sampleszpanel
zpanel  时间:2021-01-03  阅读:(
)
 
 
1SupplementaryMaterialfor:"ComparativeClinicalUtilityofTumorGenomicTestingandCell-FreeDNAinMetastaticBreastCancer"KaraN.
MaxwellMDPhD1,DanielleSoucier-Ernst2,EminTahirovicPhD3,AndreaB.
TroxelScD7,EricaCarpenterPhD1,2,ChristopherColameco2,CandaceClark2,MichaelFeldmanMDPhD4,BijalKakrecha1,MelissaLanger8,JoyLee2,DavidLewis2,DavidLiebermanMS4,JenniferJ.
D.
MorrissettePhD4,MattR.
PaulBS5,Tien-chiPanMS5,StephanieYee1,NatalieShih4,LewisA.
ChodoshMDPhD2,5,6,AngelaDeMicheleMDMSCE1,2,31DepartmentofMedicine,DivisionofHematology-Oncology;2AbramsonCancerCenter;3DepartmentofBiostatisticsandEpidemiology;4DepartmentofPathologyandLaboratoryMedicine;5DepartmentofCancerBiology;6DepartmentofMedicine,DivisionofEndocrinology,DiabetesandMetabolismatthePerelmanSchoolofMedicineattheUniversityofPennsylvania;7DepartmentofPopulationHealth,NYUSchoolofMedicine,8UniversityofMarylandSchoolofMedicineTableofContentsSupplementaryMethods…2SupplementaryFigure1…3SupplementaryFigure2…4SupplementaryFigure3…5SupplementaryFigure4…6SupplementaryTable1…7SupplementaryTable2…8SupplementaryTable3…9SupplementaryTable4…10SupplementaryTable5ExcelSupplementaryTable6…122SUPPLEMENTARYMETHODSDetailsonpatients,informedconsent,andstudydesignPatientsaged18andoverwitheithernewlysuspected,untreatedmetastaticbreastcancer,orprogressingdisease,wereeligibleiftheyhad:1)historyofhistologically-confirmedprimarybreastcancer;2)clinicalorimagingevidencesuggestiveofrecurrentmetastaticbreastcancerinalocal,regional,ordistantlocation;3)willingnesstoundergoand/orprovidetissuefromarecentbiopsyofrecurrenttumorforbothclinicalandresearchtesting;and4)willingnesstoundergobloodspecimencollection.
Patientswereexcludediftheywereonanticoagulationthatcouldnotbeinterruptedforthepurposeofstudyprocedures.
Genomictestingwaspaidforthroughinsurancecoverage,oraresearchfund.
Consentedpatientsunderwentclinicalbiopsyofametastaticsiteselectedbyreviewofallimagingbyaninterventionalradiologist(addinitials).
Selectionwasonthebasisofthemostsafelyaccessiblelesion.
Uptofourcorebiopsiesoftumorwereobtainedandanalyzedaccordingtoastandardalgorithmfor(1)histology,(2)estrogen,progesteroneandHer2receptorsbyASCO/CAPguidelines,and(3)CLIA-approvedtumorgenomictesting.
WholebloodsamplesweresentforprocessingandanalysisbyCLIA-approvedcfDNAgenomictesting.
Clinicaldatawereabstractedfromelectronicmedicalrecordsandprimaryreportsbytrainedresearchcoordinators.
SupplementaryFigure1:METAMORPHStudySchema.
DiagramoftheclinicalandresearchactivitiesintheMETAMORPHstudy.
PatientswithametastaticbreastcancerdiagnosisareinvitedtoenrollinMETAMORPH.
Theyundergoaclinicalbiopsy,aresearchblooddrawandanoptionalbonemarrowaspiration.
Theresearchblooddrawissentforfurtheranalysisofcirculatingtumorcells(CTC)anddisseminatedtumorcells(DTC)(datanotshown).
TheresearchblooddrawisalsosenttoGuardantHealthfortheGuardant360assay.
Thebiopsysampleissenttotheresearchtissuerepositoryforfurtheranalysisbywholeexomesequencing(WES)andRNAseq(datanotshown).
Thebiopsyisalsosentforsurgicalpathology(SurgPath)forreceptorstatusandgenomictumortestingattheCenterforPersonalizedDiagnosis(CPD).
3SupplementaryFigure2:METAMORPHConsortdiagram.
Seventypatientswereconsentedtothestudy.
Fourpatientsdidnotundergobiopsyduetoclinicianjudgementthatbiopsywouldnotbewarranted,providing66samplesfortumoranalysis.
