1DataFileUploadingThreemethodsareofferedfordatauploading:AsperaCommandLine,FTPandAsperaConnectplugin.
Pleasechooseonetouploadyourdata.
Ifyouneedanyhelpduringdatafileuploading,pleasecontacttheGSAWorkingTeamatgsa@big.
ac.
cnorQQgroup:548170081.
NOTICE:1.
Uniquefilenamesshouldbeusedforallfiles,andeachfilemustbelistedintheGSAmetadatafileyouuploaded.
2.
Filesmustbecompressedusinggziporbzip2.
3.
Uploadedfileswillberemovedaftertheyarearchived.
AsperaCommandLineUseAsperaCommandLinetouploadfiles.
YoumayusethefollowingcommandtouploadfilesviaAsperaComandLine:[path/to/ascp/]-P33001-i[path/to/key/file]-QT-l100m-k1-d[path/to/folder/containing/files]aspsub@submit.
big.
ac.
cn:uploads/zhangss@big.
ac.
cn_a657feb7Where:[path/to/ascp/]:MicrosoftWindows:C:\ProgramFiles\Aspera\AsperaConnect\bin\ascp.
exeorC:\users\[username]\AppData\Local\Programs\Aspera\AsperaConnect\bin\ascp.
exeMacOSX:/Applications/Aspera/Connect.
app/Contents/Resources/ascp(foradmin'sinstallation)or/Users/[username]/Applications/Aspera/Connect.
app/Contents/Resources/ascp(fornon-admin'sinstallation)2Linux:/opt/aspera/bin/ascpor/home/[username]/aspera/connect/bin/ascp[path/to/key/file]mustbeanabsolutepath,e.
g.
:/home/keys/aspera.
openssh[path/to/folder/containing/files]needstospecifythelocalfolderthatcontainsallthefilestoupload.
GetthekeyfileNotice:1)Pleasemakeanewsubdirectoryforeachnewsubmission.
Yoursubmissionsubfolderisatemporaryholdingareaandwillberemovedoncethewholesubmissioniscomplete.
2)Donotuploadcomplexdirectorystructuresorfilesthatdonotcontainsequencedata.
FTPUploadWerecommendtouseanFTPclientsoftwaretouploadfiles.
FTPUsernameandPasswordaresametotheBIGSubaccount.
NOTICE:Pleaseusethebinarymodetotransferfiles.
IfyouareusingtheFTPclientsoftware(suchasFileZillaClient)tologintotheFTPserver,followthetool'sinstructiontosetthetransfermode;IfyouareusingFTPcommand,typethebinarycommandbeforethemputcommand.
TransmittingyourdatafilestotheGSAFTPsiteAddress:ftp://submit.
big.
ac.
cnUser:SameasyoulogintheBIGSubPassword:SameasyoulogintheBIGSubAfteryouconnectedtotheFTPserver:1.
NavigatetotheGSAfolder.
32.
Uploadyourfileshere.
WebbrowseruploadviaAsperaConnectpluginUsetheAsperaConnectplugintouploadfiles.
IfyoudonothaveAsperaConnectplugininstalledinyourcomputer,pleaseDownloadAsperaConnectandinstallitfirst.
1.
OnopeningtheFileUploadpage,yourbrowsermayaskyouforpermissiontoexecuteanexternalapplicationcalled'IBMAsperaLauncher',click'LaunchApplication'toallowit.
2.
AfterselectingtouploadfilesviaAspera,clickthe'ClicktoUploadFiles'buttontoselectfilesonyourcomputer.
Youcanselectmultiplefilesatonetime.
3.
Afteryourfileshavebeenselected,apromptwillappearaskingyoutoconfirmtheAsperaconnectiontoBIGD,click'allow'tostartthefiletransfer.
4.
Youcanselectthe'Autofinishsubmission'checkboxtoallowbackendupload.
Thiswillallowyoutoturntothenextpagebeforetheuploadingiscompleted.
DONOTclosetheAsperaConnectTransferwindowwhenyouarestilluploadingyourfiles.
