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Session:Dairy:FeedAdditivesI(poster)Day/Time/Location:Monday,July16,2012,7:30AM-9:30AM,NorthHallAB#M311DeterminingthebioavailabilityoflysineinAjiPro-Lusingtheplasmafreeaminoaciddoseresponsemethod.
N.
L.
Whitehouse*1,E.
S.
Fletcher1,A.
F.
Brito1,C.
G.
Schwab2,andI.
Shinzato3,1UniversityofNewHampshire,Durham,2SchwabConsultingLLC,Boscobe,3AjinomotoHeartlandInc.
,Chicago,IL.
SixmultiparousruminallycannulatedHolsteincowsaveraging108dinmilkwereusedina6*6Latinsquarestudywith7-dperiods.
Sampleswerecollectedduringthelast3d.
The6treatmentswere:1)control(0g/dl-Lys),2)30g/dofabomasallyinfusedLys,3)60g/dofabomasallyinfusedLys,4)30g/doffeedsupplementedLys,5)45g/doffeedsupplementedLys,and6)60g/doffeedsupplementedLys.
Anl-LysHClproductcontaining80%Lysandassumedtobe100%bioavailablewasusedfortheinfusions.
TheAjiPro-L(AjinomotoCo.
Inc.
,Tokyo),whichcontains40%Lys(asfedbasis)wasmixedwith454gofconcentrateandofferedtothecows1hbeforeeachofthe3dailyfeedings.
ThebasaldietwasformulatedtomeettheLysrequirementsandcontained36%cornsilage,14%grasssilage,3%alfalfahayand47%concentrate.
Fourbloodsampleswerecollectedfromthetailveinat2-hintervalsstartingat0700h;sampleswerecentrifuged,deproteinized,andcompositedbycow/daywithplasmastoredat80°CforAAanalysis.
DatawereanalyzedusingtheMIXEDandPROCREGproceduresofSAS.
Milkyieldandcomposition,andDMIwerenotdifferentamongtreatments.
LysinewastheonlyplasmaAAthatincreasedlinearly(P≤0.
05)inresponsetoinfusedorfeedsupplementedLys.
TheslopesfortheinfusedandfeedsupplementedLyswere0.
0224(r2=0.
98)and0.
0078(r2=0.
94),respectively,whentheconcentrationunityg/mLwasusedforexpressingtheconcentrationofplasmaLysrelativetothatoftotalAA.
WhentheconcentrationunityMwaschosentoexpressplasmaLysasaproportionoftotalAA,theslopeswereslightlylowerfortheinfused(0.
0183;r2=0.
97)andfeedsupplemented(0.
0068;r2=0.
93)treatments.
ThecalculatedbioavailabilitiesforAjiPro-Lusingthe2differentsetsofslopes(0.
0224and0.
0078g/mLvs.
0.
0183and0.
0068M)wererelativelysimilaraveraging35%(0.
0078/0.
0224*100)and37%(0.
0068/0.
0183*100),thusindicatingmarginalimpactofconcentrationunity(g/mLvs.
M)ontheoutcomeofLysbioavailabilityvaluesusingtheinvivoplasmaresponsemethod.
KeyWords:aminoacids,lysine,bioavailabilityNotes:Page1of5Abstracts5/18/2012http://www.
fass-abstracts.
org/newmyprogram/printabstracts.
asp
N.
L.
Whitehouse*1,E.
S.
Fletcher1,A.
F.
Brito1,C.
G.
Schwab2,andI.
Shinzato3,1UniversityofNewHampshire,Durham,2SchwabConsultingLLC,Boscobe,3AjinomotoHeartlandInc.
,Chicago,IL.
SixmultiparousruminallycannulatedHolsteincowsaveraging108dinmilkwereusedina6*6Latinsquarestudywith7-dperiods.
Sampleswerecollectedduringthelast3d.
The6treatmentswere:1)control(0g/dl-Lys),2)30g/dofabomasallyinfusedLys,3)60g/dofabomasallyinfusedLys,4)30g/doffeedsupplementedLys,5)45g/doffeedsupplementedLys,and6)60g/doffeedsupplementedLys.
Anl-LysHClproductcontaining80%Lysandassumedtobe100%bioavailablewasusedfortheinfusions.
TheAjiPro-L(AjinomotoCo.
Inc.
,Tokyo),whichcontains40%Lys(asfedbasis)wasmixedwith454gofconcentrateandofferedtothecows1hbeforeeachofthe3dailyfeedings.
ThebasaldietwasformulatedtomeettheLysrequirementsandcontained36%cornsilage,14%grasssilage,3%alfalfahayand47%concentrate.
Fourbloodsampleswerecollectedfromthetailveinat2-hintervalsstartingat0700h;sampleswerecentrifuged,deproteinized,andcompositedbycow/daywithplasmastoredat80°CforAAanalysis.
DatawereanalyzedusingtheMIXEDandPROCREGproceduresofSAS.
Milkyieldandcomposition,andDMIwerenotdifferentamongtreatments.
LysinewastheonlyplasmaAAthatincreasedlinearly(P≤0.
05)inresponsetoinfusedorfeedsupplementedLys.
TheslopesfortheinfusedandfeedsupplementedLyswere0.
0224(r2=0.
98)and0.
0078(r2=0.
94),respectively,whentheconcentrationunityg/mLwasusedforexpressingtheconcentrationofplasmaLysrelativetothatoftotalAA.
WhentheconcentrationunityMwaschosentoexpressplasmaLysasaproportionoftotalAA,theslopeswereslightlylowerfortheinfused(0.
0183;r2=0.
97)andfeedsupplemented(0.
0068;r2=0.
93)treatments.
ThecalculatedbioavailabilitiesforAjiPro-Lusingthe2differentsetsofslopes(0.
0224and0.
0078g/mLvs.
0.
0183and0.
0068M)wererelativelysimilaraveraging35%(0.
0078/0.
0224*100)and37%(0.
0068/0.
0183*100),thusindicatingmarginalimpactofconcentrationunity(g/mLvs.
M)ontheoutcomeofLysbioavailabilityvaluesusingtheinvivoplasmaresponsemethod.
KeyWords:aminoacids,lysine,bioavailabilityNotes:Page1of5Abstracts5/18/2012http://www.
fass-abstracts.
org/newmyprogram/printabstracts.
asp
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