)C.
canephoraChr3(12581767-12580547)C.
canephoraChr3(29023588-29024778)C.
canephoraChr6(14658988-14657876)Cc06_g16460C.
canephoraChr3(6925589-6924480)Cc03_g07530C.
canephoraChr3(5457893-5459140)C.
canephoraChr3(26351892-26350516)C.
canephoraChr3(19295738-19297060)C.
canephoraChr9(606171-607385)C.
canephoraChr0(8380632-8379271)C.
canephoraChr2(20793779-20792409)Cc02_g23650C.
canephoraChr9(14737823-14736351)C.
canephoraChr2(35580049-35578721)C.
canephoraChr11(25166306-25167655)C.
canephoraChr11(24030212-24028869)C.
canephoraChr11(1601686-1600430)Cc11_g00560C.
canephoraChr10(17318817-17320064)Cc10_g11210C.
canephoraChr11(28396673-28397926)C.
canephoraChr5(21171675-21170398)C.
canephoraChr5(21156153-21157289)Cc05_g06390C.
canephoraChr5(23229981-23228704)Cc05_g08700C.
canephoraChr5(23220926-23219655)C.
canephoraChr0(46856230-46855124)C.
canephoraChr5(15839946-15838846)/Chr5(15846970-15845870)C.
canephoraChr2(6657840-6658958)A.
hispanicumSLF-S1E(AJ515535)P.
mumeSLFLlike1(AB280956)M.
domesticaSFBB3(AB539845)P.
integrifoliaS2-SLF12(KJ670433-AIK66456)P.
integrifoliaS2SLF-like1(KJ670463-AIK66486)SupplementaryFig.
3.
Bayesianphylogenyshowingtherelationshipofthefunctional(complete)C.
canephoraS-pollenF-box-likesequencesandthereferencesequences(P.
aviumSFB3[AY571665];P.
mumeSLFLlike1[AB280956];M.
domesticaSFBB3[AB539845];P.
integrifoliaS2-SLF12[KJ670433-AIK66456];P.
integrifoliaS2SLF-like1[KJ670463-AIK66486],andA.
hispanicumSLF-S1E[AJ515535]).
Onlyposteriorcredibilityvaluesabove70areshown.
ColouredinbluearetheS-pollencandidates.
M.
domesticaSH-RNase(AB032247)P.
pyrifoliaS9-RNase(AB104909)P.
pyrifoliaS4-RNase(AB009385)L.
peruvianumS3-RNase(X76065)N.
alataS1-RNase(RND63887)P.
hybridaS3-RNase(PHU07363)A.
hispanicumS2-RNase(X96465)A.
hispanicumS4-RNase(X96466)A.
hispanicumS3-RNase(AJ315593)C.
canephorachr5:13567555-13569232C.
canephorachr5:15837393-15838196;Cc05_g03730C.
canephorachr5:15921499-15922290;Cc05_g03770C.
andrambovatensisS8Shrt-RNase(JN035342)C.
millotiiS8-RNase(JN035333)C.
andrambovatensisS36-RNase(JN035344)C.
bonnieriS25-RNase(JN035353)C.
resinosaS25-RNase(JN035363)C.
bonnieriS26-RNase(JN035354)C.
perrieriS26-RNase(JN035332)C.
canephoraS40-RNase(JN035306)C.
millotiiS17-RNase(JN035335)C.
millotiiS18-RNase(JN035336)C.
montisS18-RNase(JN035348)C.
bonnieriS45-RNase(JN035356)C.
montissacriS34-RNase(JN035351)C.
tricalysioidesS39-RNase(JN035359)C.
andrambovatensisS37-RNase(JN035345)C.
millotiiS10-RNase(JN035334)C.
resinosaS53-RNase(JN035366)C.
tsirananaeS10-RNase(JN035320)C.
bonnieriS13-RNase(JN035352)C.
montisS13-RNase(JN035347)C.
perrieriS13-RNase(JN035329)C.
resinosaS13-RNase(JN035362)C.
eugenioidesS13-RNase(JN035326)C.
millotiiS19-RNase(JN035337)C.
tsirananaeS49-RNase(JN035318)C.
bonnieriS27-RNase(JN03535)C.
canephoraS43-RNase(JN035309)C.
millotiiS33-RNase(JN035339)C.
resinosaS1-RNase(JN035360)C.
brevipesS1-RNase(JN035313)C.
eugenioidesS1-RNase(JN035324)C.
montisS21-RNase(JN035349)C.
montisS9-RNase(JN035346)C.
tsirananaeS9-RNase(JN035319)C.
perrieriS12-RNase(JN035328)C.
resinosaS4-RNase(JN035361)C.
eugenioidesS4-RNase(JN035325)C.
andrambovatensisS4-RNase(JN035341)C.
montisS24-RNase(JN035350)C.
perrieriS15-RNase(JN035330)C.
resinosaS29-RNase(JN035365)C.
tricalysioidesS11-RNase(JN035357)C.
resinosaS28-RNase(JN035364)C.
tricalysioidesS38-RNase(JN035358)C.
psetdozangtebariaeS38-RNase(JN035310)C.
tsirananaeS6-RNase(JN035315)C.
tsirananaeS7-RNase(JN035316)C.
psetdozangtebariaeS7-RNase(JN035311)C.
canephoraS3-RNase(JN035305)C.
canephoraS41-RNase(JN035307)C.
canephorachr2:6617450-6620971;Cc02_g08300C.
canephoraS42-RNase(JN035308)P.
ebracteolatusS1-RNase(JN035312)C.
perrieriS16-RNase(JN035331)C.
millotiiS32-RNase(JN035338)P.
dulcisSM-RNase(DQ099895)P.
aviumS3-RNase(AJ298312)SupplementaryFig.
4.
BayesianphylogenyshowingtherelationshipofthefourgenomicC.
canephorasequences(inblueandbrown;thatshowoneintronandthatcodesforaputativeproteinwithanisoeletricpointabove9)withfunctionalS-RNasesfromPrunus(Rosaceae),Maleae(Rosaceae),Solanaceae,andPlantaginaceae,aswellaswiththeC.
canephoraputativeS-RNasessequencesthatshowevidenceforpositiveselection.
ThesequencecolouredinbrownshowshighsimilaritytoarelicC.
canephorasequence,andthusisnotacandidateS-RNase(seetextfordetails).
Onlyposteriorcredibilityvaluesabove70areshown.
InbracketsareGenBankaccessionnumbers.
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