Receptorswereobtainedfor62patientsandgenomictumorprofiling(CPDresults)wereobtainedfor53patients.
Receptorswerenotobtainedinsixpatientstherewasnotumorfoundonthehistologicalreviewforfivesubjectsandwasstillpendingforonesubjectatthetimeoftheanalysis.
CPDresultswerenotobtainedinninepatientsbecausefourhadnegativebiopsies,threefaileddecalcificationofbonematerial,andtwohadothersomatictestingperformed.
Threepatientsdidnotundergoblooddrawbecauseoflosstofollowuporrefusalofbiopsyproviding67samplesavailableforcfDNAanalysis.
Guardant360version1panelresultswereobtainedfor35patients.
Twenty-ninepatientsdidnothavecfDAsentforassayandthreehadtheversion2panelperformed.
Consentedtostudy(n=70)M0clinicalbiopsy(n=66)Reasonsnobx(n=4)M0Receptors(n=60)CPDResults(n=53)BloodsampleavailableforcfDNA(plasmaaliquots)(n=67)Guardantv1Results(n=35)Reasonsnobloodsample(n=3)ReasonsnoGuardantresults(n=32)ReasonsnoCPDresults(n=9)Reasonsnoreceptors(n=6)4140034150006140031150011140032140033140042150004150007140037140044140023140039140036140040150003150009140017140043150013150002HRStatus-PrimaryHRStatus-Metastasis#tumormutations/VUSsPatientIDPIK3CAHotspotPIK3CAOtherTP53ERBB2AKT1EGFRKITPDGFRAPTENHormoneReceptorStatusHR+Her2HRHer2+TNBCHR+Her2UnkA:AmplificationAAAAAAA341100000111111120212SupplementaryFigure3:Mutationalspectrainthetumorcohort.
Heatmapshowingthealterationsidentifiedbymetastatictumortestinginthetumorcohort.
Individualpatientidentifiers(ID)listedwithannotationsforreceptorstatusofprimary(ifapplicable)andofthebiopsiedmetastasis.
Totalnumberofalterationsidentifiedbyassay.
OnlyalterationsingenessharedbetweenCPD_FullandCPD_PPPareshown.
5a.
b.
SupplementaryFigure4:Analysisoftimetoprogressiononstandardtreatmentstratifiedbygenomicbiomarkers.
(a)PatientswerestratifiedintoaPIK3CAmutationpositiveorPIK3CAmutationnegativegroup.
TimefrominitiationoftreatmenttofirstprogressionwasanalyzedadjustingforreceptorstatusofthemetastaticbiopsyAND.
(b)PatientswerestratifiedintotwogroupsbasedonthepresenceorabsenceofmutationalheterogeneitydefinedashavingdifferentmutationsidentifiedinthecfDNAandmetDNAassays.
TimefrominitiationoftreatmenttofirstprogressionwasanalyzedadjustingforreceptorstatusofthemetastaticbiopsyAND.
67SupplementaryTable1:AssaysperformedpersampleinMETAMORPHstudyStudyIDSubgroupTumorAssaycfDNAAssay30113-13-0001tumor/cfDNACPD_FullGH_v130113-13-0003tumor/cfDNACPD_FullGH_v130113-13-0005tumor/cfDNACPD_FullGH_v130113-14-0001tumor/cfDNACPD_FullGH_v130113-14-0002tumor/cfDNACPD_FullGH_v130113-14-0004tumor/cfDNACPD_FullGH_v130113-14-0005tumor/cfDNACPD_FullGH_v130113-14-0006tumor/cfDNACPD_FullGH_v130113-14-0007tumor/cfDNACPD_FullGH_v130113-14-0008tumor/cfDNACPD_FullGH_v130113-14-0009tumor/cfDNACPD_FullGH_v130113-14-0010tumor/cfDNACPD_FullGH_v130113-14-0011tumor/cfDNACPD_FullGH_v130113-14-0013tumor/cfDNACPD_FullGH_v130113-14-0014tumor/cfDNACPD_FullGH_v130113-14-0015tumor/cfDNACPD_FullGH_v130113-14-0016tumor/cfDNACPD_FullGH_v130113-14-0019tumor/cfDNACPD_FullGH_v130113-14-0021tumor/cfDNACPD_FullGH_v130113-14-0024tumor/cfDNACPD_FullGH_v130113-14-0026tumor/cfDNACPD_FullGH_v130113-14-0027tumor/cfDNACPD_FullGH_v130113-14-0028tumor/cfDNACPD_FullGH_v130113-14-0029tumor/cfDNACPD_FullGH_v130113-14-0030tumor/cfDNACPD_FullGH_v130113-14-0018tumor/cfDNACPD_PPPGH_v130113-14-0020tumor/cfDNACPD_PPPGH_v130113-14-0022tumor/cfDNACPD_PPPGH_v130113-15-0010tumor/cfDNACPD_FullGH_v230113-15-0017tumor/cfDNACPD_FullGH_v230113-15-0012tumor/cfDNACPD_PPPGH_v230113-15-0014tumor/cfDNACPD_PPPGH_v230113-14-0017tumorCPD_Full30113-14-0031tumorCPD_Full30113-14-0032tumorCPD_Full30113-14-0033tumorCPD_Full30113-14-0034tumorCPD_Full30113-14-0036tumorCPD_Full30113-14-0039tumorCPD_Full30113-14-0040tumorCPD_Full30113-14-0042tumorCPD_Full30113-14-0043tumorCPD_Full30113-15-0002tumorCPD_Full30113-15-0003tumorCPD_Full30113-15-0004tumorCPD_Full30113-15-0006tumorCPD_Full30113-15-0007tumorCPD_Full30113-15-0009tumorCPD_Full30113-15-0013tumorCPD_Full30113-14-0023tumorCPD_PPP30113-14-0037tumorCPD_PPP30113-14-0044tumorCPD_PPP30113-15-0011tumorCPD_PPP30113-13-0006cfDNAGH_v230113-15-0015cfDNAGH_v230113-15-0018cfDNAGH_v28SupplementaryTable2:GenesfoundontheclinicalassaysusedinstudyGeneCPD_Full1CPD_PPPGH_v1GH_v22AKT1XXXXALKXXXXBRAFXXXXEGFRXXXXERBB2XXXXHRASXXXXIDH1XXXXKITXXXXKRASXXXXMETXXXXNOTCH1XXXXNRASXXXXPDGFRAXXXXPIK3CAXXXXPTENXXXXRETXXXXTP53XXXXAPCXXXATMXXXCDH1XXXCTNNB1XXXFBXW7XXXFGFR1XXXFGFR2XXXFGFR3XXXGNA11XXXGNAQXXXGNASXXXHNF1AXXXJAK2XXXJAK3XXXMLH1XXXMPLXXXNPM1XXXPTPN11XXXSMAD4XXXSMOXXXSRCXXXSTK11XXXVHLXXXARXXCDKN2AXXCSF1RXXXERBB4XXEZH2XXFLT3XXIDH2XXXKDRXXMAP2K1XXMYCXXPROCXRB1XXSMARCB1XXTERTXX1GenesarenotcoveredinfullontheCPDpanelsandmayincludeonlycertainexons.
2OthergenesontheGH_v2assayincludeARAF,ARID1A,BRCA1,BRCA2,CCND1,CCND2,CCNE1,CDK4,CDK6,CDKN2B,ESR1,GATA3,MAP2K2,NF1,NFE2L2,NTRK1,RAF1,RHEB,RHOA,RIT1,ROS19SupplementaryTable3:ReceptordiscordancebetweenprimaryandmetastaticbreasttumorbiopsiesCategoryn%Averagetimetorecurrence(months)HR+>HR-512.
5%89HR->HR+25.
0%228Her2+>Her2-12.
5%124Her2->Her2+00.
0%n/aConcordant3382.
5%7310SupplementaryTable4:ReanalysisoftumorsequencingdataforsinglenucleotidevariantsidentifiedcoveredandreportableonbothassaysbutdetectedonlybycfDNAassayPatientIDGeneVariantcfDNAAFTumorAADTumorTDTumorAAF30113-14-0001EGFRp.
K860R0.
40%851510.
16%30113-14-0001JAK3p.
G721R0.
50%05242nd30113-14-0002JAK2p.
V617F0.
20%1328690.
45%30113-14-0002RETp.
A641V0.
30%895240.
08%30113-14-0005NPM1p.
K292T0.
40%759870.
12%30113-14-0009TP53p.
R175G0.
60%740890.
17%30113-14-0018TP53p.
V272M0.
30%100742710.
13%30113-14-0020TP53p.
R175H0.
80%10309070.
03%30113-14-0024JAK2p.
V617F0.
30%120150.
05%30113-14-0026JAK2p.
V617F0.
30%1870250.
26%cfDNA:cellfreeDNA,AF:allelefrequency,AAD:alternatealleledepth,TD:totaldepth,AAF:alternateallelefrequency11SupplementaryTable5isincludedasanExcelspreadsheet12SupplementaryTable6:Multivariateanalysisofpanel-specificmutationalloadandTP53mutationstatuswithrespecttotimetoprogressiononstandardtherapyVariablecoefexp(coef)se(coef)zzPanel-specificmutationalloadmultivariateanalysisHighmutationalload1.
18283.
26350.
47722.
480.
013ReceptorHR+/Her2--0.
50950.
60080.
4822-1.
060.
291ReceptorHer2+-0.
61400.
54120.
6292-0.
980.
329Priortreatmentlines-0.
02510.
97520.
1027-0.
240.
807Chemotherapyorhormonaltherapy1.
05222.
86410.
57041.
840.
065PresenceofTP53mutationmultivariateanalysisPresenceofTP53mutation1.
04302.
83780.
40232.
590.
0095ReceptorHR+/Her2--0.
18840.
82820.
5051-0.
370.
7092ReceptorHer2+-0.
62500.
53530.
6226-1.
000.
3154Priortreatmentlines-0.
00050.
99950.
0963-0.
010.
9959Chemotherapyorhormonaltherapy0.
77032.
16050.
52601.
460.
1431 
		  
