Expression免费vps

LeaderinBiomolecularSolutionsforLifeScienceVPS37ARabbitpAbCatalogNo.
:A7853BasicInformationCatalogNo.
A7853ObservedMW44kDaCalculatedMW20kDa/41kDa/44kDaCategoryPrimaryantibodyApplicationsWB,IHC,IFCross-ReactivityHuman,Mouse,RatRecommendedDilutionsWB1:500-1:2000IHC1:50-1:200IF1:50-1:200Contact400-999-6126cn.
market@abclonal.
com.
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cnBackgroundThisgenebelongstotheVPS37family,andencodesacomponentoftheESCRT-I(endosomalsortingcomplexrequiredfortransportI)proteincomplex,requiredforthesortingofubiquitinatedtransmembraneproteinsintointernalvesiclesofmultivesicularbodies.
Expressionofthisgeneisdownregulatedinhepatocellularcarcinoma,andmutationsinthisgeneareassociatedwithautosomalrecessivespasticparaplegia-53.
Arelatedpseudogenehasbeenidentifiedonchromosome5.
Alternativelysplicedtranscriptvariantshavebeenfoundforthisgene.
ImmunogenInformationGeneIDSwissProt137492Q8NEZ2ImmunogenRecombinantfusionproteincontainingasequencecorrespondingtoaminoacids224-397ofhumanVPS37A(NP_689628.
2).
SynonymsVPS37A;HCRP1;PQBP2;SPG53ProductInformationSourceIsotypePurificationRabbitIgGAffinitypurificationStorageStoreat-20℃.
Avoidfreeze/thawcycles.
Buffer:PBSwith0.
02%sodiumazide,50%glycerol,pH7.
3.
ValidationDataAntibody|Protein|ELISAKits|Enzyme|NGS|ServiceForresearchuseonly.
Notfortherapeuticordiagnosticpurposes.
Pleasevisithttp://abclonal.
comforacompletelistingofrecommendedproducts.
Westernblotanalysisofextractsofvariouscelllines,usingVPS37Aantibody(A7853)at1:1000dilution.
_Secondaryantibody:HRPGoatAnti-RabbitIgG(H+L)(AS014)at1:10000dilution.
_Lysates/proteins:25ugperlane.
_Blockingbuffer:3%nonfatdrymilkinTBST.
_Detection:ECLEnhancedKit(RM00021).
_Exposuretime:30s.
Immunohistochemistryofparaffin-embeddedratbrainusingVPS37Aantibody(A7853)atdilutionof1:100(40xlens).
Immunohistochemistryofparaffin-embeddedmousestomachusingVPS37Aantibody(A7853)atdilutionof1:100(40xlens).