Doxycyclinedvdes-644
dvdes-644 时间:2021-01-20 阅读:()
SupplementaryTableS1.
ProductsusedforhumaniPSCsmaintenance.
ProductReferenceCompanyApplicationmTeSRPlus#05825STEMCELLTechnologiesiPSCmediumPenicillinStreptomycin(P/S)#15140122ThermoFisherScientificCellmediumMatrigel#354234CorningMatrixStemProAccutaseCellDissociationReagent(Accutase)#A1110501ThermoFisherScientificCellpassageThiazovivin(TZV)#72252STEMCELLTechnologiesiPSCsurvivalROCKinhibitor(RI)#Y-27632STEMCELLTechnologiesiPSCsurvivalTableS2.
Productsusedforlentiviralproduction.
ProductReferenceCompanyApplicationM2-rtTA(rtTA)#20342AddgeneViralproductionpTet-O-Ngn2-puro(Ngn2)#52047AddgeneViralproductionpMD2.
G#12259AddgeneViralproductionpRSV-Rev#12253AddgeneViralproductionpMDLg/pRRE#12251AddgeneViralproductiontetO-Sox9-Puro(Sox9)HenrikAhleniusgroupViralproductiontetO-Nfib-Hygro(Nfib)HenrikAhleniusgroupViralproductionDMEM#D5796SigmaVirusresuspensionTableS3.
ProductsusedforthegenerationofinducedneuronsandastrocytesfromiPSCs.
ProductReferenceCompanyApplicationDoxycycline(Dox)#10592-13-9ThermoFisherScientifictetOinductionHighPureRNAIsolationKit#11828665001RocheRNAextractionCellscrapers#179693LabClinicsNeuralpassagePFA4%#30525-89-4VWRCellfixationDMEM/F12#11330057ThermoFisherScientificNeuralandastrocytemediumNeurobasal#21103049ThermoFisherScientificNeuralandastrocytemediumB-27supplement#17504044ThermoFisherScientificNeuralandastrocytemediumN2supplement#17502048ThermoFisherScientificNeuralandastrocytemediumGlutaMAX#35050061ThermoFisherScientificNeuralandastrocytemediumPuromycine#A1113803ThermoFisherScientificCellselectionSterilefiltersof40mporus#352340CorningNeuralpassageFBS#16000044ThermoFisherScientificAstrocytemediumNEAA#11140035ThermoFisherScientificAstrocytemediumbFGF#100-18BPeprotechAstrocytemediumCNTF#450-13PeprotechAstrocytemediumBMP4#120-05ETPeprotechAstrocytemediumTableS4.
ProductsusedforsiRNAtransfection,immunocytochemistry,RT-qPCR,ELISAandproteinquantification.
ProductReferenceCompanyApplicationLipofectamineRNAiMAX#13778075ThermoFisherScientificsiRNAtransfectionsiRNASilencerSelect(siRNA)#4392420ThermoFisherScientificsiRNAtransfectionsiRNAEXTL2geneAssayID:si4899NegativeControlsiRNA#4459405ThermoFisherScientificsiRNAtransfectionTriton-X100#28817.
295VWRImmunocytochemistryNormalDonkeySerum#S30-100MMerckMilliporeImmunocytochemistryDAPIHoechst33342#D1306#H3570ThermoFisherScientificThermoFisherScientificImmunocytochemistryImmunocytochemistryMOWIOL#475904MerckMilliporeImmunocytochemistryHighCapacitycDNAReverseTranscriptionkit#4368814AppliedBiosystemsRT-qPCRRNaseInhibitor#N8080119AppliedBiosystemsRT-qPCRLightCycler480ProbesMaster#04887301001RocheRT-qPCRDCProteinAssay#5000111Bio-RadProteinquantificationTableS5.
Antibodiesusedforimmunocytochemistry.
ProductReferenceDilutionCompanyrabbitanti-TUJ1primaryantibody#MMS-435P1:500CovanceMouseanti-LAMP2primaryantibody#H4B4-c1:100HybridomaBankchickenanti-MAP2primaryantibody#ab53921:5000Abcammouseanti-VIMprimaryantibody#M07251:250Dakorabbitanti-S100Bprimaryantibody#2870031:400Synapticsystemsdonkeyanti-mouseCy2secondaryantibody#715-225-1501:200JacksonImmunoresearchdonkeyanti-rabbitCy3secondaryantibodydonkeyanti-chickenAF488secondaryantibodydonkeyanti-mouseAF488secondaryantibodydonkeyanti-rabbitAF568secondaryantibody#711-165-152#703-545-155#A-21202#A-100421:2001:2501:2501:250JacksonImmunoresearchJacksonImmunoresearchThermofisherScientificThermofisherScientificTableS6.
TaqManassaysusedforqPCR.
ProductReferenceCompanyTaqManGeneExpressionAssays(TaqManAssay)#4331182ThermoFisherScientificTaqManAssayGAPDHAssayID:Hs99999905_m1TaqManAssayNANOGAssayID:Hs02387400_g1TaqManAssayPOU5F1AssayID:Hs01654807_s1TaqManAssayTUBB3AssayID:Hs00801390_s1TaqManAssaySYPAssayID:Hs00300531_m1TaqManAssayMAP2AssayID:Hs00258900_m1TaqManAssayGFAPAssayID:Hs00909233_m1TaqManAssayS100BAssayID:Hs00902901_m1TaqManAssayALDH1L1AssayID:Hs00201836_m1TaqManAssayEXTL2AssayID:Hs00242124_m1
ProductsusedforhumaniPSCsmaintenance.