		  
		      
			  
		  
			  			   
			      
			        
			          
			          ftlcloud(超云)目前正在搞暑假促销,美国圣何塞数据中心的云服务器低至9元/月,系统盘与数据盘分离,支持Windows和Linux,免费防御CC攻击,自带10Gbps的DDoS防御。FTL-超云服务器的主要特色:稳定、安全、弹性、高性能的云端计算服务,快速部署,并且可根据业务需要扩展计算能力,按需付费,节约成本,提高资源的有效利用率。活动地址:https://www.ftlcloud.com...
			         
			       
				  
			     
							   
			      
			        
			          
			          云基yunbase怎么样?云基成立于2020年,目前主要提供高防海内外独立服务器,欢迎各类追求稳定和高防优质线路的用户。业务可选:洛杉矶CN2-GIA+高防(默认500G高防)、洛杉矶CN2-GIA(默认带50Gbps防御)、香港CN2-GIA高防(双向CN2GIA专线,突发带宽支持,15G-20G DDoS防御,无视CC)。目前,美国洛杉矶CN2-GIA高防独立服务器,8核16G,最高500G ...
			         
			       
				  
			     
							   
			      
			        
			          
			          VPSMS最近在做两周年活动,加上双十一也不久了,商家针对美国洛杉矶CN2 GIA线路VPS主机提供月付6.8折,季付6.2折优惠码,同时活动期间充值800元送150元。这是一家由港人和国人合资开办的VPS主机商,提供基于KVM架构的VPS主机,美国洛杉矶安畅的机器,线路方面电信联通CN2 GIA,移动直连,国内访问速度不错。下面分享几款VPS主机配置信息。CPU:1core内存:512MB硬盘:...
			         
			       
				  
			     
							
			   
			   
zpanel为你推荐
	主机租赁电脑出租怎么收费的?美国vps服务器请问国外VPS服务器去哪里买呀,急求?拜托了各位 谢谢网站空间域名关于网站的域名和空间?台湾主机香港,美国,台湾,韩国,日本主机到底哪个好成都虚拟空间虚拟主机哪家最好~~~虚拟空间哪个好虚拟内存设在哪个盘最好美国网站空间论坛选择空间可以选美国网站空间吗?香港虚拟主机推荐一下香港的虚拟主机公司!上海虚拟主机谁能告诉我杭州哪个公司的虚拟主机最好,机房最好是上海或浙江的.淘宝虚拟主机请问在淘宝的代购国外虚拟主机可以买吗?
vps侦探 域名备案中心 budgetvm 阿里云搜索 512au 标准机柜尺寸 发包服务器 789电视 nerds 多线空间 重庆电信服务器托管 申请免费空间 rewritecond 重庆联通服务器托管 香港ip shuangcheng 碳云 标准机柜 comodo 德国代理ip 更多