4Notice:OnlyoneAsperaconnectionshouldbecreatedatanygivenmoment.
'Autofinishsubmission'toallowbackendupload.
Clickthe'ClicktoUploadFiles'buttontoselectfilesonyourcomputer.
5ReleaseoflinkedBioProject/BioSample/GSAReleaserulesoflinkedBioProject,BioSample,andGSAareasfollows:1.
ThereleaseoftheBioProjectrecordsDONOTtriggerthereleaseoftheotherlinkeddata.
2.
ThereleaseoftheBioSamplerecordsJUSTtriggersthereleaseofitsBioProject.
3.
ThereleaseoftheGSAnucleotidesequencedataDOtriggerthereleaseofthelinkedBioProjectandBioSamplerecords.
Notice:Therefore,pleasecarefullyfillinthe'releasetime'ofaBioProject,BioSampleandGSA.
Oncepublished,therepresentativedataorinformationcanberetrievedordownloadedbyotherusers.
6StatusandOperationGSAStatusandOperationNo.
StatusDescriptionOperation1UnfinishedattheGeneralInfostepFinishedtheSubmitterstepandenterthegeneralinfostep.
Edit[1];Delete2UnfinishedattheSampleTypestepFinishedtheGeneralinfostep.
IfnotcreatedGSArelatedBiologicalSample(s),entertheSampletypestep.
Edit[1];Delete3UnfinishedattheAttributesstepFinishedSampletypestep,entertheAttributesstepEdit[1];Delete4UnfinishedattheMetadatastepFinishedtheAttributesstep,entertheGSAmetadatastep.
Edit[1];Delete5UnfinishedattheFileUploadstepFinishedtheGSAmetadatastep,entertheFileUploadstep.
Edit[1];Delete6UnfinishedattheOverviewstepEntertheoverviewstep.
Edit[1];Delete7UncheckedAlltheinformationaresubmitted,waitingforcheck.
Edit[1];Delete8CheckedfailedDatafile(s)processederror.
Edit[1];Delete;ReloaddatafileviaFTPorAsperaCommand7Line[2]9CheckedOKDatafile(s)ProcessedsucceedandGSAAccessionnumberisassigned.
ReleaseNow;Share10DeletedDeleted[1]:YoucanclicktheGSASubmissionIDtoentertheOverviewpagetoeditGSArelatedmetadata.
Formoredetail,pleasesee'HowtoEdit,DeleteorAddNewData'.
[2]:Formoredetailsfordatafileupload,pleasesee'DataFileUpload'.
ExperimentStatusandOperationNo.
StatusDescriptionOperation1UncheckedMetadatasubmittedandwaitingforcheck.
Edit[1];Delete2CheckedOKMetadataCheckedOKEdit[1];Delete3CheckedfailedMetadataCheckedfailedEdit[1];Delete5DeletedDeleted[1]:YoucanclicktheGSASubmissionIDtoentertheOverviewpagetoedittheExperimentmetadata.
Formoredetails,pleasesee'HowtoEdit,DeleteorAddNewData'.
RunStatusandOperationNo.
StatusDescriptionOperation1UncheckedMetadatasubmittedandwaitingforcheck.
Edit[1];Delete82CheckedOKMetadataCheckedOKEdit[1];Delete3CheckedfailedMetadataCheckedfailedEdit[1];Delete4UploadedSucceedDatafile(s)uploadedsucceed,waitingforprocessing.
5ProcessingDatafile(s)underprocessing.
6ProcessedsucceedDatafile(s)processedsucceed7ProcessederrorDatafile(s)processederrorEdit[1];Delete;ReloaddatafileviaFTPorAsperaCommandLine[2]8DeletedDeleted[1]:YoucanclicktheGSASubmissionIDtoentertheOverviewpagetoeditRunmetadata.
Formoredetail,pleasesee'HowtoEdit,DeleteorAddNewData'.
[2]:Formoredetailfordatafileupload,pleasesee'DataFileUpload'.
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