ProductReferenceCompanyApplicationmTeSRPlus#05825STEMCELLTechnologiesiPSCmediumPenicillinStreptomycin(P/S)#15140122ThermoFisherScientificCellmediumMatrigel#354234CorningMatrixStemProAccutaseCellDissociationReagent(Accutase)#A1110501ThermoFisherScientificCellpassageThiazovivin(TZV)#72252STEMCELLTechnologiesiPSCsurvivalROCKinhibitor(RI)#Y-27632STEMCELLTechnologiesiPSCsurvivalTableS2.
Productsusedforlentiviralproduction.
ProductReferenceCompanyApplicationM2-rtTA(rtTA)#20342AddgeneViralproductionpTet-O-Ngn2-puro(Ngn2)#52047AddgeneViralproductionpMD2.
G#12259AddgeneViralproductionpRSV-Rev#12253AddgeneViralproductionpMDLg/pRRE#12251AddgeneViralproductiontetO-Sox9-Puro(Sox9)HenrikAhleniusgroupViralproductiontetO-Nfib-Hygro(Nfib)HenrikAhleniusgroupViralproductionDMEM#D5796SigmaVirusresuspensionTableS3.
ProductsusedforthegenerationofinducedneuronsandastrocytesfromiPSCs.
ProductReferenceCompanyApplicationDoxycycline(Dox)#10592-13-9ThermoFisherScientifictetOinductionHighPureRNAIsolationKit#11828665001RocheRNAextractionCellscrapers#179693LabClinicsNeuralpassagePFA4%#30525-89-4VWRCellfixationDMEM/F12#11330057ThermoFisherScientificNeuralandastrocytemediumNeurobasal#21103049ThermoFisherScientificNeuralandastrocytemediumB-27supplement#17504044ThermoFisherScientificNeuralandastrocytemediumN2supplement#17502048ThermoFisherScientificNeuralandastrocytemediumGlutaMAX#35050061ThermoFisherScientificNeuralandastrocytemediumPuromycine#A1113803ThermoFisherScientificCellselectionSterilefiltersof40mporus#352340CorningNeuralpassageFBS#16000044ThermoFisherScientificAstrocytemediumNEAA#11140035ThermoFisherScientificAstrocytemediumbFGF#100-18BPeprotechAstrocytemediumCNTF#450-13PeprotechAstrocytemediumBMP4#120-05ETPeprotechAstrocytemediumTableS4.
ProductsusedforsiRNAtransfection,immunocytochemistry,RT-qPCR,ELISAandproteinquantification.
ProductReferenceCompanyApplicationLipofectamineRNAiMAX#13778075ThermoFisherScientificsiRNAtransfectionsiRNASilencerSelect(siRNA)#4392420ThermoFisherScientificsiRNAtransfectionsiRNAEXTL2geneAssayID:si4899NegativeControlsiRNA#4459405ThermoFisherScientificsiRNAtransfectionTriton-X100#28817.
295VWRImmunocytochemistryNormalDonkeySerum#S30-100MMerckMilliporeImmunocytochemistryDAPIHoechst33342#D1306#H3570ThermoFisherScientificThermoFisherScientificImmunocytochemistryImmunocytochemistryMOWIOL#475904MerckMilliporeImmunocytochemistryHighCapacitycDNAReverseTranscriptionkit#4368814AppliedBiosystemsRT-qPCRRNaseInhibitor#N8080119AppliedBiosystemsRT-qPCRLightCycler480ProbesMaster#04887301001RocheRT-qPCRDCProteinAssay#5000111Bio-RadProteinquantificationTableS5.
Antibodiesusedforimmunocytochemistry.
ProductReferenceDilutionCompanyrabbitanti-TUJ1primaryantibody#MMS-435P1:500CovanceMouseanti-LAMP2primaryantibody#H4B4-c1:100HybridomaBankchickenanti-MAP2primaryantibody#ab53921:5000Abcammouseanti-VIMprimaryantibody#M07251:250Dakorabbitanti-S100Bprimaryantibody#2870031:400Synapticsystemsdonkeyanti-mouseCy2secondaryantibody#715-225-1501:200JacksonImmunoresearchdonkeyanti-rabbitCy3secondaryantibodydonkeyanti-chickenAF488secondaryantibodydonkeyanti-mouseAF488secondaryantibodydonkeyanti-rabbitAF568secondaryantibody#711-165-152#703-545-155#A-21202#A-100421:2001:2501:2501:250JacksonImmunoresearchJacksonImmunoresearchThermofisherScientificThermofisherScientificTableS6.
TaqManassaysusedforqPCR.
ProductReferenceCompanyTaqManGeneExpressionAssays(TaqManAssay)#4331182ThermoFisherScientificTaqManAssayGAPDHAssayID:Hs99999905_m1TaqManAssayNANOGAssayID:Hs02387400_g1TaqManAssayPOU5F1AssayID:Hs01654807_s1TaqManAssayTUBB3AssayID:Hs00801390_s1TaqManAssaySYPAssayID:Hs00300531_m1TaqManAssayMAP2AssayID:Hs00258900_m1TaqManAssayGFAPAssayID:Hs00909233_m1TaqManAssayS100BAssayID:Hs00902901_m1TaqManAssayALDH1L1AssayID:Hs00201836_m1TaqManAssayEXTL2AssayID:Hs00242124_m1
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